FastQCFastQC Report
Mon 6 Feb 2017
SRR2077330_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077330_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1521536
Sequences flagged as poor quality0
Sequence length46
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31830.20919649617228905No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8650.025.951281
GTATCAA22850.024.5600221
TATCAAC30900.017.6820222
ATCAACG31600.017.0291523
TCAACGC31900.016.8046344
CAACGCA32600.016.3786745
AACGCAG33250.016.0537386
TAGACTG2650.015.8474155
ACCTCGC1000.001021289613.9944118
TACCGTC1000.001021289613.9944117
GTATATG3600.013.9185631
ACGCAGA37800.013.80400857
GTATTAG2801.8189894E-1213.6004251
GTATAAT3550.013.5500151
CGCAGAG38600.013.5179178
GTGTAGC3450.013.361821
CCCTATA1551.2136676E-512.9120762
GAGTACT18450.012.89457812
GTAAGAC2051.01670594E-712.6857833
AGAGTAC38200.012.61276211
Produced by FastQC (version 0.11.5)