FastQCFastQC Report
Mon 6 Feb 2017
SRR2077330_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077330_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1521536
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14600.042.2706451
GTATCAA30600.037.3907621
TATCAAC41250.027.3900762
TCAACGC41550.027.1044244
ATCAACG41850.027.075733
CAACGCA42100.026.7494455
CGAATCG658.6844206E-426.5248237
AACGCAG43450.025.8360256
TATAACG700.001300310324.7514023
CGCTACG1752.419256E-1023.6488728
TATCTCG1752.4374458E-1023.64498536
ACGCAGA50050.022.196077
CGCAGAG50800.021.7918578
CGCTATC1756.2409526E-921.6752833
GTACATG40250.020.845931
ACGCTAT1851.1836164E-820.50364532
AGAGTAC51100.020.3154811
CAGAGTA53950.019.81697810
TACATGG40650.019.7800332
GAGTACT33700.019.34253512
Produced by FastQC (version 0.11.5)