FastQCFastQC Report
Mon 6 Feb 2017
SRR2077329_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077329_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1984741
Sequences flagged as poor quality0
Sequence length46
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42700.21514142147514462No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT19960.1005672780478662No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12350.025.8005371
GTATCAA29500.023.4366571
TATCAAC37400.018.1421852
TATAACG902.2638205E-517.7913532
ATCAACG38550.017.5397683
TCAACGC38700.017.521714
CAACGCA39300.017.3024855
AACGCAG41250.016.5267836
CGTGCCG750.002449213815.99366110
ACGCAGA47400.014.1294217
CGCAGAG48000.013.9111538
CCCTACG1050.001463334113.3435142
ATAGGAC3750.013.3364523
AGAGTAC46150.013.08290211
GAGTACT22600.013.00402412
GTACCGA1551.2277087E-512.8981146
GTACATG49300.012.7638391
CAGAGTA49150.012.56877210
GCAGAGT50900.012.4508619
GTATAGT2903.8198777E-1112.4386451
Produced by FastQC (version 0.11.5)