FastQCFastQC Report
Mon 6 Feb 2017
SRR2077329_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077329_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1984741
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA36550.037.7510571
GGTATCA17000.037.7273451
ATCAACG50500.026.3436763
TATCAAC51100.026.2410722
TCAACGC50700.026.2397564
CAACGCA51250.025.9568425
AACGCAG53150.025.0922166
ACGCAGA61450.021.5626077
CGCAGAG63500.020.7494438
AGAGTAC63800.019.95157611
TCGTATG4250.019.47236440
CAGAGTA67850.018.96306610
CGTATGC4250.018.66101641
GTACATG49400.018.5272271
GAGTACT41350.018.5203412
TAGATCG950.007651813318.2331185
CGCGATC950.007836818.15773623
GCAGAGT72250.018.0455749
AGTACTT44400.017.71427513
TACATGG50450.017.3073622
Produced by FastQC (version 0.11.5)