FastQCFastQC Report
Mon 6 Feb 2017
SRR2077328_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077328_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2287514
Sequences flagged as poor quality0
Sequence length46
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69220.30259924092267854No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32720.14303737594611443No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT29970.13101559159856507No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16150.026.8025991
GTATCAA42750.024.469781
TATCAAC57500.017.967892
TCAACGC58250.017.6542744
ATCAACG58500.017.6491583
CAACGCA59600.017.3162215
AACGCAG61700.016.7858146
CGTGCCG1402.4896508E-715.70950410
GTCTACG1301.93921E-615.4153121
CCTAACG800.003727621915.016772
CGTCGTA800.003763046814.99543510
GTCCTAA3200.014.4036831
ACGCAGA72100.014.2259057
CGCAGAG74000.013.6985328
GAGTACT35700.013.66530712
GTATTAC3100.013.5754191
GTATAGA3600.013.3599381
GATCGTA1050.001475277913.32927512
GTATAAT5700.013.008361
AGTACTT37550.012.9920513
Produced by FastQC (version 0.11.5)