##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2077328_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2287514
Sequences flagged as poor quality	0
Sequence length	46
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.166856683718656	31.0	31.0	34.0	26.0	34.0
2	30.205416447724474	31.0	31.0	34.0	26.0	34.0
3	30.27820245034566	33.0	31.0	34.0	26.0	34.0
4	33.66080863330235	37.0	35.0	37.0	28.0	37.0
5	33.68930463376399	37.0	35.0	37.0	28.0	37.0
6	33.75250905568228	37.0	35.0	37.0	29.0	37.0
7	33.622196410601205	37.0	35.0	37.0	28.0	37.0
8	33.57770662824359	37.0	35.0	37.0	28.0	37.0
9	35.05176405477737	39.0	37.0	39.0	27.0	39.0
10	34.984871786577045	39.0	35.0	39.0	27.0	39.0
11	35.032653789222714	39.0	37.0	39.0	27.0	39.0
12	34.95479022204891	39.0	35.0	39.0	27.0	39.0
13	34.92848655789648	39.0	35.0	39.0	27.0	39.0
14	35.98059946299782	40.0	37.0	41.0	27.0	41.0
15	36.114440829651755	40.0	37.0	41.0	27.0	41.0
16	36.10311587164057	40.0	37.0	41.0	27.0	41.0
17	36.12884336445591	40.0	37.0	41.0	27.0	41.0
18	36.096290995377515	40.0	37.0	41.0	27.0	41.0
19	36.19604776189348	40.0	37.0	41.0	27.0	41.0
20	36.185920610759105	40.0	37.0	41.0	27.0	41.0
21	36.2008722132411	40.0	37.0	41.0	27.0	41.0
22	36.14738008160824	40.0	37.0	41.0	27.0	41.0
23	36.10014627232883	40.0	37.0	41.0	27.0	41.0
24	36.13165034181212	40.0	37.0	41.0	27.0	41.0
25	36.0763016095202	40.0	37.0	41.0	27.0	41.0
26	36.10444045369777	40.0	37.0	41.0	27.0	41.0
27	36.08346265858919	40.0	37.0	41.0	27.0	41.0
28	36.02034260773923	40.0	37.0	41.0	27.0	41.0
29	35.97983487751332	40.0	37.0	41.0	27.0	41.0
30	35.890177284160885	40.0	36.0	41.0	27.0	41.0
31	35.856936831862015	40.0	36.0	41.0	27.0	41.0
32	35.821743167473514	40.0	36.0	41.0	27.0	41.0
33	35.771150690225284	40.0	36.0	41.0	26.0	41.0
34	35.71183389478709	40.0	36.0	41.0	26.0	41.0
35	35.68320237602917	40.0	36.0	41.0	26.0	41.0
36	35.621775866726935	40.0	36.0	41.0	25.0	41.0
37	35.53071150602794	40.0	36.0	41.0	25.0	41.0
38	35.49233971901374	40.0	35.0	41.0	25.0	41.0
39	35.40362813080051	40.0	35.0	41.0	25.0	41.0
40	35.37752249822296	40.0	35.0	41.0	25.0	41.0
41	35.238100400697	40.0	35.0	41.0	24.0	41.0
42	35.18348084427024	40.0	35.0	41.0	24.0	41.0
43	35.077394499006346	40.0	35.0	41.0	23.0	41.0
44	34.99039568719579	40.0	35.0	41.0	23.0	41.0
45	34.88486409263506	40.0	35.0	41.0	22.0	41.0
46	34.770930800860675	40.0	35.0	41.0	20.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.29846941231588886
1101	2	0.31196744613337657
1101	3	0.3481502042446891
1101	4	0.34665656694773617
1101	5	0.23241872510247674
1101	6	0.2032985277117234
1101	7	0.2434509214627809
1101	8	0.3668944685744506
1101	9	0.37506788585024964
1101	10	0.42442588954764204
1101	11	0.4601389915496128
1101	12	0.4140441107748103
1101	13	0.5123806750197772
1101	14	0.7034288577179311
1101	15	0.5076856426054093
1101	16	0.5069020319606139
1101	17	0.43611801613559464
1101	18	0.48543431018170224
1101	19	0.40158708624758077
1101	20	0.33048697039609465
1101	21	0.3906003686787898
1101	22	0.541893537116998
1101	23	0.46469757783153653
1101	24	0.34491459628048915
1101	25	0.4301210607379957
1101	26	0.46712076580008244
1101	27	0.5196843031639915
1101	28	0.5048557222722252
1101	29	0.42907077530053783
1101	30	0.47836376795945057
1101	31	0.40138726037418593
1101	32	0.44180492089581236
1101	33	0.42800333919907274
1101	34	0.30589389936936584
1101	35	0.37542603240311934
1101	36	0.37216283439143893
1101	37	0.34522587583153097
1101	38	0.37922108130252496
1101	39	0.47964417425051664
1101	40	0.4440663941651124
1101	41	0.4535596285292769
1101	42	0.3665137681205408
1101	43	0.4152221450903042
1101	44	0.4110995953468617
1101	45	0.35708567024401816
1101	46	0.41894837314814026
1102	1	0.6259373903562384
1102	2	0.6694175211336209
1102	3	0.6471372271934754
1102	4	0.7069787458735419
1102	5	0.5421475721148568
1102	6	0.5842400896723845
1102	7	0.6607018497755845
1102	8	0.6564105786576846
1102	9	0.8470828267785535
1102	10	0.800864215393581
1102	11	0.7689649174558895
1102	12	0.7872694397109612
1102	13	0.8652475490140574
1102	14	1.1648704314269906
1102	15	1.0396776627914264
1102	16	1.1311735366421587
1102	17	1.0012486620927419
1102	18	0.9598968968450734
1102	19	1.0004288080622317
1102	20	0.9277830201304624
1102	21	0.8619454615252309
1102	22	0.959796122446356
1102	23	1.0097108004621305
1102	24	1.0316796109205058
1102	25	1.0540122584604745
1102	26	0.9108048279256025
1102	27	0.9769563331125468
1102	28	1.0212051317068713
1102	29	1.1026796704282944
1102	30	1.0854888671576077
1102	31	0.9909399799763463
1102	32	1.0874369050332007
1102	33	1.1485402560712075
1102	34	1.0897109002842242
1102	35	1.038271880557751
1102	36	1.0529494312145218
1102	37	0.9784987365054079
1102	38	1.0117212006813858
1102	39	1.1248234661481433
1102	40	0.9953672827228601
1102	41	1.1209356204160628
1102	42	1.0661165586299575
1102	43	1.0295166084121874
1102	44	1.091922384810296
1102	45	1.1596057391578398
1102	46	1.0938132097431037
1103	1	0.5330431113052541
1103	2	0.5872703217680169
1103	3	0.6526861541680518
1103	4	0.6585237986030705
1103	5	0.5811193609392404
1103	6	0.581537957374529
1103	7	0.6300723800555588
1103	8	0.6792994223535516
1103	9	0.8861156993381272
1103	10	0.7921544794508861
1103	11	0.7801876821569564
1103	12	0.7498700998542205
1103	13	0.7147194856098693
1103	14	1.008361831422313
1103	15	0.9983568934436136
1103	16	0.9123452616761014
1103	17	0.8571087869230212
1103	18	0.8496241122460759
1103	19	0.7850009219837659
1103	20	0.8621223940477805
1103	21	0.7758387924981207
1103	22	0.692647321004344
1103	23	0.6898549653926054
1103	24	0.8650400722599869
1103	25	0.8086957715579999
1103	26	0.7510170920765447
1103	27	0.8304184641228929
1103	28	0.8175750021361949
1103	29	0.8381613101937333
1103	30	0.8042128974258773
1103	31	0.9301739167828487
1103	32	0.8391112211879843
1103	33	0.8854218838393635
1103	34	0.8576181276239865
1103	35	0.8896092485876252
1103	36	0.9094123464376125
1103	37	0.8454874400378785
1103	38	0.8766948724942054
1103	39	0.9551544302616293
1103	40	0.833061763094129
1103	41	1.0020414335393397
1103	42	0.8933856547955088
1103	43	0.8974219337434306
1103	44	0.7395628700858197
1103	45	0.796451045878662
1103	46	0.758712044836038
1104	1	0.5649375833844772
1104	2	0.4565058424566111
1104	3	0.5062858194661111
1104	4	0.5120643821034463
1104	5	0.5121443874192764
1104	6	0.5293143640907729
1104	7	0.492201535000774
1104	8	0.4794913670131251
1104	9	0.6200429589677512
1104	10	0.7920882793643287
1104	11	0.8403074153350119
1104	12	0.7771004021252423
1104	13	0.7066034795678675
1104	14	0.9925188338254429
1104	15	0.8491879892614733
1104	16	0.8609935698012023
1104	17	0.8311239182696895
1104	18	0.6505571597563815
1104	19	0.7091805569863823
1104	20	0.8146282808726824
1104	21	0.8006820655727651
1104	22	0.736475337450436
1104	23	0.7887926853477296
1104	24	0.76647908774369
1104	25	0.613366098686086
1104	26	0.6670946017004695
1104	27	0.7942095971551666
1104	28	0.8080053525268482
1104	29	0.8816194425540402
1104	30	0.9900331592080676
1104	31	0.895935392176348
1104	32	0.8864911748052151
1104	33	0.8233722935680703
1104	34	0.8472737412489622
1104	35	0.8310494884170154
1104	36	0.8713292340085061
1104	37	0.878380252132132
1104	38	0.8540477694954163
1104	39	0.9079859788026639
1104	40	0.9476431685575548
1104	41	0.960100653431823
1104	42	0.8766964706083584
1104	43	0.8349186415348271
1104	44	0.7273738681268682
1104	45	0.7519201873845489
1104	46	0.7257934289446055
1105	1	0.4754554219539777
1105	2	0.36961428294044296
1105	3	0.5234407964419567
1105	4	0.4952846071119055
1105	5	0.6910080378735799
1105	6	0.6843721553416415
1105	7	0.5134899770540926
1105	8	0.6124131950193927
1105	9	0.7797069680620865
1105	10	0.6727864481410535
1105	11	0.5726466267171517
1105	12	0.6599739966894518
1105	13	0.7081848427728374
1105	14	1.0131447109695557
1105	15	0.874206686494631
1105	16	0.9236813496364533
1105	17	0.6748735795517007
1105	18	0.6257099784753777
1105	19	0.7156433613068884
1105	20	0.7652584806003233
1105	21	0.7093788695056702
1105	22	0.7666343972516714
1105	23	0.8314081562080133
1105	24	0.795561967321369
1105	25	0.8394385177850552
1105	26	0.7838593321936784
1105	27	0.8029099996280138
1105	28	0.9200905937005643
1105	29	0.7798887182633294
1105	30	0.9228719173042421
1105	31	0.8239065830459396
1105	32	0.8501978617161967
1105	33	0.8525291383162141
1105	34	0.7641638570135569
1105	35	0.8539335144114801
1105	36	0.8871529246277206
1105	37	0.8898886430168176
1105	38	0.8378703814197621
1105	39	0.9553752814273011
1105	40	0.8900426836651363
1105	41	0.866695216341455
1105	42	0.870101430783798
1105	43	0.9840600862662399
1105	44	0.9173133909778102
1105	45	0.8966505158358657
1105	46	0.9628088779535062
1106	1	0.18819811486650906
1106	2	0.31349975552869935
1106	3	0.31298236143300784
1106	4	0.3957338235318417
1106	5	0.19049418287656295
1106	6	0.3431229823032993
1106	7	0.3325804819074136
1106	8	0.416428161669792
1106	9	0.28564525774268645
1106	10	0.37063667318308546
1106	11	0.3495793310173525
1106	12	0.3888116952958214
1106	13	0.31381750911414485
1106	14	0.6337127929607718
1106	15	0.5869589233581394
1106	16	0.5178812303370677
1106	17	0.5025250084329969
1106	18	0.4033190018472155
1106	19	0.35625937996097434
1106	20	0.3785050507429375
1106	21	0.3399694014724801
1106	22	0.3885768081838279
1106	23	0.5371976470165336
1106	24	0.5051096057357682
1106	25	0.483135497341479
1106	26	0.402100425409877
1106	27	0.42794508449325974
1106	28	0.44111613461483046
1106	29	0.5138597610484297
1106	30	0.5668808105312877
1106	31	0.49227033772302065
1106	32	0.48486843272644364
1106	33	0.5209384436684203
1106	34	0.5510370200741264
1106	35	0.32026353989816414
1106	36	0.29712201524102966
1106	37	0.40752955186123785
1106	38	0.3997514073944899
1106	39	0.5342653633100625
1106	40	0.40711616124227845
1106	41	0.5166851301205355
1106	42	0.3896402844251412
1106	43	0.5765121224898664
1106	44	0.5032234826214363
1106	45	0.56891427593564
1106	46	0.455003444408689
1107	1	0.3056150472983674
1107	2	0.24196410047065697
1107	3	0.34938445353659375
1107	4	0.3052436249520909
1107	5	0.26184733166255114
1107	6	0.26372348406798096
1107	7	0.21237271731158813
1107	8	0.32172730696893126
1107	9	0.31369534916591135
1107	10	0.4242225047092134
1107	11	0.4491161767034555
1107	12	0.3854183202905048
1107	13	0.3529630633940286
1107	14	0.5187575690801793
1107	15	0.479278606991258
1107	16	0.4729975291847097
1107	17	0.4078003411710114
1107	18	0.5216650493872876
1107	19	0.5047689687690706
1107	20	0.38365834410518573
1107	21	0.38131651207332595
1107	22	0.3763800994199542
1107	23	0.362595529399492
1107	24	0.4552067269672193
1107	25	0.41223606288980363
1107	26	0.310610949711446
1107	27	0.4014530149265667
1107	28	0.4464767673485994
1107	29	0.46345282138776156
1107	30	0.5825836637863802
1107	31	0.5273278705715967
1107	32	0.4543747473073836
1107	33	0.457474481428342
1107	34	0.4585662117227258
1107	35	0.5268841122500802
1107	36	0.49210734500248066
1107	37	0.4618990717829945
1107	38	0.3456915358122288
1107	39	0.46163943313189293
1107	40	0.43312185924797575
1107	41	0.5211555982924949
1107	42	0.5466398166784003
1107	43	0.5026933530890076
1107	44	0.46737214103875147
1107	45	0.44638198014961006
1107	46	0.45369715534120303
1108	1	0.2059215618445691
1108	2	0.19965789095620678
1108	3	0.20885166474142736
1108	4	0.18306675737400013
1108	5	0.08341011101020257
1108	6	0.14438722468931076
1108	7	0.11798551339362007
1108	8	0.1832089987089276
1108	9	0.15346153385416983
1108	10	0.24855704828426894
1108	11	0.2283663917084695
1108	12	0.1312389616197649
1108	13	0.14504897219800483
1108	14	0.24651778870303787
1108	15	0.2947418104177828
1108	16	0.23831478339054968
1108	17	0.25733919247227277
1108	18	0.365374404965344
1108	19	0.2240698529374967
1108	20	0.20033955626747968
1108	21	0.2418068506162001
1108	22	0.3335517348561723
1108	23	0.2431575927698404
1108	24	0.31144250803914986
1108	25	0.3643068895682191
1108	26	0.20014603048131363
1108	27	0.21868286058897013
1108	28	0.33946463809986227
1108	29	0.37914755238788445
1108	30	0.08999339345199076
1108	31	0.1999313589486178
1108	32	0.28495926440399444
1108	33	0.29999442499746465
1108	34	0.2776802639424645
1108	35	0.14978013455129258
1108	36	0.24041907898089931
1108	37	0.24733888944179228
1108	38	0.3131071542852908
1108	39	0.2700117074401689
1108	40	0.257703181984283
1108	41	0.324136860022449
1108	42	0.2955065389851228
1108	43	0.38413979671533127
1108	44	0.3952850883656964
1108	45	0.4161499441897618
1108	46	0.4402079733770563
1109	1	-0.1410037478755406
1109	2	-0.16415567867896286
1109	3	-0.030057061075467573
1109	4	-0.08084611569049116
1109	5	-0.024614024182355365
1109	6	-0.0855264052528355
1109	7	-0.13593000898112706
1109	8	-0.16197689138102334
1109	9	-0.11996055865898114
1109	10	-0.08897596774123429
1109	11	-0.1381001445937926
1109	12	-0.1878910853341509
1109	13	-0.09728700564984649
1109	14	0.052201731633417126
1109	15	-0.1891092803818637
1109	16	-0.033321150985727854
1109	17	-0.14505415291822032
1109	18	-0.08940589106586572
1109	19	-0.03162864808387411
1109	20	-0.25934470604268967
1109	21	-0.11284120246666163
1109	22	-0.14499175337689252
1109	23	-0.22606990953469364
1109	24	-0.08710977466395065
1109	25	-0.02476240448081768
1109	26	-0.08270420447904314
1109	27	-0.15962831024003066
1109	28	-0.09724311738429492
1109	29	-0.16365478514155285
1109	30	-0.10447814069100758
1109	31	-0.14527233628675162
1109	32	-0.11063982826487262
1109	33	-0.11998205790288097
1109	34	-0.14698652349184016
1109	35	-0.1872787399902336
1109	36	-0.2255376817003807
1109	37	-0.14493310513908142
1109	38	-0.09603020788082262
1109	39	-0.0833927675174877
1109	40	-0.09491381272496824
1109	41	-0.12666581421500922
1109	42	-0.0054040749720627446
1109	43	-0.03491216990669699
1109	44	-0.11378198321965272
1109	45	-0.11882742936528956
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2216	4	-1.568753238873029
2216	5	-1.513891408193956
2216	6	-1.699162152146215
2216	7	-1.757367767712072
2216	8	-1.7456925892180841
2216	9	-2.160700759507435
2216	10	-2.130852926005147
2216	11	-2.06340697035251
2216	12	-2.3943826223094575
2216	13	-2.376254535533242
2216	14	-4.044357222259926
2216	15	-2.9984718224561675
2216	16	-2.7729051725041245
2216	17	-2.4674496237334154
2216	18	-2.8042548185485288
2216	19	-2.5610414825453702
2216	20	-2.453701950779127
2216	21	-2.511030091170845
2216	22	-2.5122552567578254
2216	23	-2.671977391035213
2216	24	-2.6103688316056832
2216	25	-2.576324675159789
2216	26	-2.464720477684999
2216	27	-2.7513709987132344
2216	28	-2.6600467268768
2216	29	-2.7377688538678555
2216	30	-2.6417990474088455
2216	31	-2.791431336553998
2216	32	-2.7302812374917167
2216	33	-2.6061625203092547
2216	34	-2.6134825131577344
2216	35	-2.4677892251956948
2216	36	-2.7531773251952956
2216	37	-2.633868723126078
2216	38	-2.7260669589170234
2216	39	-2.6764256564768445
2216	40	-2.719475416636975
2216	41	-2.8391159860641437
2216	42	-2.7119304918305716
2216	43	-2.692106852649651
2216	44	-2.799926187596732
2216	45	-2.821342446540257
2216	46	-2.9317849878591957
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	86087.0
3	2611.0
4	8304.0
5	6240.0
6	5005.0
7	4106.0
8	3464.0
9	2977.0
10	2971.0
11	2818.0
12	2788.0
13	2841.0
14	2937.0
15	3091.0
16	3337.0
17	3706.0
18	4197.0
19	5211.0
20	7053.0
21	10294.0
22	13079.0
23	14962.0
24	15439.0
25	16015.0
26	17385.0
27	20177.0
28	22518.0
29	24621.0
30	27397.0
31	33083.0
32	40881.0
33	50756.0
34	66285.0
35	89805.0
36	122888.0
37	177312.0
38	316969.0
39	1047904.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.106283853713855	18.344896939297968	13.83033844625606	23.71848076073212
2	15.107544565299163	20.576930142395515	39.30808972369427	25.007435568611054
3	17.958809263847478	26.244112109631995	29.678291969372474	26.118786657148057
4	12.463324124634116	17.02705398024008	38.61516968442563	31.89445221070017
5	13.561950443843266	38.46637260793014	34.33892324822074	13.632753700005859
6	30.92348287267313	37.75828257225967	18.009332401900053	13.308902153167148
7	27.38798538500748	32.97147033854219	22.161525568805263	17.479018707645068
8	26.597695139789305	34.34851109107966	20.32490292955584	18.72889083957519
9	26.93924496199805	15.890613128487956	19.293521263695	37.876620645818996
10	14.552566672815992	29.13936264433791	33.85159609952114	22.456474583324955
11	33.835639913023485	23.84378849703215	24.158016082087368	18.162555507857
12	23.406982427211375	25.195124488855587	30.41913623260885	20.97875685132419
13	27.379242269118354	22.857084153364745	26.385368570421864	23.378305007095037
14	22.46722861586858	21.559824333315554	27.37167947387426	28.60126757694161
15	23.297364123117166	29.29973700684412	24.229512238624324	23.17338663141439
16	23.12326831660921	28.697441851722	25.472193831382018	22.707096000286775
17	22.072593312119594	28.27557987699294	26.80280007396686	22.84902673692061
18	22.111320315085376	28.138711706674556	27.752964253224143	21.997003725015922
19	23.107123856453587	27.622686841724477	27.80292641215714	21.46726288966479
20	23.504136003294462	26.97652519766345	27.619505183446897	21.899833615595192
21	23.58068122055404	26.826430969292048	27.59282922971532	22.00005858043859
22	23.40059995261231	26.54082117093054	27.470826408056958	22.587752468400193
23	23.07704749835083	26.773292019142204	27.43125606827683	22.71840441423014
24	22.8731677675726	26.852254197795855	27.44726799008529	22.827310044546252
25	23.046496927221174	26.714596595593633	27.452387010132433	22.786519467052763
26	22.85223420276677	26.895853779554578	27.52627863108496	22.725633386593692
27	22.879874069458563	27.08114430634353	27.233572732539706	22.805408891658193
28	22.87039995943182	26.91803784644156	27.36800368261168	22.84355851151494
29	22.974688524590164	27.059016393442626	27.326120218579238	22.640174863387976
30	23.152521359600193	27.028768574895867	27.269075006032793	22.549635059471143
31	23.14729568689579	26.674080893764092	27.374585463393565	22.80403795594655
32	23.056168183238942	26.965099529837094	27.171131866378435	22.80760042054553
33	23.0992990117968	26.83711587179807	27.16227647394409	22.901308642461043
34	23.238173761593668	26.776995929102025	27.375040219352854	22.609790089951456
35	23.191772587678557	26.970699133323862	27.103509327970798	22.734018951026787
36	23.14361977526832	26.950572324139767	27.20057057498286	22.70523732560906
37	23.300731903627078	26.664183414410648	27.28324770922165	22.751836972740623
38	23.20867459386481	26.71339274432709	27.245258568111396	22.8326740936967
39	23.173656230249936	27.021827032630114	27.156100131867554	22.648416605252397
40	23.35610395084205	26.69881136327267	27.261168664342385	22.683916021542892
41	23.13590102928286	26.93040837143062	27.12535625993985	22.80833433934667
42	23.153908870300356	26.95784687145159	27.046989024755096	22.841255233492955
43	23.03942938823734	26.701819760737994	27.179880268427496	23.078870582597162
44	23.127280391518955	26.903897348711435	27.011467111638172	22.957355148131434
45	23.229594919948614	26.9185528395133	26.91269339448145	22.939158846056635
46	23.314179922543904	26.684530162795976	26.90464644750944	23.096643467150685
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1071.0
1	1162.5
2	1254.0
3	1442.0
4	1630.0
5	1750.0
6	1870.0
7	1870.0
8	1749.5
9	1629.0
10	1879.0
11	2129.0
12	2795.5
13	3462.0
14	6095.5
15	8729.0
16	11680.5
17	14632.0
18	14948.5
19	15265.0
20	15265.0
21	16818.5
22	18372.0
23	22510.5
24	26649.0
25	33153.5
26	39658.0
27	45158.5
28	50659.0
29	57348.0
30	64037.0
31	64037.0
32	71449.0
33	78861.0
34	87975.0
35	97089.0
36	105181.0
37	113273.0
38	120414.5
39	127556.0
40	134987.0
41	142418.0
42	148734.0
43	155050.0
44	155050.0
45	159702.0
46	164354.0
47	167173.5
48	169993.0
49	171175.5
50	172358.0
51	168721.0
52	165084.0
53	158334.5
54	151585.0
55	142142.5
56	132700.0
57	132700.0
58	122355.5
59	112011.0
60	99473.0
61	86935.0
62	74867.5
63	62800.0
64	52436.5
65	42073.0
66	34170.0
67	26267.0
68	21043.0
69	15819.0
70	15819.0
71	12494.5
72	9170.0
73	7203.5
74	5237.0
75	4031.5
76	2826.0
77	2175.5
78	1525.0
79	1136.5
80	748.0
81	748.0
82	545.5
83	343.0
84	260.5
85	178.0
86	143.5
87	109.0
88	72.5
89	36.0
90	27.5
91	19.0
92	14.0
93	9.0
94	9.0
95	10.0
96	11.0
97	9.5
98	8.0
99	15.5
100	23.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1899441926912797
2	0.0527209888114346
3	0.06408703946729943
4	0.05551878589595518
5	0.03916915918328806
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	8.743115889126798E-5
16	0.0
17	1.31146738336902E-4
18	3.0600905611943797E-4
19	1.31146738336902E-4
20	0.0017486231778253598
21	0.0026666503461836738
22	0.0
23	1.31146738336902E-4
24	1.7486231778253596E-4
25	3.497246355650719E-4
26	3.9344021501070596E-4
27	0.023562697321196722
28	3.0600905611943797E-4
29	6.120181122388759E-4
30	7.868804300214119E-4
31	8.743115889126799E-4
32	3.9344021501070596E-4
33	0.0012677518039233859
34	0.0025355036078467718
35	0.0017049075983797256
36	0.03051347445305253
37	0.008349675674116094
38	0.01237150898311442
39	0.049354889194120774
40	0.0155627462826457
41	0.0551690612603901
42	0.05586851053152025
43	0.024699302386783206
44	0.02736595273296688
45	0.02657907230294547
46	0.023650128480087993
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
46	2287514.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.06813223935524
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.54079350079607	50.31961944192894
2	12.407634905021578	15.898679877451244
3	4.047034922292248	7.778579057361254
4	1.7460323033287877	4.474601140154191
5	0.9137664502792926	2.9271654886189964
6	0.5539632363884525	2.1294833930805943
7	0.3808578925580959	1.7080597677369964
8	0.25990371458706485	1.332123644453097
9	0.19647242229413175	1.132885901963419
>10	0.9188218561244434	9.837072993352788
>50	0.027169024811277434	1.1411671675110202
>100	0.007207502553742046	0.6862573722199062
>500	1.36907585917294E-4	0.05578618870951683
>1k	1.3690758591620018E-4	0.2749399504856223
>5k	6.845379295810009E-5	0.3035786149723206
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6922	0.30259924092267854	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3272	0.14303737594611443	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2997	0.13101559159856507	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	8.743115889126799E-5	0.0
5	0.0	0.0	0.0	8.743115889126799E-5	0.0
6	0.0	0.0	0.0	8.743115889126799E-5	0.0
7	0.0	0.0	0.0	8.743115889126799E-5	0.0
8	0.0	0.0	0.0	8.743115889126799E-5	0.0
9	0.0	0.0	0.0	8.743115889126799E-5	4.3715579445633996E-5
10	0.0	0.0	0.0	1.31146738336902E-4	4.3715579445633996E-5
11	0.0	0.0	0.0	1.31146738336902E-4	4.3715579445633996E-5
12	0.0	0.0	0.0	1.31146738336902E-4	4.3715579445633996E-5
13	0.0	0.0	0.0	1.31146738336902E-4	4.3715579445633996E-5
14	0.0	0.0	0.0	1.31146738336902E-4	4.3715579445633996E-5
15	0.0	0.0	0.0	1.7486231778253598E-4	1.7486231778253598E-4
16	0.0	0.0	0.0	1.7486231778253598E-4	1.7486231778253598E-4
17	0.0	0.0	0.0	1.7486231778253598E-4	1.7486231778253598E-4
18	0.0	0.0	0.0	1.7486231778253598E-4	2.1857789722816998E-4
19	0.0	0.0	0.0	1.7486231778253598E-4	2.1857789722816998E-4
20	0.0	0.0	0.0	1.7486231778253598E-4	2.1857789722816998E-4
21	0.0	0.0	0.0	1.7486231778253598E-4	2.1857789722816998E-4
22	0.0	0.0	0.0	1.7486231778253598E-4	2.1857789722816998E-4
23	0.0	0.0	0.0	1.7486231778253598E-4	2.62293476673804E-4
24	0.0	0.0	0.0	2.62293476673804E-4	2.62293476673804E-4
25	0.0	0.0	0.0	2.62293476673804E-4	3.0600905611943797E-4
26	0.0	0.0	0.0	3.0600905611943797E-4	3.0600905611943797E-4
27	0.0	0.0	0.0	3.0600905611943797E-4	3.0600905611943797E-4
28	0.0	0.0	0.0	5.24586953347608E-4	3.0600905611943797E-4
29	0.0	0.0	0.0	0.00109288948614085	3.0600905611943797E-4
30	0.0	0.0	0.0	0.0025355036078467718	3.4972463556507197E-4
31	0.0	0.0	0.0	0.004284126785672132	3.4972463556507197E-4
32	0.0	0.0	0.0	0.006557336916845099	3.4972463556507197E-4
33	0.0	0.0	0.0	0.008874262627463702	3.4972463556507197E-4
34	0.0	0.0	0.0	0.012415224562560055	3.4972463556507197E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1615	0.0	26.802599	1
GTATCAA	4275	0.0	24.46978	1
TATCAAC	5750	0.0	17.96789	2
TCAACGC	5825	0.0	17.654274	4
ATCAACG	5850	0.0	17.649158	3
CAACGCA	5960	0.0	17.316221	5
AACGCAG	6170	0.0	16.785814	6
CGTGCCG	140	2.4896508E-7	15.709504	10
GTCTACG	130	1.93921E-6	15.415312	1
CCTAACG	80	0.0037276219	15.01677	2
CGTCGTA	80	0.0037630468	14.995435	10
GTCCTAA	320	0.0	14.403683	1
ACGCAGA	7210	0.0	14.225905	7
CGCAGAG	7400	0.0	13.698532	8
GAGTACT	3570	0.0	13.665307	12
GTATTAC	310	0.0	13.575419	1
GTATAGA	360	0.0	13.359938	1
GATCGTA	105	0.0014752779	13.329275	12
GTATAAT	570	0.0	13.00836	1
AGTACTT	3755	0.0	12.99205	13
>>END_MODULE