FastQCFastQC Report
Mon 6 Feb 2017
SRR2077328_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077328_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2287514
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24350.10644743595011877No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22350.042.3545951
GTATCAA53300.040.009521
TATCAAC75100.028.2003462
ATCAACG75350.027.9675483
TCAACGC75450.027.8833184
CAACGCA77750.027.1012655
AACGCAG80450.026.3197926
ACGCAGA93000.022.4448537
CGCAGAG96350.021.4764948
GTACATG69600.020.6754191
GAGTACT64850.020.58137112
AGAGTAC96800.020.4507811
TACATGG72150.020.0333182
CAGAGTA101800.019.8524310
AGTACTT67300.019.7808813
GCAGAGT103550.019.6834499
GTACTTT69150.019.19927814
ACATGGG74100.018.522993
TATGCCG2551.376975E-917.57925843
TACTTTT76650.017.3656715
Produced by FastQC (version 0.11.5)