FastQCFastQC Report
Mon 6 Feb 2017
SRR2077327_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077327_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1919662
Sequences flagged as poor quality0
Sequence length46
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69630.36272010385161557No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT32390.16872761975806158No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30990.16143466922822872No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTCG457.813996E-526.6558848
GGTATCA17300.026.0784761
GTATCAA44600.023.9628331
CCCGCTA500.004495064719.991919
TATCAAC58850.018.030912
ATCAACG59800.017.6325283
TCAACGC60450.017.4388394
CAACGCA61650.017.0629485
AACGCAG63550.016.6101196
GCGTATC852.9076837E-416.512961
TAATTCG1453.6983874E-715.17062625
ACCGTTA1852.6451744E-814.0600935
ACGCAGA74000.013.9943387
CGCAGAG75350.013.7436118
ATAAGGC3500.013.7198183
CGTATTA2056.9758244E-913.65336828
GTGGTAT6350.013.5781491
GAGTACT32350.013.4103412
TTACACC4200.013.3355834
GACCGCG1050.0014762413.3279417
Produced by FastQC (version 0.11.5)