##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2077327_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1919662
Sequences flagged as poor quality	0
Sequence length	46
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.25367434475444	31.0	31.0	34.0	26.0	34.0
2	30.28104530901794	31.0	31.0	34.0	26.0	34.0
3	30.43646277313402	33.0	31.0	34.0	26.0	34.0
4	33.81287434975532	37.0	35.0	37.0	28.0	37.0
5	33.892170600866194	37.0	35.0	37.0	29.0	37.0
6	33.9272194792625	37.0	35.0	37.0	30.0	37.0
7	33.84268063857075	37.0	35.0	37.0	29.0	37.0
8	33.72094722925181	37.0	35.0	37.0	28.0	37.0
9	35.179438359461194	39.0	37.0	39.0	28.0	39.0
10	35.12548615329157	39.0	37.0	39.0	27.0	39.0
11	35.12743545478319	39.0	37.0	39.0	27.0	39.0
12	35.059276060056405	39.0	37.0	39.0	27.0	39.0
13	35.01112383325815	39.0	37.0	39.0	27.0	39.0
14	36.056006213593854	40.0	37.0	41.0	27.0	41.0
15	36.19703572816464	40.0	37.0	41.0	27.0	41.0
16	36.18182002873422	40.0	37.0	41.0	27.0	41.0
17	36.2181514245737	40.0	37.0	41.0	27.0	41.0
18	36.19370701717281	40.0	37.0	41.0	27.0	41.0
19	36.30086911133314	40.0	37.0	41.0	27.0	41.0
20	36.30712854658789	40.0	37.0	41.0	27.0	41.0
21	36.32556304182715	40.0	37.0	41.0	27.0	41.0
22	36.28385622052215	40.0	37.0	41.0	27.0	41.0
23	36.24620584248685	40.0	37.0	41.0	27.0	41.0
24	36.281950676733715	40.0	37.0	41.0	27.0	41.0
25	36.2324252915357	40.0	37.0	41.0	27.0	41.0
26	36.269974089188615	40.0	37.0	41.0	27.0	41.0
27	36.257284355266705	40.0	37.0	41.0	27.0	41.0
28	36.201800108560775	40.0	37.0	41.0	27.0	41.0
29	36.169604336596755	40.0	37.0	41.0	27.0	41.0
30	36.07745738572728	40.0	36.0	41.0	27.0	41.0
31	36.047774556145825	40.0	36.0	41.0	27.0	41.0
32	36.0100715646817	40.0	36.0	41.0	27.0	41.0
33	35.96461772957948	40.0	36.0	41.0	27.0	41.0
34	35.91428438964776	40.0	36.0	41.0	27.0	41.0
35	35.87791965460586	40.0	36.0	41.0	27.0	41.0
36	35.81315304465057	40.0	36.0	41.0	27.0	41.0
37	35.72167600337976	40.0	36.0	41.0	27.0	41.0
38	35.678828877166914	40.0	35.0	41.0	27.0	41.0
39	35.594550498994096	40.0	35.0	41.0	26.0	41.0
40	35.56857717660713	40.0	35.0	41.0	26.0	41.0
41	35.42860878633843	40.0	35.0	41.0	25.0	41.0
42	35.36649941500118	40.0	35.0	41.0	25.0	41.0
43	35.25783861950698	40.0	35.0	41.0	25.0	41.0
44	35.16806083570962	40.0	35.0	41.0	24.0	41.0
45	35.05326666881982	40.0	35.0	41.0	24.0	41.0
46	34.93265845758264	40.0	35.0	41.0	23.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.36218212835562724
1101	2	0.27672258181479137
1101	3	0.41133457722879996
1101	4	0.4788614711215544
1101	5	0.41755380867169833
1101	6	0.4047855581756963
1101	7	0.512190038813543
1101	8	0.4777826384775139
1101	9	0.6139490164322439
1101	10	0.5124270658361922
1101	11	0.5966714909247344
1101	12	0.5716287262750512
1101	13	0.5655274688633938
1101	14	0.7888243930437255
1101	15	0.6283963422263099
1101	16	0.5409287142944805
1101	17	0.6229417378485209
1101	18	0.6514334948763221
1101	19	0.5564839104700638
1101	20	0.4192722701549343
1101	21	0.48576587095654133
1101	22	0.5879443434704896
1101	23	0.5738343937458268
1101	24	0.5473901241435186
1101	25	0.6101176151566818
1101	26	0.6798009167690466
1101	27	0.630621782129964
1101	28	0.5777000259133871
1101	29	0.5812595213951681
1101	30	0.6806059150779475
1101	31	0.638692975389958
1101	32	0.6190665455363273
1101	33	0.5910472946830865
1101	34	0.5513085921593657
1101	35	0.5498153016333092
1101	36	0.5551237775912696
1101	37	0.6177475706409652
1101	38	0.5541831224344378
1101	39	0.6154476356976062
1101	40	0.5504117137944249
1101	41	0.5198481575960372
1101	42	0.46262267424694414
1101	43	0.42629531268114107
1101	44	0.4351830186099761
1101	45	0.2975447366538191
1101	46	0.3121064385693657
1102	1	0.47086755471326924
1102	2	0.4705932838538516
1102	3	0.6018296511108865
1102	4	0.5103408526453777
1102	5	0.5384998871443045
1102	6	0.5084726995853046
1102	7	0.517616919853026
1102	8	0.48704271739527627
1102	9	0.6945752847795958
1102	10	0.6935369534679126
1102	11	0.7218298278063315
1102	12	0.6609693812579991
1102	13	0.6685428760250076
1102	14	1.004294602344153
1102	15	0.8388846894195865
1102	16	0.8606589951691177
1102	17	0.8009248767981703
1102	18	0.7836411025504049
1102	19	0.7630370512449147
1102	20	0.7835619909653033
1102	21	0.7358394065631941
1102	22	0.7036208263228403
1102	23	0.8005089861022086
1102	24	0.8621719226689066
1102	25	0.8426758571596551
1102	26	0.751473265338106
1102	27	0.7265720297343279
1102	28	0.7315686931224121
1102	29	0.8088939820697405
1102	30	0.8154808324463545
1102	31	0.8026476702462304
1102	32	0.8528824032979685
1102	33	0.9171390898184271
1102	34	0.8878465157701925
1102	35	0.7756436503673925
1102	36	0.7368215495191279
1102	37	0.9518702684891309
1102	38	0.9078506031666436
1102	39	0.8994753280788359
1102	40	0.887670373137837
1102	41	0.9595404816803708
1102	42	0.8770776315348741
1102	43	0.7837385781080286
1102	44	0.8452194305850824
1102	45	0.716413165531371
1102	46	0.6328597422641451
1103	1	0.807696032164845
1103	2	0.7346333634356235
1103	3	0.8887664413436802
1103	4	0.8481408574741138
1103	5	0.8515935590785801
1103	6	0.8938970663395196
1103	7	0.9287268523174319
1103	8	0.9257923147861078
1103	9	1.1197496973811667
1103	10	1.1306056094390868
1103	11	0.9924784767604251
1103	12	1.0052663550240624
1103	13	1.017466195096425
1103	14	1.2756985127001101
1103	15	1.2040351483811875
1103	16	1.147661666901513
1103	17	1.127483160478512
1103	18	1.188371959287295
1103	19	1.0737850592606293
1103	20	1.0944759227577876
1103	21	1.1293714357596158
1103	22	1.1085176068365072
1103	23	0.9844480782063414
1103	24	1.1600592968968897
1103	25	1.1033911684311235
1103	26	1.0016253193254911
1103	27	1.0219580901850378
1103	28	1.1066336114421915
1103	29	1.136159111313063
1103	30	1.1089743087652337
1103	31	1.0719460695184253
1103	32	1.0492368832656283
1103	33	1.1025379772558352
1103	34	1.209629482333213
1103	35	1.1374374918726886
1103	36	1.1969367869211212
1103	37	1.167212104859523
1103	38	1.1389789567661381
1103	39	1.1469236721030356
1103	40	1.1083231830287446
1103	41	1.107596630697394
1103	42	1.2064338883766652
1103	43	1.1766266894896305
1103	44	1.1252766768565508
1103	45	1.2021370426820184
1103	46	1.0505350936449815
1104	1	0.5535474397749667
1104	2	0.5329899385906494
1104	3	0.5548765323868352
1104	4	0.5665666310498167
1104	5	0.5887833683487429
1104	6	0.5051162354966863
1104	7	0.608513996650295
1104	8	0.6025130645745378
1104	9	0.6188108054192512
1104	10	0.6826048143549599
1104	11	0.7092985217147998
1104	12	0.6890607936514996
1104	13	0.6485486639932816
1104	14	0.9857249552708467
1104	15	0.8453267183812159
1104	16	0.8036582777550123
1104	17	0.6053598441344121
1104	18	0.541235466833065
1104	19	0.6339017519114236
1104	20	0.8218132643056535
1104	21	0.7813361569129711
1104	22	0.8460542615200239
1104	23	0.7160710436894533
1104	24	0.6486023554208984
1104	25	0.6731808827329004
1104	26	0.6535207717855798
1104	27	0.7187015079023595
1104	28	0.8287007599111007
1104	29	0.8682072259497389
1104	30	0.9506376207524312
1104	31	0.7822046626009893
1104	32	0.7556317389106724
1104	33	0.8625583462672353
1104	34	0.9341905547330498
1104	35	0.6552347775438037
1104	36	0.7013822809926253
1104	37	0.6092347105469855
1104	38	0.6762121342616396
1104	39	0.7208444106478495
1104	40	0.7073094005665723
1104	41	0.7328966695480901
1104	42	0.7769326028902626
1104	43	0.7963247482596003
1104	44	0.7802186969525025
1104	45	0.7214700353606176
1104	46	0.7274659082749295
1105	1	0.1257747792297863
1105	2	0.09961870575336818
1105	3	0.3754110493890046
1105	4	0.288461857697456
1105	5	0.3733346140695417
1105	6	0.4256756659222276
1105	7	0.3940650051476595
1105	8	0.42372947487778134
1105	9	0.5908971011505173
1105	10	0.5760905000547538
1105	11	0.5041004997016572
1105	12	0.5517272843030057
1105	13	0.5625181961668346
1105	14	0.8433869247811785
1105	15	0.5587565920572573
1105	16	0.6484871046702452
1105	17	0.45334999588793323
1105	18	0.43592985059309086
1105	19	0.5385543089120404
1105	20	0.5558463405973058
1105	21	0.405671261316094
1105	22	0.4397711656251815
1105	23	0.5724771682228251
1105	24	0.4721376496027716
1105	25	0.5238216837739031
1105	26	0.5652061599138918
1105	27	0.5254343853829084
1105	28	0.7832878571399533
1105	29	0.6084086078010529
1105	30	0.7003393544686318
1105	31	0.6376219425911458
1105	32	0.6074023289617827
1105	33	0.7444473792988617
1105	34	0.6556661738216931
1105	35	0.7956343253304325
1105	36	0.779691324565313
1105	37	0.7414577422624689
1105	38	0.7097618802142023
1105	39	0.7514690626291625
1105	40	0.5717836032625812
1105	41	0.6571037824793535
1105	42	0.6855504637304293
1105	43	0.6959628584523685
1105	44	0.5502362852338649
1105	45	0.44456726024347404
1105	46	0.570232926078603
1106	1	0.18086369820668224
1106	2	0.14727421921543993
1106	3	0.17832932180898808
1106	4	0.2546901345117405
1106	5	0.4091242731420408
1106	6	0.3627715028287142
1106	7	0.2655732812485496
1106	8	0.29178547423968837
1106	9	0.19133254330301952
1106	10	0.3372923788542508
1106	11	0.28456534949529555
1106	12	0.22373993910060364
1106	13	0.20901652687695815
1106	14	0.4910728812949259
1106	15	0.4869708110678914
1106	16	0.4759640519232633
1106	17	0.32644049324664337
1106	18	0.4114048637586549
1106	19	0.34494417912902975
1106	20	0.4053755508168564
1106	21	0.30662173351779387
1106	22	0.2687269169344262
1106	23	0.34065605446786407
1106	24	0.41375102235106453
1106	25	0.4302885657465154
1106	26	0.3382357463986594
1106	27	0.4275487036245167
1106	28	0.45140038298187335
1106	29	0.3679698067569248
1106	30	0.47851664053374066
1106	31	0.34801563144185366
1106	32	0.36685367036339045
1106	33	0.39884294476925675
1106	34	0.4068796848889491
1106	35	0.24194209591318128
1106	36	0.2926817636140626
1106	37	0.2833991766677215
1106	38	0.19823909845744225
1106	39	0.292483705514087
1106	40	0.30043408185510145
1106	41	0.44386395315860483
1106	42	0.2884534279275144
1106	43	0.25494385594409863
1106	44	0.25306441748700337
1106	45	0.255042295702566
1106	46	0.1505758302714213
1107	1	0.5077360117554832
1107	2	0.4136939443308769
1107	3	0.4167109213148201
1107	4	0.45116184844712137
1107	5	0.43639827638092044
1107	6	0.40831889587117587
1107	7	0.38181642106833635
1107	8	0.4371710657060248
1107	9	0.6064679372374968
1107	10	0.5246519780480199
1107	11	0.5619706250388887
1107	12	0.47830062751607727
1107	13	0.5431823795484689
1107	14	0.6739036750346088
1107	15	0.6997719447476243
1107	16	0.5971894833406921
1107	17	0.5124813134117829
1107	18	0.7132559991839358
1107	19	0.6717099786503908
1107	20	0.7525510766719776
1107	21	0.57921392871134
1107	22	0.6005836594861051
1107	23	0.6056889598214212
1107	24	0.6894324805744105
1107	25	0.5755694243564449
1107	26	0.4080501697516681
1107	27	0.5256794715015971
1107	28	0.6697958186242445
1107	29	0.6680444021995484
1107	30	0.7444241008617141
1107	31	0.6713327276201042
1107	32	0.5912142477762927
1107	33	0.6004259070846629
1107	34	0.574591542714451
1107	35	0.6662736758617527
1107	36	0.6257796261375788
1107	37	0.5350740117847224
1107	38	0.5408131470805628
1107	39	0.6516098202599707
1107	40	0.7029559350424321
1107	41	0.7412379370895934
1107	42	0.6517652945876264
1107	43	0.5038215400797768
1107	44	0.5755023276461628
1107	45	0.5423156149717769
1107	46	0.5455793605523951
1108	1	0.28859364949391164
1108	2	0.278504479068193
1108	3	0.18236096481896524
1108	4	0.26244275591933075
1108	5	0.16692377411951043
1108	6	0.18317552075770038
1108	7	0.1493588275074913
1108	8	0.3011155581891032
1108	9	0.26653961805984494
1108	10	0.4068567626957744
1108	11	0.2961771390773933
1108	12	0.1598018033401658
1108	13	0.10975988292239691
1108	14	0.2492271276743594
1108	15	0.3286815935089038
1108	16	0.27870951545025235
1108	17	0.2654410767923352
1108	18	0.34357852247818244
1108	19	0.33293391320292187
1108	20	0.19819514003987138
1108	21	0.2984653880531525
1108	22	0.39083105395151563
1108	23	0.34408560986761927
1108	24	0.383283849944803
1108	25	0.42434605549248516
1108	26	0.30625653293988364
1108	27	0.28244349047640327
1108	28	0.4449285534051981
1108	29	0.5375360098056348
1108	30	0.22973236072287762
1108	31	0.3337883816773015
1108	32	0.4857315133993936
1108	33	0.3161694238896544
1108	34	0.4867677196833853
1108	35	0.36974175082357164
1108	36	0.39945908005955744
1108	37	0.4557725563488546
1108	38	0.33131375452232703
1108	39	0.4630557202300736
1108	40	0.4949199249246874
1108	41	0.5755962442261549
1108	42	0.5163724508173431
1108	43	0.5225572800138849
1108	44	0.45336612600242887
1108	45	0.34720962917863574
1108	46	0.2959234877622876
1109	1	0.15297320744002363
1109	2	0.08129420085037609
1109	3	0.1628314211705728
1109	4	0.14214762644164125
1109	5	0.1437347161725242
1109	6	0.12172690746100301
1109	7	0.016538282299507046
1109	8	-0.0031877043222792167
1109	9	0.018104472688008855
1109	10	0.11203429124296349
1109	11	0.12613778964991695
1109	12	0.11403199836806976
1109	13	0.13515850858706102
1109	14	0.191732316079694
1109	15	0.10295526061941729
1109	16	-0.08008384479436614
1109	17	-0.06072409957835845
1109	18	0.1360335372934287
1109	19	0.08752321780748673
1109	20	-0.0953769388835326
1109	21	0.013487729335317056
1109	22	-0.02257696084092231
1109	23	-0.014378376546915206
1109	24	0.0799336224970375
1109	25	0.0434131600377512
1109	26	0.2322088224169434
1109	27	0.05762896609923018
1109	28	0.03726111659146625
1109	29	0.11736684059958691
1109	30	0.14886165595937229
1109	31	0.03026754647999752
1109	32	0.04913794930263293
1109	33	0.012110450623758595
1109	34	0.042760393637500727
1109	35	0.16535147397411976
1109	36	0.03704348484463793
1109	37	0.09948568270736047
1109	38	0.015855639821289458
1109	39	0.19198149664883601
1109	40	0.12272749692642293
1109	41	0.2303892255373725
1109	42	0.08008720869468533
1109	43	0.11746606407623261
1109	44	0.05635268263806381
1109	45	0.18982683100102804
1109	46	0.03932022226855736
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2216	14	-3.8917466117764405
2216	15	-2.763320950492094
2216	16	-2.3228408587353826
2216	17	-2.094383067490746
2216	18	-2.569855829444151
2216	19	-2.213552597330626
2216	20	-2.1241235257436415
2216	21	-2.1848902711957123
2216	22	-2.1616171350954403
2216	23	-2.3314521069244307
2216	24	-2.1985134439235665
2216	25	-2.2666150792901405
2216	26	-2.190058043329067
2216	27	-2.4932337838835608
2216	28	-2.4167418040401216
2216	29	-2.692103331917629
2216	30	-2.4274058358704025
2216	31	-2.4857865032330153
2216	32	-2.3424540899275783
2216	33	-2.2130334503042235
2216	34	-2.2264822580730836
2216	35	-2.246757100751111
2216	36	-2.4857692374396763
2216	37	-2.406691977575001
2216	38	-2.520344130068338
2216	39	-2.401636874156651
2216	40	-2.5023605973204894
2216	41	-2.683026831708318
2216	42	-2.504548826851142
2216	43	-2.4086352369323905
2216	44	-2.329691057999362
2216	45	-2.2817777299044693
2216	46	-2.3926402885633493
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	62648.0
3	2017.0
4	6619.0
5	5351.0
6	3991.0
7	3326.0
8	2753.0
9	2563.0
10	2338.0
11	2208.0
12	2163.0
13	2286.0
14	2317.0
15	2579.0
16	2681.0
17	2771.0
18	3256.0
19	4243.0
20	6335.0
21	9518.0
22	12344.0
23	14186.0
24	14341.0
25	13943.0
26	14938.0
27	17384.0
28	19572.0
29	20934.0
30	23357.0
31	27697.0
32	34304.0
33	43172.0
34	55881.0
35	75195.0
36	104076.0
37	151557.0
38	265444.0
39	879374.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.7498825656432	19.070139104910115	13.981577941965506	23.198400387481186
2	14.828533887470364	21.26083895053785	39.803998521884296	24.10662864010749
3	17.58772595130602	27.39877053089858	30.109847377148224	24.90365614064718
4	11.916380338423306	17.548568080794603	39.61358196632446	30.92146961445763
5	12.873803064114176	39.23614983495391	34.54481780308913	13.345229297842778
6	29.54629512903834	38.900806496143595	18.418450748100447	13.134447626717622
7	26.674591672909088	33.69900534573274	22.678940355125015	16.947462626233158
8	26.027238128378848	34.64854750471697	20.885499634831547	18.438714732072626
9	27.07877740977318	16.104762192510975	19.86021497534462	36.95624542237123
10	14.423111985339085	29.514518701729784	33.98546202404382	22.076907288887316
11	32.901729575310654	24.917198965234505	24.43346797509145	17.747603484363395
12	22.94414329189201	26.330364407900973	30.447026611976487	20.278465688230533
13	26.844152772727696	23.67338625237151	26.764347056929815	22.718113917970975
14	22.132229527906475	22.47869677057732	27.848287875678114	27.54078582583809
15	22.80777699812623	30.218929279700944	24.603458631497958	22.369835090674865
16	22.44473245810981	29.63344588786984	25.918833628003263	22.002988026017082
17	21.216717995307494	29.263003371437385	27.17367069933363	22.346607933921494
18	21.327016193098203	28.754587436739605	28.52032537266005	21.398070997502142
19	22.51672567906804	28.21280721046981	28.58771871791412	20.682748392548028
20	22.903899329656202	27.578958664563462	28.330673073516937	21.186468932263395
21	23.33824231913895	27.392088701397004	28.007578626689085	21.262090352774965
22	22.859024140708105	27.232814943464007	27.998314286577532	21.909846629250357
23	22.4961946346693	27.400141066794188	27.9884229378358	22.115241360700708
24	22.116068833237655	27.504071046136442	28.168357422743888	22.21150269788201
25	22.451147293670505	27.4581589958159	28.112706079447896	21.977987631065695
26	22.072142467934192	27.634261733673814	28.26151279975579	22.032082998636206
27	22.16751078596886	27.78830321599033	27.819879530627983	22.224306467412827
28	22.196633026801283	27.51709038172122	28.164136427166035	22.12214016431146
29	22.305304843298906	27.580142547412485	28.19452793802178	21.92002467126683
30	22.359395218676877	27.685144475306778	28.029584775447013	21.925875530569336
31	22.630703504996532	27.356768045525413	27.853009250215276	22.159519199262775
32	22.180223759889188	27.73598479303747	27.849547338651327	22.234244108422015
33	22.271855655441755	27.632631096946337	27.796464837736874	22.299048409875034
34	22.51493930249047	27.492275738551918	27.970812684511	22.021972274446615
35	22.50563004105515	27.655313857532743	27.90432238164672	21.93473371976539
36	22.560694093431646	27.589171725593392	27.598655584794564	22.2514785961804
37	22.70183761300463	27.209936332411395	27.99284814126171	22.095377913322263
38	22.431133057486978	27.306496838117226	27.95783739513792	22.30453270925787
39	22.519469093888407	27.64772901397939	27.696095548923687	22.136706343208516
40	22.613149631201157	27.34133990920833	28.072216528711184	21.97329393087933
41	22.360605220306613	27.51878021879502	27.872648954291563	22.2479656066068
42	22.628326951604848	27.481029430314756	27.664141427304067	22.226502190776326
43	22.301411176341553	27.259051917512643	27.815123029170287	22.62441387697552
44	22.302490409676924	27.5657826860408	27.668902758657342	22.46282414562493
45	22.534470028361795	27.486919861124644	27.517402407652213	22.461207702861348
46	22.56494479230224	27.30242230254475	27.522356890111382	22.61027601504163
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1054.0
1	1065.5
2	1077.0
3	1275.0
4	1473.0
5	1499.5
6	1526.0
7	1526.0
8	1469.0
9	1412.0
10	1580.0
11	1748.0
12	2413.5
13	3079.0
14	5534.5
15	7990.0
16	11314.0
17	14638.0
18	15163.0
19	15688.0
20	15688.0
21	17251.5
22	18815.0
23	23021.0
24	27227.0
25	33963.5
26	40700.0
27	46713.5
28	52727.0
29	58750.0
30	64773.0
31	64773.0
32	71671.5
33	78570.0
34	86809.0
35	95048.0
36	101264.5
37	107481.0
38	112362.0
39	117243.0
40	122118.0
41	126993.0
42	129359.5
43	131726.0
44	131726.0
45	132506.5
46	133287.0
47	134232.0
48	135177.0
49	135157.5
50	135138.0
51	130261.5
52	125385.0
53	118820.5
54	112256.0
55	105483.5
56	98711.0
57	98711.0
58	89958.5
59	81206.0
60	72449.0
61	63692.0
62	54587.5
63	45483.0
64	38272.0
65	31061.0
66	25099.0
67	19137.0
68	15760.5
69	12384.0
70	12384.0
71	9537.5
72	6691.0
73	5247.0
74	3803.0
75	3046.0
76	2289.0
77	1737.0
78	1185.0
79	946.0
80	707.0
81	707.0
82	603.0
83	499.0
84	378.5
85	258.0
86	227.5
87	197.0
88	126.0
89	55.0
90	36.5
91	18.0
92	14.5
93	11.0
94	11.0
95	10.0
96	9.0
97	9.5
98	10.0
99	17.5
100	25.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.19263807899515645
2	0.05224878129587396
3	0.06631375731769447
4	0.05823941923109381
5	0.0418823730427544
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	5.20925037845204E-5
16	0.0
17	3.1255502270712243E-4
18	8.855725643368468E-4
19	4.688325340606836E-4
20	0.00260462518922602
21	0.0027609027005795813
22	0.0
23	2.083700151380816E-4
24	4.167400302761632E-4
25	7.292950529832856E-4
26	8.334800605523264E-4
27	0.026150436899829238
28	7.81387556767806E-4
29	0.001041850075690408
30	0.0013544050983975305
31	0.0016148676173201326
32	7.81387556767806E-4
33	0.0017190526248891734
34	0.0031776427308557445
35	0.0025525326854414997
36	0.03188061231612649
37	0.008386893109307784
38	0.012293830893146814
39	0.05105065370882999
40	0.01682587872240009
41	0.060114749367336545
42	0.06063567440518175
43	0.02516067932792335
44	0.028129952043641014
45	0.027661119509580334
46	0.023702089221956782
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
46	1919662.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.04750879577955
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.43657488094934	44.543418188063384
2	13.803331976123486	16.301047325424335
3	4.784377728403144	8.475167580006492
4	2.1904477955731267	5.173619419032004
5	1.1373508263389513	3.3578866461068175
6	0.6765449563257291	2.396897655563027
7	0.4343909391064677	1.7954791958387264
8	0.2783829271136617	1.3150254669871038
9	0.20876450960195958	1.1094321795271482
>10	0.9671700967303606	9.93443514212261
>50	0.05773644973594916	2.3257893077681886
>100	0.02439489241736076	2.4702596771263114
>500	2.6601079020669674E-4	0.10513402381600136
>1k	1.7734052680446442E-4	0.33184235206464957
>5k	8.867026340223221E-5	0.3645658405531958
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6963	0.36272010385161557	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3239	0.16872761975806158	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3099	0.16143466922822872	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.20925037845204E-5
2	0.0	0.0	0.0	0.0	5.20925037845204E-5
3	0.0	0.0	0.0	0.0	5.20925037845204E-5
4	0.0	0.0	0.0	0.0	5.20925037845204E-5
5	0.0	0.0	0.0	0.0	5.20925037845204E-5
6	0.0	0.0	0.0	0.0	1.041850075690408E-4
7	0.0	0.0	0.0	0.0	1.041850075690408E-4
8	0.0	0.0	0.0	0.0	1.041850075690408E-4
9	0.0	0.0	0.0	0.0	1.041850075690408E-4
10	0.0	0.0	0.0	0.0	1.041850075690408E-4
11	0.0	0.0	0.0	0.0	1.041850075690408E-4
12	0.0	0.0	0.0	0.0	1.562775113535612E-4
13	0.0	0.0	0.0	0.0	1.562775113535612E-4
14	0.0	0.0	0.0	0.0	2.083700151380816E-4
15	0.0	0.0	0.0	0.0	2.60462518922602E-4
16	0.0	0.0	0.0	0.0	2.60462518922602E-4
17	0.0	0.0	0.0	0.0	2.60462518922602E-4
18	0.0	0.0	0.0	0.0	2.60462518922602E-4
19	0.0	0.0	0.0	0.0	2.60462518922602E-4
20	0.0	0.0	0.0	1.041850075690408E-4	2.60462518922602E-4
21	0.0	0.0	0.0	1.041850075690408E-4	2.60462518922602E-4
22	0.0	0.0	0.0	2.083700151380816E-4	2.60462518922602E-4
23	0.0	0.0	0.0	2.60462518922602E-4	2.60462518922602E-4
24	0.0	0.0	0.0	4.167400302761632E-4	3.125550227071224E-4
25	0.0	0.0	0.0	5.20925037845204E-4	3.125550227071224E-4
26	0.0	0.0	0.0	7.292950529832856E-4	3.125550227071224E-4
27	0.0	0.0	0.0	8.334800605523264E-4	3.125550227071224E-4
28	0.0	5.20925037845204E-5	0.0	0.0016148676173201324	3.125550227071224E-4
29	0.0	5.20925037845204E-5	0.0	0.00260462518922602	3.125550227071224E-4
30	0.0	5.20925037845204E-5	0.0	0.004011122791408071	3.125550227071224E-4
31	0.0	5.20925037845204E-5	0.0	0.006042730439004366	3.125550227071224E-4
32	0.0	5.20925037845204E-5	0.0	0.008595263124445866	3.646475264916428E-4
33	0.0	5.20925037845204E-5	0.0	0.012241738389362294	3.646475264916428E-4
34	0.0	5.20925037845204E-5	0.0	0.017242618752676252	4.167400302761632E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGTCG	45	7.813996E-5	26.655884	8
GGTATCA	1730	0.0	26.078476	1
GTATCAA	4460	0.0	23.962833	1
CCCGCTA	50	0.0044950647	19.99191	9
TATCAAC	5885	0.0	18.03091	2
ATCAACG	5980	0.0	17.632528	3
TCAACGC	6045	0.0	17.438839	4
CAACGCA	6165	0.0	17.062948	5
AACGCAG	6355	0.0	16.610119	6
GCGTATC	85	2.9076837E-4	16.51296	1
TAATTCG	145	3.6983874E-7	15.170626	25
ACCGTTA	185	2.6451744E-8	14.06009	35
ACGCAGA	7400	0.0	13.994338	7
CGCAGAG	7535	0.0	13.743611	8
ATAAGGC	350	0.0	13.719818	3
CGTATTA	205	6.9758244E-9	13.653368	28
GTGGTAT	635	0.0	13.578149	1
GAGTACT	3235	0.0	13.41034	12
TTACACC	420	0.0	13.335583	4
GACCGCG	105	0.00147624	13.327941	7
>>END_MODULE