FastQCFastQC Report
Mon 6 Feb 2017
SRR2077327_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077327_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1919662
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25260.13158566455969853No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26650.047.095321
GTATCAA63800.039.779271
TATCAAC91550.027.5607832
ATCAACG92300.027.3361173
TCAACGC92900.027.1968734
CAACGCA95450.026.4319085
AACGCAG97900.025.946076
ACGCAGA111150.022.5697147
CGCAGAG113950.022.0030828
AGAGTAC116150.020.87370711
GAGTACT76800.020.34231812
GTACATG81700.020.2424321
GCAGAGT122200.020.2071599
CAGAGTA120800.020.18440410
CGTATAC1055.842057E-419.8047393
AGTACTT81550.019.4534813
TACATGG83250.019.442462
GTACTTT82650.018.89903514
GTACCGT1501.631199E-518.4820046
CCGGCGT2801.2732926E-1118.4711969
Produced by FastQC (version 0.11.5)