FastQCFastQC Report
Mon 6 Feb 2017
SRR2077326_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077326_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2365944
Sequences flagged as poor quality0
Sequence length46
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69960.2956959251782798No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32000.13525256726279236No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT31940.13499896869917463No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15650.023.6914961
GTATCAA38950.023.4634841
TATCAAC49850.017.9881742
TCAACGC50550.017.49044
ATCAACG50750.017.4247883
CAACGCA51200.017.1880365
AACGCAG53400.016.5107366
GCGCAAT750.002448978615.99407411
ACGCCCG800.003756290314.99951721
GCGTGCG1557.888302E-714.1882919
TCGCGTC850.005627149714.1139117
ACGCAGA62750.013.9231297
GTATTAC3050.013.7992191
CGCAGAG63400.013.5911788
GGACCGT1955.3343683E-813.3283956
GAGTACT32250.013.20468812
GTGTAGC7050.012.792591
AGTACTT34150.012.587110513
AGAGTAC63250.012.58031911
TAGCCCT8500.012.472564
Produced by FastQC (version 0.11.5)