##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2077326_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2365944
Sequences flagged as poor quality	0
Sequence length	46
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.011769509337498	31.0	31.0	34.0	26.0	34.0
2	30.115243640593352	31.0	31.0	34.0	26.0	34.0
3	30.235725782182502	33.0	31.0	34.0	26.0	34.0
4	33.602694738337	37.0	35.0	37.0	28.0	37.0
5	33.6684866590249	37.0	35.0	37.0	28.0	37.0
6	33.71839316568778	37.0	35.0	37.0	28.0	37.0
7	33.62408915849234	37.0	35.0	37.0	28.0	37.0
8	33.539587581109274	37.0	35.0	37.0	28.0	37.0
9	35.009517131428304	39.0	37.0	39.0	27.0	39.0
10	34.946629336958104	39.0	35.0	39.0	27.0	39.0
11	34.950742705659984	39.0	37.0	39.0	27.0	39.0
12	34.87590915085057	39.0	35.0	39.0	27.0	39.0
13	34.830152784681296	39.0	35.0	39.0	27.0	39.0
14	35.873462770040206	40.0	36.0	41.0	26.0	41.0
15	36.00576598600812	40.0	37.0	41.0	27.0	41.0
16	35.979461897661146	40.0	37.0	41.0	27.0	41.0
17	35.99930302661432	40.0	37.0	41.0	27.0	41.0
18	35.96532208708236	40.0	37.0	41.0	27.0	41.0
19	36.06460719273153	40.0	37.0	41.0	27.0	41.0
20	36.0590766307233	40.0	37.0	41.0	27.0	41.0
21	36.07426803001255	40.0	37.0	41.0	27.0	41.0
22	36.0219523369953	40.0	37.0	41.0	27.0	41.0
23	35.97912757022144	40.0	37.0	41.0	27.0	41.0
24	36.00844356417565	40.0	37.0	41.0	27.0	41.0
25	35.94923886617773	40.0	37.0	41.0	27.0	41.0
26	35.98195012223451	40.0	37.0	41.0	27.0	41.0
27	35.956204796056035	40.0	37.0	41.0	27.0	41.0
28	35.8950761302888	40.0	36.0	41.0	27.0	41.0
29	35.86179427746388	40.0	36.0	41.0	27.0	41.0
30	35.76691291087194	40.0	36.0	41.0	26.0	41.0
31	35.73248648319656	40.0	36.0	41.0	26.0	41.0
32	35.70078285876589	40.0	36.0	41.0	26.0	41.0
33	35.64980785682163	40.0	36.0	41.0	25.0	41.0
34	35.5967829331548	40.0	36.0	41.0	25.0	41.0
35	35.56591491599125	40.0	36.0	41.0	25.0	41.0
36	35.501747716767596	40.0	36.0	41.0	25.0	41.0
37	35.418746174888334	40.0	35.0	41.0	25.0	41.0
38	35.377448916796	40.0	35.0	41.0	25.0	41.0
39	35.29922643984811	40.0	35.0	41.0	24.0	41.0
40	35.27057107015212	40.0	35.0	41.0	24.0	41.0
41	35.132621904829534	40.0	35.0	41.0	24.0	41.0
42	35.078028051382454	40.0	35.0	41.0	23.0	41.0
43	34.974981656370566	40.0	35.0	41.0	23.0	41.0
44	34.888562028518	40.0	35.0	41.0	22.0	41.0
45	34.77986080820172	40.0	35.0	41.0	20.0	41.0
46	34.66794269010594	40.0	35.0	41.0	18.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.5001011272824734
1101	2	0.48530012545806755
1101	3	0.6782002623595496
1101	4	0.612227822727732
1101	5	0.5447475828314694
1101	6	0.5198967753372017
1101	7	0.6817118944223424
1101	8	0.7347614915099356
1101	9	0.6438540253141056
1101	10	0.6697645057381649
1101	11	0.6743454283166059
1101	12	0.7050409401370743
1101	13	0.7839283901183336
1101	14	0.9733316863529069
1101	15	0.8287710151978231
1101	16	0.7508286217715252
1101	17	0.7992405188535372
1101	18	0.7966097162919752
1101	19	0.8007512134643733
1101	20	0.6738097707580479
1101	21	0.693329416915546
1101	22	0.8275972606776989
1101	23	0.8907191336775924
1101	24	0.8716172356895413
1101	25	0.8852492731611292
1101	26	0.9379016776072504
1101	27	0.9038713463038661
1101	28	0.7885447021502472
1101	29	0.8808450044544003
1101	30	0.8826679226241723
1101	31	0.8814609571951308
1101	32	0.9168472408386776
1101	33	0.8781041942381052
1101	34	0.8470416879630918
1101	35	0.9303422655640432
1101	36	0.9102611147710249
1101	37	0.8373788373702524
1101	38	0.7458250859619469
1101	39	0.8867264359502158
1101	40	0.8179146128392532
1101	41	0.7395082936581119
1101	42	0.7506852461179037
1101	43	0.7213129121636825
1101	44	0.7714478377286014
1101	45	0.848435138381987
1101	46	0.6913415120585995
1102	1	0.5275738897027722
1102	2	0.5673306605560526
1102	3	0.5836070361007728
1102	4	0.6903991376881891
1102	5	0.6137282992168451
1102	6	0.5686216744994042
1102	7	0.5430803191838862
1102	8	0.6253782654598297
1102	9	0.7455439542598441
1102	10	0.727246412062577
1102	11	0.6232923057571611
1102	12	0.695054825324803
1102	13	0.7342933765940458
1102	14	1.0486944920434098
1102	15	0.8599054442716749
1102	16	0.8867717592082016
1102	17	0.7780665482953353
1102	18	0.8036036227504866
1102	19	0.857950387400038
1102	20	0.7877425423667219
1102	21	0.777141012124865
1102	22	0.8285911856087438
1102	23	0.7544855669631829
1102	24	0.8747818543565842
1102	25	0.8931638985843335
1102	26	0.8980762635744881
1102	27	0.8535374552596622
1102	28	0.8507738785591812
1102	29	0.9771891874364727
1102	30	0.9181683692147757
1102	31	0.8969147709009988
1102	32	0.9090415691551215
1102	33	0.8701220072338032
1102	34	0.9970791974687501
1102	35	0.895720461611667
1102	36	0.8681553690947084
1102	37	0.9846832671180081
1102	38	0.8480507183811099
1102	39	0.9295231912154804
1102	40	0.9345272701508307
1102	41	0.9728490616160599
1102	42	0.9044726799374843
1102	43	0.8268389705799919
1102	44	0.9201524171038784
1102	45	0.9105666498217886
1102	46	0.8117063172298202
1103	1	0.5726525486175866
1103	2	0.5849230941887456
1103	3	0.6707882146948698
1103	4	0.6318599276010133
1103	5	0.6177854113132
1103	6	0.6128769575749189
1103	7	0.6090854976874738
1103	8	0.6947576206080726
1103	9	0.7508106737576767
1103	10	0.781727034322536
1103	11	0.6573213478127045
1103	12	0.7186096215895006
1103	13	0.7099939101363404
1103	14	0.8805571775817214
1103	15	0.7515987683767733
1103	16	0.7958850204045262
1103	17	0.8253158433468997
1103	18	0.7482510075176307
1103	19	0.6924459407769774
1103	20	0.7269385773074362
1103	21	0.7938058669219643
1103	22	0.8099386033174838
1103	23	0.8026736728019443
1103	24	0.8726888580605063
1103	25	0.7000935372122754
1103	26	0.6876476178674622
1103	27	0.7453807300179562
1103	28	0.7302445437350329
1103	29	0.8209237998677352
1103	30	0.7300697451370439
1103	31	0.8221326010748768
1103	32	0.7819926193262035
1103	33	0.7661274169352268
1103	34	0.7841782926325678
1103	35	0.8310356523097155
1103	36	0.7559826062658104
1103	37	0.7703431967453227
1103	38	0.7942784469377955
1103	39	0.8472507853986784
1103	40	0.8029370045307545
1103	41	0.900243676805303
1103	42	0.9458209371731172
1103	43	0.9386709215634284
1103	44	0.8733848661956785
1103	45	0.8522465857685049
1103	46	0.7200087462992215
1104	1	0.5894611052758307
1104	2	0.5568511872379212
1104	3	0.583197694466211
1104	4	0.645439017387595
1104	5	0.6449685978201103
1104	6	0.6875529391620248
1104	7	0.6915793125309406
1104	8	0.5998466452921889
1104	9	0.751975038812148
1104	10	0.7630169764038826
1104	11	0.7193330224783381
1104	12	0.7436333619382864
1104	13	0.7254132829104805
1104	14	0.9990287440485233
1104	15	0.8492889233742957
1104	16	0.7825070415753359
1104	17	0.7984784507711638
1104	18	0.7429236375260828
1104	19	0.6975994801024967
1104	20	0.8302849272369315
1104	21	0.7802837734583861
1104	22	0.8423165743523029
1104	23	0.9134268654079492
1104	24	0.8859203677063618
1104	25	0.7355827605228953
1104	26	0.7655445412351725
1104	27	0.8798767883406242
1104	28	0.841203962353049
1104	29	0.9016376568917295
1104	30	0.9186739880017853
1104	31	0.963093032960856
1104	32	0.9583693646965088
1104	33	0.9366318360176606
1104	34	0.9251300370448163
1104	35	0.8626232256173765
1104	36	0.8427129854034021
1104	37	0.8338066538207585
1104	38	0.820463548261344
1104	39	0.8689231094781462
1104	40	0.7692067916730352
1104	41	0.9169685251885866
1104	42	0.8443957368091191
1104	43	0.9145639938633394
1104	44	0.8791062577763284
1104	45	0.8399322947280723
1104	46	0.8529654979439982
1105	1	0.3397988437459283
1105	2	0.26995461224342776
1105	3	0.5146746652227066
1105	4	0.5355476412099662
1105	5	0.5594389564149651
1105	6	0.5961975478883446
1105	7	0.49863260326868897
1105	8	0.588595434081185
1105	9	0.767151464985254
1105	10	0.6194357899327727
1105	11	0.6400902187588287
1105	12	0.6905894820394849
1105	13	0.7316736999617888
1105	14	0.9722912212726698
1105	15	0.8210361931359742
1105	16	0.8424011871447519
1105	17	0.6700918347642073
1105	18	0.6267646820770025
1105	19	0.7082673682725442
1105	20	0.8014281830994463
1105	21	0.6555296763501417
1105	22	0.6185194771842148
1105	23	0.7363533867589993
1105	24	0.7169204619293481
1105	25	0.59647748416306
1105	26	0.7581760491467193
1105	27	0.732852006422263
1105	28	0.8778103878513193
1105	29	0.7823079070028243
1105	30	0.9485630468032937
1105	31	0.8604649438157281
1105	32	0.8869615523377661
1105	33	0.9399461931813491
1105	34	0.9177500854041298
1105	35	0.8471577640711772
1105	36	1.010336630788835
1105	37	0.9668630373643339
1105	38	0.9424789715453699
1105	39	0.8741952993094486
1105	40	0.7491250405006582
1105	41	0.704680479156842
1105	42	0.7365097822729822
1105	43	0.8701348323587794
1105	44	0.8078533085332325
1105	45	0.691509129115893
1105	46	0.7610390389838386
1106	1	0.026398986139234637
1106	2	0.16136670246986995
1106	3	0.19326018484558816
1106	4	0.2613514920506006
1106	5	0.19456055414022444
1106	6	0.14206098426478775
1106	7	0.16108857355083472
1106	8	0.17318407264437496
1106	9	0.10466295945155935
1106	10	0.17038996157098296
1106	11	0.16111322864097133
1106	12	0.07881692661925399
1106	13	0.13232738871338512
1106	14	0.41270266999917027
1106	15	0.2783629572052462
1106	16	0.3543886021920386
1106	17	0.24474833392670092
1106	18	0.23486964175074831
1106	19	0.19593761889555594
1106	20	0.12070835097499355
1106	21	0.09690956461297873
1106	22	0.12854573075158982
1106	23	0.1532970791894641
1106	24	0.18626273245821778
1106	25	0.16315840285699323
1106	26	0.1986419349038684
1106	27	0.25959153935571777
1106	28	0.23537470686945738
1106	29	0.27078217511043334
1106	30	0.30165813321850976
1106	31	0.3769761872051234
1106	32	0.38770113415344554
1106	33	0.43859605959636383
1106	34	0.3946460890912462
1106	35	0.2571590686554117
1106	36	0.11708177565640909
1106	37	0.08994881393635268
1106	38	-0.017508541266373356
1106	39	0.12219104845980411
1106	40	0.12026195550492957
1106	41	0.16634617611430258
1106	42	0.06432862853169752
1106	43	0.16297796578600554
1106	44	0.2684105850184366
1106	45	0.21063858735793417
1106	46	0.11519112430300993
1107	1	0.5463274402542453
1107	2	0.4532519356027507
1107	3	0.5457224740329458
1107	4	0.6015333193708372
1107	5	0.5525970101370703
1107	6	0.5324661934595483
1107	7	0.5371830448377324
1107	8	0.5637030722007665
1107	9	0.7149498578884348
1107	10	0.636538811851274
1107	11	0.625646536749997
1107	12	0.6903920443353044
1107	13	0.7021336391601949
1107	14	0.9190646282423245
1107	15	0.8908238915224516
1107	16	0.8862185291507956
1107	17	0.7435603250894545
1107	18	0.8237884584933184
1107	19	0.8075640905608168
1107	20	0.8272553395263387
1107	21	0.6669154554884855
1107	22	0.6732260079317953
1107	23	0.6763098920778319
1107	24	0.8065232234144588
1107	25	0.6275040895467043
1107	26	0.6074493401121259
1107	27	0.7454835636971779
1107	28	0.7119004888933276
1107	29	0.7976794693972877
1107	30	0.7485706370154261
1107	31	0.7712226405827565
1107	32	0.7907337955773315
1107	33	0.7410207152361394
1107	34	0.7045164430026816
1107	35	0.7265583585660949
1107	36	0.8583036062982643
1107	37	0.7702750067293778
1107	38	0.7397997188524599
1107	39	0.9378956168384462
1107	40	0.7748816724869201
1107	41	0.8705919145950176
1107	42	0.8008569579611944
1107	43	0.739609764838157
1107	44	0.6847236274542752
1107	45	0.6927930119611219
1107	46	0.7643011400713249
1108	1	0.20394807130314518
1108	2	0.17207002086244216
1108	3	0.24984203493234247
1108	4	0.2083701526432975
1108	5	0.2136134501038569
1108	6	0.35072331755952746
1108	7	0.37077155691437014
1108	8	0.3632691412560973
1108	9	0.3350961942562094
1108	10	0.5379793009404779
1108	11	0.42125644571675736
1108	12	0.329681513865971
1108	13	0.2770384618096955
1108	14	0.4838897634502075
1108	15	0.45321356928812406
1108	16	0.37755607496669086
1108	17	0.40441229676461177
1108	18	0.45706915485679644
1108	19	0.44460819048006783
1108	20	0.4674841159112262
1108	21	0.48229518509560165
1108	22	0.40104654000997897
1108	23	0.3774082535710548
1108	24	0.4977078907302541
1108	25	0.47654507242202726
1108	26	0.39357003101619625
1108	27	0.4159668808785071
1108	28	0.41349385648262427
1108	29	0.5601067377367954
1108	30	0.3474375061476138
1108	31	0.43357213532122074
1108	32	0.49037779073096743
1108	33	0.5206643901455337
1108	34	0.5922896704317537
1108	35	0.49748140158785503
1108	36	0.49842449087385177
1108	37	0.5212779430640566
1108	38	0.4166520077219147
1108	39	0.4654529386511186
1108	40	0.49918500783067543
1108	41	0.6022848095190696
1108	42	0.5466464705723411
1108	43	0.5790578315160602
1108	44	0.4685119490618419
1108	45	0.4007468113921533
1108	46	0.45335259950820017
1109	1	0.3165779575541059
1109	2	0.232286380983723
1109	3	0.3720707886732946
1109	4	0.33852858934483265
1109	5	0.34389979222028444
1109	6	0.21659219149383802
1109	7	0.3364189545108047
1109	8	0.29000673065783644
1109	9	0.3427085661432798
1109	10	0.34963001275863803
1109	11	0.32036080597085004
1109	12	0.3792891951332251
1109	13	0.37967995387352005
1109	14	0.5332335596225519
1109	15	0.32090466148717667
1109	16	0.3416845297334561
1109	17	0.33354522633437966
1109	18	0.32567520466972866
1109	19	0.35434854626086576
1109	20	0.2612667808412965
1109	21	0.27183953997690935
1109	22	0.26560957134567076
1109	23	0.27101539756010595
1109	24	0.2760768589900948
1109	25	0.3051995074573952
1109	26	0.36262069363416316
1109	27	0.31437836353996573
1109	28	0.17368969136919077
1109	29	0.2677646122581905
1109	30	0.3302818811013992
1109	31	0.37936672601635024
1109	32	0.3198890788554607
1109	33	0.21988948707444678
1109	34	0.358487702725796
1109	35	0.3006281011753913
1109	36	0.3456403031524715
1109	37	0.42629198624855036
1109	38	0.4093269136605002
1109	39	0.48871602839317774
1109	40	0.42084637466038544
1109	41	0.3594746785046823
1109	42	0.38299741586504155
1109	43	0.3762042956670797
1109	44	0.3418182776255776
1109	45	0.2848430747429944
1109	46	0.20518583499452347
1110	1	-0.05137677527220319
1110	2	-0.18631297656493473
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2216	12	-2.7805421294340817
2216	13	-2.776232546437967
2216	14	-4.640892398729125
2216	15	-3.4341442494044614
2216	16	-3.1578047646926706
2216	17	-3.0219608138077163
2216	18	-3.2255002903228913
2216	19	-3.102720759628099
2216	20	-2.8607022805624283
2216	21	-2.941008256194941
2216	22	-2.9406498598869746
2216	23	-2.9203143750935396
2216	24	-2.8800636521861804
2216	25	-2.8562454750576194
2216	26	-2.901624655470272
2216	27	-3.060923782004892
2216	28	-3.016338190469405
2216	29	-3.2691235862760095
2216	30	-2.9625034508918375
2216	31	-3.1100081338130394
2216	32	-3.123602297709951
2216	33	-2.992433759512309
2216	34	-3.045158671389224
2216	35	-2.9630233398477372
2216	36	-3.1574235149704535
2216	37	-3.166156105631387
2216	38	-3.122975594597456
2216	39	-3.099574430037407
2216	40	-3.0894189596586443
2216	41	-3.2685078060523196
2216	42	-3.1316265669462346
2216	43	-3.0548493443387947
2216	44	-3.1414974738135086
2216	45	-3.2039272171164512
2216	46	-3.3056727152052474
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	93069.0
3	2981.0
4	8765.0
5	6917.0
6	5314.0
7	4333.0
8	3646.0
9	3362.0
10	3004.0
11	3026.0
12	2904.0
13	2941.0
14	3243.0
15	3293.0
16	3497.0
17	3935.0
18	4292.0
19	5421.0
20	7334.0
21	10201.0
22	13343.0
23	15292.0
24	15936.0
25	16740.0
26	18085.0
27	20975.0
28	23905.0
29	26111.0
30	28873.0
31	34994.0
32	43434.0
33	53885.0
34	68643.0
35	91788.0
36	126597.0
37	184943.0
38	325401.0
39	1075521.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.29073976278432	18.714053337319818	14.147352889983573	23.84785400991229
2	15.101204165056123	20.616875565099935	39.44310855078139	24.83881171906255
3	17.97443449516079	26.013645537455865	29.824744674840005	26.187175292543337
4	12.383310172282693	17.395527873189653	38.907647037429555	31.3135149170981
5	13.62548726030218	37.93583872196681	34.762658408488086	13.676015609242926
6	30.60981155936066	37.94937665473063	18.189230176200283	13.25158160970843
7	27.222706877254915	32.72042787149654	22.461689710322812	17.595175540925737
8	26.00340498338084	34.53116388215444	20.714649205560235	18.750781928904487
9	26.42708365033154	16.184026333674847	19.724262281778433	37.66462773421518
10	14.291335720541145	29.240590647961234	34.1846214449708	22.283452186526816
11	33.4481712162249	24.060079190378133	24.312240695468702	18.17950889792827
12	23.05697007198818	25.405292771088412	30.623717213932366	20.914019942991043
13	27.160575229168572	23.008152348491766	26.34495998214666	23.486312440193004
14	21.991940637648227	21.908253111654375	27.697908319047283	28.401897931650115
15	23.106938755498334	29.428054691089343	24.340062292286838	23.124944261125478
16	22.806879621833822	28.93631463804722	25.7626131472258	22.494192592893153
17	21.77690201518383	28.49292754078932	27.02150267673963	22.708667767287224
18	21.71575877024101	28.37298023142726	27.93539276571086	21.975868232620872
19	22.783508422674334	27.850046598913323	28.113156109529637	21.253288868882702
20	23.093062731625505	27.228845997781804	27.943672735142155	21.734418535450537
21	23.19023248815242	27.07791252656512	27.899465907880522	21.832389077401935
22	22.93562315929709	26.844845017464486	27.788062608413387	22.431469214825032
23	22.775617957867027	26.977545480904404	27.638002348328655	22.608834212899914
24	22.379754625342308	27.165181769728896	27.837939620437265	22.61712398449153
25	22.743136199891207	26.986251055083272	27.77837186016306	22.492240884862465
26	22.57403655059372	27.18614191652655	27.832442772935416	22.40737875994431
27	22.623986944959984	27.31513463010015	27.56088899411086	22.499989430829004
28	22.57484371896041	27.164539948350118	27.654283939085268	22.606332393604205
29	22.714387094401793	27.339017100754504	27.587884454201518	22.35871135064218
30	22.879328191695976	27.233588048250297	27.55616964782729	22.330914112226445
31	22.875708977571815	26.959385977555332	27.649818864393232	22.51508618047962
32	22.73528419739248	27.216235841695806	27.52710777936419	22.521372181547523
33	22.74381460554479	27.102417012413056	27.476271146322578	22.677497235719578
34	22.908766139899132	27.062787736973537	27.635852428445105	22.392593694682223
35	22.92086523115211	27.11780946519869	27.437731625503826	22.523593678145374
36	22.79508573081109	27.226815221077	27.470516160619386	22.50758288749253
37	22.94078162038963	27.012721536323337	27.517550444847984	22.52894639843906
38	22.74711529560162	27.095909948441605	27.57564779827423	22.58132695768255
39	22.87533110734475	27.268867621338366	27.417548773845652	22.438252497471236
40	23.009946042465984	26.989842591449648	27.661056728966116	22.339154637118252
41	22.906048113569813	27.077712161016876	27.421156978937418	22.595082746475896
42	22.80366393282818	27.152797366786167	27.416355580291157	22.627183120094497
43	22.831325708700234	27.030933710337223	27.429273720720616	22.70846686024193
44	22.868088251210867	27.13432164407284	27.323815515328626	22.673774589387666
45	22.95339030703056	27.18680320737056	27.155433006017837	22.704373479581044
46	22.944535714799073	26.98038114838305	27.160056447637544	22.915026689180333
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	907.0
1	964.5
2	1022.0
3	1204.0
4	1386.0
5	1454.5
6	1523.0
7	1523.0
8	1475.0
9	1427.0
10	1631.0
11	1835.0
12	2631.5
13	3428.0
14	6151.0
15	8874.0
16	12263.5
17	15653.0
18	16537.0
19	17421.0
20	17421.0
21	19154.5
22	20888.0
23	25534.5
24	30181.0
25	37027.0
26	43873.0
27	50605.0
28	57337.0
29	64573.0
30	71809.0
31	71809.0
32	79801.0
33	87793.0
34	96479.5
35	105166.0
36	113980.5
37	122795.0
38	129459.0
39	136123.0
40	143527.0
41	150931.0
42	156451.5
43	161972.0
44	161972.0
45	165829.0
46	169686.0
47	172299.5
48	174913.0
49	175733.0
50	176553.0
51	171762.0
52	166971.0
53	159448.0
54	151925.0
55	141962.5
56	132000.0
57	132000.0
58	120680.0
59	109360.0
60	96683.0
61	84006.0
62	72275.0
63	60544.0
64	50118.5
65	39693.0
66	32131.0
67	24569.0
68	19699.5
69	14830.0
70	14830.0
71	11640.0
72	8450.0
73	6602.0
74	4754.0
75	3648.5
76	2543.0
77	1932.5
78	1322.0
79	1033.5
80	745.0
81	745.0
82	554.5
83	364.0
84	272.5
85	181.0
86	141.0
87	101.0
88	64.0
89	27.0
90	21.0
91	15.0
92	12.0
93	9.0
94	9.0
95	10.0
96	11.0
97	10.5
98	10.0
99	14.0
100	18.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.19582035754016155
2	0.0557071511413626
3	0.0637377723225909
4	0.056721545395833545
5	0.039519109497097145
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	4.2266427269622615E-5
16	0.0
17	2.5359856361773566E-4
18	7.185292635835844E-4
19	3.803978454266035E-4
20	0.0023669199270988663
21	0.0025359856361773566
22	0.0
23	2.5359856361773566E-4
24	4.649306999658487E-4
25	7.185292635835844E-4
26	7.60795690853207E-4
27	0.024260929252763377
28	5.917299817747166E-4
29	0.0010566606817405653
30	0.001310259245358301
31	0.0015638578089760366
32	7.60795690853207E-4
33	0.0020287885089418853
34	0.0028741170543343376
35	0.0023669199270988663
36	0.031699820452216955
37	0.008115154035767541
38	0.011707800353685464
39	0.04869092421460525
40	0.016061242362456592
41	0.05798953821392223
42	0.05836993605934883
43	0.026543316325323
44	0.029417433379657337
45	0.027135046307097718
46	0.023922797834606397
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
46	2365944.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	71.58547726791318
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.28226997323647	59.618010439893276
2	10.514165456593918	15.053231045681637
3	2.847242406935769	6.114636197938166
4	1.2128723253984963	3.472961771147802
5	0.6197360445149869	2.218205026336701
6	0.36935171909902936	1.5864131454856933
7	0.251304452015554	1.2592824395959374
8	0.1712740647687779	0.9808588536070753
9	0.12594345492041248	0.8114150096322874
>10	0.5843497724131622	7.029430136324664
>50	0.016909820353033105	0.7918404538746805
>100	0.004343402275712256	0.4667167490642267
>500	5.927686868355383E-5	0.0288124343331288
>1k	1.185537373671042E-4	0.271320626106068
>5k	5.92768686835521E-5	0.2968656709787381
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6996	0.2956959251782798	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3200	0.13525256726279236	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3194	0.13499896869917463	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	4.2266427269622615E-5	0.0
10	0.0	0.0	0.0	8.453285453924523E-5	0.0
11	0.0	0.0	0.0	8.453285453924523E-5	0.0
12	0.0	0.0	0.0	8.453285453924523E-5	0.0
13	0.0	0.0	0.0	8.453285453924523E-5	4.2266427269622615E-5
14	0.0	0.0	0.0	8.453285453924523E-5	4.2266427269622615E-5
15	0.0	0.0	0.0	8.453285453924523E-5	8.453285453924523E-5
16	0.0	0.0	0.0	8.453285453924523E-5	8.453285453924523E-5
17	0.0	0.0	0.0	8.453285453924523E-5	8.453285453924523E-5
18	0.0	0.0	0.0	8.453285453924523E-5	8.453285453924523E-5
19	0.0	0.0	0.0	8.453285453924523E-5	8.453285453924523E-5
20	0.0	0.0	0.0	8.453285453924523E-5	8.453285453924523E-5
21	0.0	4.2266427269622615E-5	0.0	8.453285453924523E-5	8.453285453924523E-5
22	0.0	4.2266427269622615E-5	0.0	2.1133213634811306E-4	8.453285453924523E-5
23	0.0	8.453285453924523E-5	0.0	2.5359856361773566E-4	1.2679928180886783E-4
24	0.0	8.453285453924523E-5	0.0	2.958649908873583E-4	1.6906570907849046E-4
25	0.0	8.453285453924523E-5	0.0	2.958649908873583E-4	1.6906570907849046E-4
26	0.0	8.453285453924523E-5	0.0	2.958649908873583E-4	2.1133213634811306E-4
27	0.0	8.453285453924523E-5	0.0	3.803978454266035E-4	2.5359856361773566E-4
28	0.0	8.453285453924523E-5	0.0	5.49463554505094E-4	2.5359856361773566E-4
29	0.0	8.453285453924523E-5	0.0	0.0011411935362798105	2.5359856361773566E-4
30	0.0	8.453285453924523E-5	4.2266427269622615E-5	0.0018174563725937723	2.5359856361773566E-4
31	0.0	8.453285453924523E-5	4.2266427269622615E-5	0.0036349127451875446	2.5359856361773566E-4
32	0.0	8.453285453924523E-5	4.2266427269622615E-5	0.005452369117781317	2.5359856361773566E-4
33	0.0	8.453285453924523E-5	4.2266427269622615E-5	0.00760795690853207	2.5359856361773566E-4
34	0.0	8.453285453924523E-5	4.2266427269622615E-5	0.009509946135665087	2.5359856361773566E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1565	0.0	23.691496	1
GTATCAA	3895	0.0	23.463484	1
TATCAAC	4985	0.0	17.988174	2
TCAACGC	5055	0.0	17.4904	4
ATCAACG	5075	0.0	17.424788	3
CAACGCA	5120	0.0	17.188036	5
AACGCAG	5340	0.0	16.510736	6
GCGCAAT	75	0.0024489786	15.994074	11
ACGCCCG	80	0.0037562903	14.999517	21
GCGTGCG	155	7.888302E-7	14.188291	9
TCGCGTC	85	0.0056271497	14.11391	17
ACGCAGA	6275	0.0	13.923129	7
GTATTAC	305	0.0	13.799219	1
CGCAGAG	6340	0.0	13.591178	8
GGACCGT	195	5.3343683E-8	13.328395	6
GAGTACT	3225	0.0	13.204688	12
GTGTAGC	705	0.0	12.79259	1
AGTACTT	3415	0.0	12.5871105	13
AGAGTAC	6325	0.0	12.580319	11
TAGCCCT	850	0.0	12.47256	4
>>END_MODULE