FastQCFastQC Report
Mon 6 Feb 2017
SRR2077326_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077326_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2365944
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23700.042.5811161
GTATCAA53550.038.208941
ATCAACG74150.027.2555163
TCAACGC74400.027.2105274
TATCAAC74450.027.1945672
CAACGCA75700.026.6974475
AACGCAG77800.026.2436076
ACGCAGA89550.022.5775017
CGCAGAG92200.021.9188468
AGAGTAC93250.020.52773511
GAGTACT68450.020.25580412
CAGAGTA100100.019.4318610
GCAGAGT102000.019.204339
GTACATG60150.018.9684871
AGTACTT73150.018.95433813
GTACTTT73550.018.84727114
CGTATAC1301.1391282E-418.666023
TACATGG61400.018.294082
GTATAGA5950.017.4854451
TACTTTT81450.017.23128315
Produced by FastQC (version 0.11.5)