FastQCFastQC Report
Mon 6 Feb 2017
SRR2077325_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077325_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2316275
Sequences flagged as poor quality0
Sequence length46
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54680.23606868787169055No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT24980.1078455710138045No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24530.10590279651595774No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14050.024.972791
GTATCAA33700.022.8458181
TATCAAC44600.016.9662532
TCAACGC45250.016.7979474
ATCAACG45200.016.7319953
CAACGCA46200.016.5359135
AACGCAG48850.015.6754186
CGTATAC800.0037460115.0056783
TAAACCG800.003756789714.9991995
TTATACC3650.013.7005614
ACGCAGA56000.013.352687
CGCAGAG57800.012.79849158
GTATAGG4000.012.530991
GAGTACT28950.012.5001412
GTACATG62050.012.4724241
ACCGTGC3109.094947E-1212.253978
TTATACT6450.012.0947274
GTATTGG5500.012.0297491
GTATTAA6400.011.904441
AGAGTAC58500.011.85928411
Produced by FastQC (version 0.11.5)