FastQCFastQC Report
Mon 6 Feb 2017
SRR2077325_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077325_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2316275
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20400.040.9637341
GTATCAA46400.040.1299821
TATCAAC65600.028.164632
ATCAACG65900.027.9281833
TCAACGC66850.027.3239064
CAACGCA66950.027.0754245
AACGCAG69250.026.4758956
CGTATAC750.001940571923.1069363
ACGCAGA81200.022.5659627
CGCAGAG82300.022.1261358
AGAGTAC83250.020.63082311
GAGTACT57850.020.32930612
AGTACTT60600.019.8620613
CAGAGTA89650.019.69659410
GTACTTT61250.019.42227414
GCAGAGT93300.019.1847979
GTACATG59050.019.0255761
TACATGG58950.018.5228372
ACATGGG60900.017.8708823
GTGTACG2208.101233E-817.3373491
Produced by FastQC (version 0.11.5)