FastQCFastQC Report
Mon 6 Feb 2017
SRR2077324_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077324_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1594840
Sequences flagged as poor quality0
Sequence length46
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33500.21005241905144087No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16310.10226731208146271No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10800.024.6914881
GTATCAA24250.022.4066491
GTATAGT2500.019.248221
CCGTCGT550.007793557418.1749069
TATCAAC32550.016.6695382
TCAACGC32500.016.3127444
ATCAACG32400.016.3028813
CAACGCA33150.016.0502175
TACCGTC750.00244866815.9939177
AACGCAG34300.015.7957436
ATACCGT1058.5578E-515.2323036
GTGTACG1201.5452124E-515.0376731
GTATTAA5100.014.9393861
ACAGCGT2054.456524E-1014.6285838
GTATAGC2900.014.5191321
GTCCTAA2650.014.3756361
TCTATAC2900.013.7986763
TAATACG900.00818240713.3374654
TACTCCG1202.650225E-413.3349545
GCCCCGT900.00821980713.3282656
Produced by FastQC (version 0.11.5)