##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2077324_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1594840
Sequences flagged as poor quality	0
Sequence length	46
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.283253492513357	31.0	31.0	34.0	26.0	34.0
2	30.422846805949185	31.0	31.0	34.0	26.0	34.0
3	30.579216096912543	33.0	31.0	34.0	26.0	34.0
4	33.97872827368263	37.0	35.0	37.0	30.0	37.0
5	34.06312670863535	37.0	35.0	37.0	30.0	37.0
6	34.10000877831005	37.0	35.0	37.0	30.0	37.0
7	33.99136653206591	37.0	35.0	37.0	30.0	37.0
8	33.97304996112463	37.0	35.0	37.0	30.0	37.0
9	35.48698678237315	39.0	37.0	39.0	30.0	39.0
10	35.42161596147576	39.0	37.0	39.0	29.0	39.0
11	35.458814050312256	39.0	37.0	39.0	30.0	39.0
12	35.38194677836021	39.0	37.0	39.0	29.0	39.0
13	35.350727972711994	39.0	37.0	39.0	29.0	39.0
14	36.44366456823255	40.0	37.0	41.0	27.0	41.0
15	36.57619886634396	40.0	37.0	41.0	29.0	41.0
16	36.56073085701387	40.0	37.0	41.0	28.0	41.0
17	36.58375511023049	40.0	37.0	41.0	29.0	41.0
18	36.54731007499185	40.0	37.0	41.0	28.0	41.0
19	36.63415138822703	40.0	38.0	41.0	29.0	41.0
20	36.62241165257957	40.0	38.0	41.0	29.0	41.0
21	36.63566063053347	40.0	38.0	41.0	29.0	41.0
22	36.589101101050886	40.0	37.0	41.0	29.0	41.0
23	36.548125830804345	40.0	37.0	41.0	29.0	41.0
24	36.57679265631662	40.0	37.0	41.0	29.0	41.0
25	36.51951606430739	40.0	37.0	41.0	29.0	41.0
26	36.54847382809561	40.0	37.0	41.0	29.0	41.0
27	36.51810714554438	40.0	37.0	41.0	29.0	41.0
28	36.464193273306414	40.0	37.0	41.0	29.0	41.0
29	36.42409081788769	40.0	37.0	41.0	29.0	41.0
30	36.34152140653608	40.0	37.0	41.0	28.0	41.0
31	36.308089212710996	40.0	37.0	41.0	28.0	41.0
32	36.2786887712874	40.0	37.0	41.0	29.0	41.0
33	36.22630483308671	40.0	37.0	41.0	28.0	41.0
34	36.18238820195129	40.0	37.0	41.0	28.0	41.0
35	36.15526447794136	40.0	37.0	41.0	28.0	41.0
36	36.105073236186705	40.0	37.0	41.0	28.0	41.0
37	36.01545233377643	40.0	36.0	41.0	27.0	41.0
38	35.978417270698	40.0	36.0	41.0	27.0	41.0
39	35.89870143713476	40.0	36.0	41.0	27.0	41.0
40	35.87272516365278	40.0	36.0	41.0	27.0	41.0
41	35.74433171979634	40.0	36.0	41.0	27.0	41.0
42	35.69458817185423	40.0	36.0	41.0	26.0	41.0
43	35.600404429284445	40.0	36.0	41.0	26.0	41.0
44	35.52014057836523	40.0	36.0	41.0	26.0	41.0
45	35.42753505053798	40.0	36.0	41.0	25.0	41.0
46	35.32337287753003	40.0	35.0	41.0	25.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.37077931617042026
1101	2	0.4556925800876108
1101	3	0.5622892801066754
1101	4	0.5591525239439292
1101	5	0.5575514657367293
1101	6	0.5000704685637203
1101	7	0.5914333158807992
1101	8	0.6663952220865994
1101	9	0.6173973033609741
1101	10	0.6433142902055877
1101	11	0.6248606157442467
1101	12	0.661868344789049
1101	13	0.7172205282673687
1101	14	0.9032257601498515
1101	15	0.8174460514585249
1101	16	0.5731097280888804
1101	17	0.7129635772740031
1101	18	0.558910787627255
1101	19	0.6791782882029835
1101	20	0.4355615426838213
1101	21	0.5949980630239224
1101	22	0.729327397838496
1101	23	0.6716359815878192
1101	24	0.6467173654376666
1101	25	0.7110613927890341
1101	26	0.7207355383660072
1101	27	0.6942289366440662
1101	28	0.5286328394876136
1101	29	0.6749013412811138
1101	30	0.7784772378264577
1101	31	0.7809387230341969
1101	32	0.6696956120206679
1101	33	0.7438644448304004
1101	34	0.6259957869258912
1101	35	0.5950702353874817
1101	36	0.5537773292090336
1101	37	0.5985566087882432
1101	38	0.3733368471096483
1101	39	0.5285652385505344
1101	40	0.5346974071467017
1101	41	0.5204677940581419
1101	42	0.5180301546334718
1101	43	0.46677556733757086
1101	44	0.5776571714981316
1101	45	0.5752259698296172
1101	46	0.498017930609052
1102	1	0.6339918591709122
1102	2	0.6279858522355752
1102	3	0.736332050906384
1102	4	0.6833360161774351
1102	5	0.726363960260592
1102	6	0.7535665998502239
1102	7	0.8058764856300726
1102	8	0.7067442374459958
1102	9	0.9155832374409414
1102	10	0.9090348027804396
1102	11	0.9510084350738097
1102	12	0.8847705636594014
1102	13	0.8278397762736418
1102	14	1.1227000179947524
1102	15	1.085601040743711
1102	16	1.1307872962652823
1102	17	0.9989410978745212
1102	18	0.9654035034403847
1102	19	0.9497857691623963
1102	20	1.0698522485182949
1102	21	0.9901454459000405
1102	22	0.9610618621840104
1102	23	1.0893812942170413
1102	24	1.0366709113967048
1102	25	1.0639868182083134
1102	26	1.1281220420463782
1102	27	1.179698435263056
1102	28	1.2356045076814581
1102	29	1.2071581576930726
1102	30	1.1640717766720456
1102	31	1.1289653065699952
1102	32	1.1224325354087625
1102	33	1.1139517846507587
1102	34	1.1678963410497971
1102	35	1.1598393531537567
1102	36	1.1627164771176197
1102	37	1.2393582910583234
1102	38	1.2092866492316503
1102	39	1.2328858410364418
1102	40	1.2606412888964798
1102	41	1.2660187121252662
1102	42	1.24227571660618
1102	43	1.2165030910088248
1102	44	1.1846926699844644
1102	45	1.3317295765072785
1102	46	1.2214303005021137
1103	1	0.3967357882721636
1103	2	0.4364080605623286
1103	3	0.5410436639119993
1103	4	0.6135786889560322
1103	5	0.5424830023434666
1103	6	0.4951411766584002
1103	7	0.5252447947528509
1103	8	0.6767051598383773
1103	9	0.7050812542285598
1103	10	0.7310054820454894
1103	11	0.6720669279411808
1103	12	0.6755471449116683
1103	13	0.6643566030328216
1103	14	0.8677365479917754
1103	15	0.8667866402461186
1103	16	0.8865816777689517
1103	17	0.8380061179863674
1103	18	0.8771955702411987
1103	19	0.8361728260961954
1103	20	0.8657205544359172
1103	21	0.915283767655005
1103	22	0.8052182184578882
1103	23	0.6239577993615057
1103	24	0.768438110099261
1103	25	0.7249013045457176
1103	26	0.5948530448647773
1103	27	0.71251540881822
1103	28	0.6928659360411515
1103	29	0.8650957372512735
1103	30	0.8263382546037903
1103	31	0.8079753068399995
1103	32	0.7259784830179186
1103	33	0.8469559779614713
1103	34	0.7980491335826088
1103	35	0.7949669308490783
1103	36	0.8974706836860094
1103	37	0.8037790393895534
1103	38	0.8752913062210865
1103	39	0.8856314620696963
1103	40	0.811587964677436
1103	41	0.8003555107140699
1103	42	0.8410312238837179
1103	43	0.8191273144635076
1103	44	0.8435480886638231
1103	45	0.8045898021383593
1103	46	0.8062944826511256
1104	1	0.4351916325028746
1104	2	0.39132769699136105
1104	3	0.524489835095725
1104	4	0.5342173762792157
1104	5	0.5837410711164281
1104	6	0.47367679934655627
1104	7	0.5346389791266191
1104	8	0.5471617916261522
1104	9	0.5919220223341881
1104	10	0.6330999969365863
1104	11	0.6367613119430473
1104	12	0.5431090448470997
1104	13	0.6711670946258579
1104	14	0.9081059337030197
1104	15	0.8093656482881215
1104	16	0.7932207339722197
1104	17	0.6765806703553707
1104	18	0.618044289378858
1104	19	0.6113166673023258
1104	20	0.7649833497003868
1104	21	0.6595506606294137
1104	22	0.6809299549541663
1104	23	0.7194192145241658
1104	24	0.6636819128277125
1104	25	0.5224991406024628
1104	26	0.6717773740766475
1104	27	0.7204675900084325
1104	28	0.6672297280287935
1104	29	0.7172043197654006
1104	30	0.7192416032343516
1104	31	0.7852577714952176
1104	32	0.7760990585397991
1104	33	0.765805041463814
1104	34	0.7420023444194115
1104	35	0.6388597916788257
1104	36	0.8235152070904519
1104	37	0.7239559562683837
1104	38	0.7394873190602596
1104	39	0.7131610629724747
1104	40	0.7299501677610465
1104	41	0.8589505602514436
1104	42	0.6778044693140401
1104	43	0.7528141291457047
1104	44	0.7664971604209612
1104	45	0.6604019330068951
1104	46	0.7538298140274406
1105	1	0.13240146773627615
1105	2	0.06626251320770393
1105	3	0.25610078332355357
1105	4	0.2304721031201069
1105	5	0.3403594048853904
1105	6	0.33195346606743925
1105	7	0.22625774222153439
1105	8	0.28542842687195247
1105	9	0.5088652291304712
1105	10	0.2650515311432713
1105	11	0.45061692754539706
1105	12	0.41571366136824395
1105	13	0.41675591233028086
1105	14	0.6127001752883245
1105	15	0.4502282492317349
1105	16	0.46351939731221137
1105	17	0.3320813949138355
1105	18	0.33921013440013326
1105	19	0.3759166811466699
1105	20	0.521332361815702
1105	21	0.370443889345907
1105	22	0.3252001178597226
1105	23	0.4245036820408359
1105	24	0.32708336966116036
1105	25	0.41540501154972986
1105	26	0.42129461879508767
1105	27	0.5387649324618877
1105	28	0.5968677438393328
1105	29	0.47353347319136674
1105	30	0.6148594146252151
1105	31	0.5019864287943818
1105	32	0.5809490422178456
1105	33	0.600949361178877
1105	34	0.5721776417985538
1105	35	0.6346094647669744
1105	36	0.7887067072688012
1105	37	0.7493796177643546
1105	38	0.6021192800509851
1105	39	0.6863618562609233
1105	40	0.42463038423210975
1105	41	0.474697403949186
1105	42	0.545964711158831
1105	43	0.7080159520599096
1105	44	0.570998576818333
1105	45	0.4626400262095913
1105	46	0.4547783785089834
1106	1	0.04297005803436704
1106	2	0.12260370792880693
1106	3	0.18561237085113547
1106	4	0.27596677199866093
1106	5	0.3450405603689717
1106	6	0.27098124082502295
1106	7	0.3271316488247251
1106	8	0.3089882648356408
1106	9	0.18169609903076633
1106	10	0.4683381069781021
1106	11	0.471921142207016
1106	12	0.41117016524795247
1106	13	0.4176775113608002
1106	14	0.5849357375484985
1106	15	0.5378042981979263
1106	16	0.44733179874386764
1106	17	0.37127155980653015
1106	18	0.4392658247074763
1106	19	0.45869731920058143
1106	20	0.35977444850313844
1106	21	0.3478454555298427
1106	22	0.31494053321137727
1106	23	0.47429582930207914
1106	24	0.5216067383977077
1106	25	0.4912044586271662
1106	26	0.39129962023434217
1106	27	0.43447055003274926
1106	28	0.5656105913573555
1106	29	0.49803554987592236
1106	30	0.5725443637575935
1106	31	0.5684714784715368
1106	32	0.447416879867518
1106	33	0.5266588228920668
1106	34	0.4951775913050298
1106	35	0.38639046733256066
1106	36	0.34343432048395073
1106	37	0.45563591333061737
1106	38	0.3349230087584729
1106	39	0.4017068026143136
1106	40	0.39057249948340456
1106	41	0.47175256241593644
1106	42	0.2829711968129587
1106	43	0.3657708181433321
1106	44	0.31466965534166746
1106	45	0.40275359610571826
1106	46	0.1985915391863955
1107	1	0.18408961395007495
1107	2	0.18404971376724077
1107	3	0.13969164668095502
1107	4	0.22942977656411045
1107	5	0.2239588677056119
1107	6	0.22895913311344174
1107	7	0.15528279245673104
1107	8	0.21782446653267584
1107	9	0.3059859856538836
1107	10	0.27172220611510767
1107	11	0.25976009140242695
1107	12	0.2235052763186971
1107	13	0.13115306132170446
1107	14	0.33706660986504033
1107	15	0.28462803539175496
1107	16	0.30304768811164706
1107	17	0.2448355448712647
1107	18	0.3258440610008506
1107	19	0.28051101582056504
1107	20	0.2790306725945513
1107	21	0.24231547643410067
1107	22	0.18020170876034314
1107	23	0.20784738250434742
1107	24	0.39410865781482585
1107	25	0.2288382324211966
1107	26	0.0754249407876415
1107	27	0.21134576024618212
1107	28	0.20546283526595488
1107	29	0.33994511801061833
1107	30	0.1998955610339408
1107	31	0.3322394823203112
1107	32	0.23850553352235693
1107	33	0.2870270458595101
1107	34	0.11975201998573226
1107	35	0.06498176417733248
1107	36	0.09324521709655187
1107	37	0.11981401962871985
1107	38	0.10720048764488155
1107	39	0.2651576591497715
1107	40	0.1820033486675996
1107	41	0.3928606160205419
1107	42	0.30215596607843764
1107	43	0.2507606569406775
1107	44	0.3068800504222864
1107	45	0.33393007462456126
1107	46	0.19136285819848808
1108	1	0.11454631457272413
1108	2	0.11983082969428338
1108	3	0.050275297205697456
1108	4	-0.02756676916646228
1108	5	0.06437216994319073
1108	6	-0.04252691779853279
1108	7	-0.0030236804481589274
1108	8	0.10903285578704214
1108	9	0.03872976466864486
1108	10	0.1744526190873188
1108	11	0.022107179475291616
1108	12	-0.1358872562988438
1108	13	-0.18063050779668544
1108	14	-0.013445480077969307
1108	15	0.15782114445426743
1108	16	0.12201481754773624
1108	17	0.03662230423849877
1108	18	0.19982373141514387
1108	19	0.049692386967514324
1108	20	0.022604796376207048
1108	21	0.047659418261567055
1108	22	0.208555045320594
1108	23	0.021829748128865845
1108	24	0.16022766509739483
1108	25	0.15418915979353187
1108	26	0.06701891011724825
1108	27	0.01013319066575491
1108	28	0.10048577205503051
1108	29	0.17915932671889578
1108	30	0.0427345524752738
1108	31	0.15037879718686753
1108	32	0.0947036919787152
1108	33	0.062103501823074225
1108	34	0.21299254274650536
1108	35	0.10551167551042084
1108	36	0.10281733872204057
1108	37	0.13038859444555584
1108	38	0.002860099094576185
1108	39	0.12499572651902469
1108	40	0.1568595124483778
1108	41	0.21099168024354498
1108	42	0.21632452122574364
1108	43	0.19521732456075114
1108	44	0.11526022907622036
1108	45	0.11524539221933594
1108	46	0.1042731902043812
1109	1	0.1499934975564443
1109	2	0.023115853454871882
1109	3	0.20574064045649365
1109	4	0.21561089696736246
1109	5	0.17881719435624177
1109	6	0.1508860434124415
1109	7	0.2053076139330372
1109	8	0.11419480295914752
1109	9	0.15474682118296101
1109	10	0.1305023260219329
1109	11	0.043999408620891245
1109	12	0.08618045866108304
1109	13	0.25022454717346676
1109	14	0.36542037318272236
1109	15	0.2128796487877196
1109	16	0.3306281669752025
1109	17	0.24333670957130948
1109	18	0.2383531303864217
1109	19	0.3150749215753237
1109	20	0.20376909242118302
1109	21	0.12305782951005284
1109	22	0.18153824979215472
1109	23	0.1945201590728729
1109	24	0.27128531035234715
1109	25	0.21437494911268828
1109	26	0.24636853875610143
1109	27	0.2842302067272584
1109	28	0.27784130549927966
1109	29	0.26867884085019256
1109	30	0.32901974478923535
1109	31	0.3811664308061964
1109	32	0.244873196866763
1109	33	0.17271937411398852
1109	34	0.32929301360852037
1109	35	0.33744520023338964
1109	36	0.24900952763231743
1109	37	0.3586968622605582
1109	38	0.364670797340537
1109	39	0.39992395540363646
1109	40	0.38536284001826715
1109	41	0.33707912138579843
1109	42	0.3328248093976427
1109	43	0.3442851156415614
1109	44	0.3461504899533523
1109	45	0.34205910811446216
1109	46	0.3677051272632852
1110	1	0.2811339054271933
1110	2	0.18782841837830233
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2216	16	-2.3757813376409302
2216	17	-2.2888438720121016
2216	18	-2.534868080551931
2216	19	-2.308124005007585
2216	20	-2.1251909729541083
2216	21	-2.208792578585083
2216	22	-2.2283617149414567
2216	23	-2.292219324742561
2216	24	-2.1855578119066337
2216	25	-2.2761892307141096
2216	26	-2.2694440807242557
2216	27	-2.3235845664168266
2216	28	-2.332318035001549
2216	29	-2.615804153272272
2216	30	-2.341117334906329
2216	31	-2.3694471985057675
2216	32	-2.508268396809072
2216	33	-2.3416132639003564
2216	34	-2.2721658610109188
2216	35	-2.224744783758375
2216	36	-2.445742786319812
2216	37	-2.3951172361709396
2216	38	-2.5606872095137447
2216	39	-2.4812343424130887
2216	40	-2.4707754548664553
2216	41	-2.5929599248084685
2216	42	-2.4083362202396614
2216	43	-2.5178129932203177
2216	44	-2.4784049562901345
2216	45	-2.5248174789467157
2216	46	-2.6178739215568214
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	50944.0
3	1598.0
4	4821.0
5	3778.0
6	2971.0
7	2460.0
8	2094.0
9	2020.0
10	1889.0
11	1768.0
12	1847.0
13	1757.0
14	1878.0
15	1972.0
16	2102.0
17	2344.0
18	2636.0
19	3300.0
20	4373.0
21	6055.0
22	7824.0
23	9220.0
24	9820.0
25	10388.0
26	11288.0
27	13002.0
28	14849.0
29	15990.0
30	18149.0
31	21862.0
32	27465.0
33	34604.0
34	44664.0
35	59878.0
36	82926.0
37	122279.0
38	223716.0
39	764309.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.615007635995156	18.759042419193833	13.8563000572307	23.76964988758031
2	15.095367026922585	20.595626742414925	39.510128904201366	24.79887732646112
3	17.91119878131721	25.919596047813727	30.08303154872598	26.086173622143082
4	12.409808698139805	17.183038859503892	38.964085338961205	31.4430671033951
5	13.49249012849423	38.12179509294427	34.98273466584286	13.402980112718641
6	30.73261267587971	38.08294248952873	18.080183592084474	13.104261242507084
7	27.28888164329964	32.72848687015625	22.53022246745755	17.452409019086552
8	26.16575957462818	34.63532391964084	20.605515286799932	18.593401218931053
9	26.486983020240274	15.955769857791378	19.764929397306314	37.792317724662034
10	14.287828246093651	29.12198088836498	34.51324270773244	22.076948157808935
11	33.71052895588272	23.73705199267638	24.512364876727446	18.04005417471345
12	22.913144892277597	25.332447142033054	30.828923277570162	20.925484688119184
13	27.220034611622484	22.794073386672018	26.561786762308444	23.424105239397054
14	21.997253642998672	21.781432620200146	27.705036241880066	28.516277494921123
15	23.15122520127411	29.39573875749291	24.361315241654335	23.091720799578642
16	22.949324070126156	28.774798725891	25.773870733114297	22.50200647086855
17	21.956023119589577	28.452419618795137	27.053531455859464	22.538025805755822
18	21.88675672421923	28.29570763566319	27.841615919419727	21.975919720697853
19	22.921778213071303	27.71192291124422	28.025685383521953	21.34061349216252
20	23.24961358912218	27.213546483739393	27.804465122695248	21.73237480444318
21	23.37036196342869	27.004731621856504	27.684753342421192	21.940153072293615
22	23.106518522234204	26.866770334328212	27.53486243134107	22.49184871209651
23	22.962568635454737	26.893122127061105	27.411042744753544	22.733266492730614
24	22.62262538428692	27.036305277592632	27.62382613395601	22.71724320416444
25	22.88181716717853	27.017231887456628	27.545625438133374	22.555325507231476
26	22.59261326121702	27.133031650375493	27.715663916741022	22.558691171666464
27	22.71638821745255	27.376999622434656	27.34651843359303	22.560093726519764
28	22.719027360197135	27.159424015650522	27.501841883329625	22.619706740822718
29	22.873737560578594	27.22900072672368	27.43535513838788	22.461906574309847
30	22.95233276085745	27.2189397345292	27.333685722652053	22.4950417819613
31	22.97649704888511	26.847025465553813	27.526223946135648	22.650253539425428
32	22.806414719544126	27.159286997000308	27.372851811350664	22.6614464721049
33	22.83633618381533	27.1349458057283	27.34982925951425	22.678888750942114
34	23.11301145031706	27.052933810633668	27.4065198021323	22.427534936916974
35	23.073672145065647	27.169335011277212	27.208273843450847	22.548719000206294
36	22.968543407738952	27.110988732528646	27.315025051369734	22.605442808362668
37	23.08933433498087	26.902513692179	27.345479354815243	22.66267261802489
38	22.898952431418905	27.073361155617707	27.348532118433884	22.679154294529503
39	22.989830351110406	27.270041159130443	27.231209423502424	22.508919066256727
40	23.096059766675175	26.9518832610791	27.532028388292744	22.420028583952973
41	23.010368031880787	27.06930884388226	27.21191163018888	22.708411494048075
42	23.02503789437758	27.11273702807697	27.157407221513967	22.704817856031482
43	22.90753982465174	26.92729112088543	27.37158866476797	22.793580389694856
44	23.022366060788517	27.086390948205576	27.16015002696973	22.731092964036176
45	23.02240079076372	27.105859980632307	27.032039477975523	22.839699750628448
46	23.084864053946927	26.915073228737572	27.050918934106676	22.949143783208818
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	468.0
1	466.0
2	464.0
3	567.5
4	671.0
5	748.0
6	825.0
7	825.0
8	784.5
9	744.0
10	906.5
11	1069.0
12	1422.5
13	1776.0
14	3194.0
15	4612.0
16	6357.5
17	8103.0
18	8852.0
19	9601.0
20	9601.0
21	11074.5
22	12548.0
23	15703.0
24	18858.0
25	23850.5
26	28843.0
27	33529.5
28	38216.0
29	42952.5
30	47689.0
31	47689.0
32	53185.0
33	58681.0
34	65419.0
35	72157.0
36	77878.0
37	83599.0
38	88494.0
39	93389.0
40	99173.5
41	104958.0
42	108879.5
43	112801.0
44	112801.0
45	114747.5
46	116694.0
47	118651.5
48	120609.0
49	119874.5
50	119140.0
51	116501.0
52	113862.0
53	108457.5
54	103053.0
55	95922.5
56	88792.0
57	88792.0
58	80969.5
59	73147.0
60	64753.5
61	56360.0
62	48086.0
63	39812.0
64	32947.5
65	26083.0
66	20970.5
67	15858.0
68	12831.5
69	9805.0
70	9805.0
71	7575.0
72	5345.0
73	4158.0
74	2971.0
75	2288.0
76	1605.0
77	1218.0
78	831.0
79	610.5
80	390.0
81	390.0
82	281.0
83	172.0
84	142.0
85	112.0
86	88.5
87	65.0
88	42.5
89	20.0
90	15.5
91	11.0
92	9.0
93	7.0
94	7.0
95	7.0
96	7.0
97	6.0
98	5.0
99	8.5
100	12.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.19042662586842568
2	0.04940934513807028
3	0.06194978806651451
4	0.054362820094805746
5	0.037934839858543803
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	1.2540442928444232E-4
18	5.016177171377693E-4
19	1.881066439266635E-4
20	0.002194577512477741
21	0.002382684156404404
22	0.0
23	6.270221464222116E-5
24	1.881066439266635E-4
25	3.76213287853327E-4
26	5.643199317799905E-4
27	0.02595871686187956
28	3.1351107321110584E-4
29	6.897243610644328E-4
30	8.15128790348875E-4
31	0.001191342078202202
32	5.016177171377693E-4
33	0.0013794487221288655
34	0.002319981941762183
35	0.001943768653908856
36	0.03235434275538612
37	0.008402096762057635
38	0.012226931855233126
39	0.04909583406485917
40	0.014985829299490858
41	0.05655739760728348
42	0.05868927290511901
43	0.025896014647237342
44	0.02884301873542174
45	0.02708735672543954
46	0.024077650422612926
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
46	1594840.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	71.40697851499183
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.04282127664581	59.29836954725756
2	10.518181805671102	15.021431644286686
3	2.9778909440264605	6.379265839802587
4	1.223588730829531	3.4949109685412183
5	0.654117460908102	2.3354275738672925
6	0.39983039971376694	1.713040845720093
7	0.25578723995122443	1.2785495763324302
8	0.17921404421387993	1.0237706723812257
9	0.13400859572722426	0.8612234024326317
>10	0.5945319628534687	7.064990896899752
>50	0.016591870967645406	0.8018864640050128
>100	0.0031714533648563342	0.3136351166410159
>500	0.0	0.0
>1k	2.642151268441298E-4	0.41349745183252373
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3350	0.21005241905144087	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1631	0.10226731208146271	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	6.270221464222116E-5	0.0
10	6.270221464222116E-5	0.0	0.0	6.270221464222116E-5	0.0
11	6.270221464222116E-5	0.0	0.0	6.270221464222116E-5	0.0
12	6.270221464222116E-5	0.0	0.0	6.270221464222116E-5	6.270221464222116E-5
13	1.2540442928444232E-4	0.0	0.0	6.270221464222116E-5	6.270221464222116E-5
14	1.2540442928444232E-4	0.0	0.0	6.270221464222116E-5	6.270221464222116E-5
15	1.2540442928444232E-4	0.0	0.0	6.270221464222116E-5	1.2540442928444232E-4
16	1.2540442928444232E-4	0.0	0.0	6.270221464222116E-5	1.2540442928444232E-4
17	1.2540442928444232E-4	0.0	0.0	6.270221464222116E-5	1.2540442928444232E-4
18	1.2540442928444232E-4	0.0	0.0	6.270221464222116E-5	1.2540442928444232E-4
19	1.2540442928444232E-4	0.0	0.0	6.270221464222116E-5	1.2540442928444232E-4
20	1.2540442928444232E-4	0.0	0.0	6.270221464222116E-5	1.2540442928444232E-4
21	1.2540442928444232E-4	0.0	0.0	6.270221464222116E-5	1.2540442928444232E-4
22	1.2540442928444232E-4	0.0	0.0	1.2540442928444232E-4	1.2540442928444232E-4
23	1.2540442928444232E-4	0.0	0.0	1.2540442928444232E-4	1.2540442928444232E-4
24	1.881066439266635E-4	0.0	0.0	1.2540442928444232E-4	1.2540442928444232E-4
25	1.881066439266635E-4	0.0	0.0	1.2540442928444232E-4	1.2540442928444232E-4
26	1.881066439266635E-4	0.0	0.0	4.3891550249554816E-4	1.881066439266635E-4
27	1.881066439266635E-4	0.0	0.0	6.270221464222117E-4	1.881066439266635E-4
28	1.881066439266635E-4	0.0	0.0	0.001128639863559981	1.881066439266635E-4
29	1.881066439266635E-4	0.0	0.0	0.0025080885856888467	1.881066439266635E-4
30	1.881066439266635E-4	0.0	0.0	0.0040756439517443755	1.881066439266635E-4
31	1.881066439266635E-4	0.0	0.0	0.007148052469213213	1.881066439266635E-4
32	1.881066439266635E-4	0.0	0.0	0.010157758772039829	1.881066439266635E-4
33	1.881066439266635E-4	0.0	0.0	0.013418273933435329	1.881066439266635E-4
34	1.881066439266635E-4	0.0	0.0	0.017431215670537483	2.5080885856888465E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1080	0.0	24.691488	1
GTATCAA	2425	0.0	22.406649	1
GTATAGT	250	0.0	19.24822	1
CCGTCGT	55	0.0077935574	18.174906	9
TATCAAC	3255	0.0	16.669538	2
TCAACGC	3250	0.0	16.312744	4
ATCAACG	3240	0.0	16.302881	3
CAACGCA	3315	0.0	16.050217	5
TACCGTC	75	0.002448668	15.993917	7
AACGCAG	3430	0.0	15.795743	6
ATACCGT	105	8.5578E-5	15.232303	6
GTGTACG	120	1.5452124E-5	15.037673	1
GTATTAA	510	0.0	14.939386	1
ACAGCGT	205	4.456524E-10	14.628583	8
GTATAGC	290	0.0	14.519132	1
GTCCTAA	265	0.0	14.375636	1
TCTATAC	290	0.0	13.798676	3
TAATACG	90	0.008182407	13.337465	4
TACTCCG	120	2.650225E-4	13.334954	5
GCCCCGT	90	0.008219807	13.328265	6
>>END_MODULE