FastQCFastQC Report
Mon 6 Feb 2017
SRR2077324_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077324_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1594840
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14300.041.4811861
GTATCAA32250.037.8620451
TATCAAC45350.026.8409372
TCAACGC45050.026.785364
ATCAACG45450.026.6267593
CAACGCA46050.026.2037055
AACGCAG48600.025.0404916
ACGCAGA55650.021.6385787
CGCAGAG57850.020.8065388
AGAGTAC57300.020.10403411
TATACCG1055.82283E-419.814135
CAGAGTA60850.019.3279210
GCAGAGT63000.018.8325569
GAGTACT38550.018.60931212
GTACATG43850.018.5113181
TACATGG44850.018.1707672
AGTACTT40300.017.63004913
GTACTTT41250.017.47102414
TACGCTA2401.240187E-817.23952131
CGCTATC2208.6367436E-817.23952133
Produced by FastQC (version 0.11.5)