FastQCFastQC Report
Mon 6 Feb 2017
SRR2077323_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077323_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2246300
Sequences flagged as poor quality0
Sequence length46
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50180.22338957396607753No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22980.10230156256955884No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT22940.10212349196456395No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12550.026.364361
GTATCAA31100.023.5347461
TCAACGC40050.017.5317784
ATCAACG40300.017.525813
TAGTCGG700.001536538917.1491440
TATCAAC41200.017.1027852
CAACGCA41200.016.9912175
AACGCAG42650.016.405526
TTAGTAC3050.013.7761513
ACGCAGA51150.013.7183677
GTATAGC5750.013.2523481
TAGCCCT7700.013.2495414
GAGTACT23700.013.24347412
CGCAGAG52600.013.1881758
GTATAAT6750.013.0715161
GTACATG57650.012.6961081
ATAGCCC5400.012.5977693
ACCGTGC3700.012.4270228
AGAGTAC50550.012.41793611
GTCTTAT3400.012.3856111
Produced by FastQC (version 0.11.5)