FastQCFastQC Report
Mon 6 Feb 2017
SRR2077323_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077323_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2246300
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA40350.040.2270241
GGTATCA17850.037.8888931
TCAACGC55050.028.9662634
ATCAACG55400.028.8458353
CAACGCA55300.028.7700525
TATCAAC56250.028.7219332
AACGCAG58400.027.4209296
ACGCAGA68600.023.1878977
CGCAGAG70050.022.6988188
GTACATG57700.022.420671
GAGTACT45900.022.3189412
AGAGTAC70450.021.49380711
TACATGG58950.021.4075932
AGTACTT50650.020.70255513
CAGAGTA76500.020.37921310
ACATGGG59350.020.0338943
GTACTTT51750.019.72312714
GCAGAGT80550.019.483029
TACACCG2850.019.4593455
CATGGGG41850.017.9744744
Produced by FastQC (version 0.11.5)