FastQCFastQC Report
Mon 6 Feb 2017
SRR2077322_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077322_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1911615
Sequences flagged as poor quality0
Sequence length46
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36460.19072878168459653No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10050.026.3246751
GTATCAA25950.023.7113421
TATCAAC36200.016.9212742
ATCAACG36650.016.4309463
TCAACGC36800.016.415764
CAACGCA36900.016.2603025
AACGCAG38350.015.6397756
GTCCTAC2054.292815E-1014.6653531
ACGCAGA44600.013.2239687
ACTATAC2802.1827873E-1112.861293
GCGATAC1253.7571808E-412.79575714
CGCAGAG45050.012.781228
TTAGGCA3005.456968E-1212.6687634
CTAATAC2601.1605152E-912.3116643
GTATTAG2955.0931703E-1112.2294131
CGATACT1150.002922258312.17019615
AGAGTAC43800.012.09612811
TATACTG5150.012.0389385
GCACCGT2551.1563316E-811.7604986
TGCGATA1200.004019474611.66249313
Produced by FastQC (version 0.11.5)