FastQCFastQC Report
Mon 6 Feb 2017
SRR2077322_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077322_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1911615
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA31250.036.4879841
GGTATCA14750.035.950371
TCAACGC43700.025.5294484
ATCAACG44650.025.2183953
TATCAAC45000.025.0229112
CGCGATC700.001334529624.64171623
CAACGCA44950.024.588275
AACGCAG46200.024.072996
GTACATG46700.020.0378381
ACGCAGA55850.019.6474067
TATACCG1258.463714E-519.402385
TACATGG48450.019.0934522
CGCAGAG57650.018.9666568
CGAATCG1501.7024393E-518.39048637
GAGTACT38550.017.89613212
ACATGGG49900.017.7744033
AGAGTAC59400.017.47968511
GTATTAC3900.016.8846841
CAGAGTA63050.016.68574310
GCAGAGT65000.016.5035699
Produced by FastQC (version 0.11.5)