FastQCFastQC Report
Mon 6 Feb 2017
SRR2077321_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077321_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1901336
Sequences flagged as poor quality0
Sequence length46
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39380.20711752157430355No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19150.10071865256850972No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10800.024.8738881
GTATCAA28200.023.8154241
TATCAAC37200.017.6521842
ATCAACG38350.017.1673663
TCAACGC38350.017.1642044
CAACGCA38800.016.9095635
AACGCAG40050.016.4734546
GTGTACG1655.700713E-915.7950871
CTATGCG904.594444E-415.549949
CGTGCCG1058.557779E-515.232594510
CGAATCG956.8901473E-414.74160337
ACGCAGA45350.014.2837087
CGCAGAG46450.013.7732848
GTACCGA1752.0666994E-713.7093366
GAGTACT21300.013.51660212
GTATTAG2555.820766E-1113.3650731
TAGAACC3450.013.3383424
AGAGTAC45200.013.09296111
CAGAGTA48250.012.63792410
GCAGAGT49450.012.6142529
Produced by FastQC (version 0.11.5)