FastQCFastQC Report
Mon 6 Feb 2017
SRR2077321_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077321_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1901336
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17400.039.6416171
GTATCAA40800.039.334021
TATCAAC58800.027.1600192
TCAACGC58750.026.7682514
ATCAACG58950.026.6774353
CAACGCA59400.026.357315
AACGCAG61650.025.618756
TAGCCCG1151.6407466E-624.0957955
TAGACCG1301.778917E-723.9799485
ACGCAGA71200.021.9069217
CGCAGAG73350.021.4411328
AGAGTAC72900.020.34395211
GAGTACT52100.020.0585412
CAGAGTA77300.019.58700410
GTACATG46700.019.4460721
GCAGAGT79700.019.2568249
AGTACTT54350.019.1646913
GTACCGT1651.7620987E-618.8922946
TACATGG48350.018.198842
TATACCG1150.001081291218.0718465
Produced by FastQC (version 0.11.5)