FastQCFastQC Report
Mon 6 Feb 2017
SRR2077320_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077320_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1622416
Sequences flagged as poor quality0
Sequence length46
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28480.1755406751412708No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA23950.023.5298961
GGTATCA9450.022.7075961
ACCGTCG500.004492508219.993658
TATCAAC30800.017.7446142
ATCAACG31400.017.4596023
TCAACGC31000.017.4240284
TAGACCG700.001538570417.1453425
CAACGCA31900.016.8049435
ATACCGT852.972043E-416.465366
AACGCAG33350.016.3066656
TGTACCG1557.8381527E-714.1956055
ACGCAGA38000.014.1007867
GTATAAT4650.013.801181
CGCAGAG38550.013.7958788
GAGTACT16300.013.49264812
GTATAAC2251.713488E-913.3698931
ATAACAC3000.013.3389663
TTAGTAC1551.2170798E-512.9086783
AGAGTAC37550.012.88538911
GTATTAG2059.840551E-812.7177041
Produced by FastQC (version 0.11.5)