##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2077320_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1622416 Sequences flagged as poor quality 0 Sequence length 46 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.440791387658898 31.0 31.0 34.0 27.0 34.0 2 30.59150674056469 31.0 31.0 34.0 27.0 34.0 3 30.751544610013706 33.0 31.0 34.0 27.0 34.0 4 34.16467663040798 37.0 35.0 37.0 30.0 37.0 5 34.24680229978008 37.0 35.0 37.0 30.0 37.0 6 34.288261456987605 37.0 35.0 37.0 30.0 37.0 7 34.182956159209475 37.0 35.0 37.0 30.0 37.0 8 34.175793384680624 37.0 35.0 37.0 30.0 37.0 9 35.716752053727284 39.0 37.0 39.0 30.0 39.0 10 35.64925641758957 39.0 37.0 39.0 30.0 39.0 11 35.69131036676167 39.0 37.0 39.0 30.0 39.0 12 35.62616677843414 39.0 37.0 39.0 30.0 39.0 13 35.594324760110844 39.0 37.0 39.0 30.0 39.0 14 36.70450796836323 40.0 37.0 41.0 29.0 41.0 15 36.83181132335973 40.0 38.0 41.0 30.0 41.0 16 36.81917153183894 40.0 38.0 41.0 30.0 41.0 17 36.834848768749815 40.0 38.0 41.0 30.0 41.0 18 36.79431354227276 40.0 38.0 41.0 30.0 41.0 19 36.87527551503437 40.0 38.0 41.0 30.0 41.0 20 36.86135245214544 40.0 38.0 41.0 30.0 41.0 21 36.87511156201615 40.0 38.0 41.0 30.0 41.0 22 36.8318557016203 40.0 38.0 41.0 30.0 41.0 23 36.788270702458554 40.0 38.0 41.0 30.0 41.0 24 36.81450749992604 40.0 38.0 41.0 30.0 41.0 25 36.75761272078184 40.0 38.0 41.0 30.0 41.0 26 36.786038229406024 40.0 38.0 41.0 30.0 41.0 27 36.756961839626825 40.0 38.0 41.0 30.0 41.0 28 36.69877392727882 40.0 37.0 41.0 30.0 41.0 29 36.66136983363083 40.0 37.0 41.0 30.0 41.0 30 36.58039676630408 40.0 37.0 41.0 30.0 41.0 31 36.550709559077326 40.0 37.0 41.0 30.0 41.0 32 36.521481543574524 40.0 37.0 41.0 30.0 41.0 33 36.47641295450735 40.0 37.0 41.0 30.0 41.0 34 36.42785327560872 40.0 37.0 41.0 30.0 41.0 35 36.4096304523624 40.0 37.0 41.0 30.0 41.0 36 36.35081446435439 40.0 37.0 41.0 29.0 41.0 37 36.265902210037375 40.0 37.0 41.0 29.0 41.0 38 36.23555117799627 40.0 37.0 41.0 29.0 41.0 39 36.15407700614392 40.0 37.0 41.0 28.0 41.0 40 36.13341954221359 40.0 37.0 41.0 28.0 41.0 41 36.002392111517636 40.0 36.0 41.0 28.0 41.0 42 35.95714600940819 40.0 36.0 41.0 28.0 41.0 43 35.86164152720387 40.0 36.0 41.0 27.0 41.0 44 35.77606421534304 40.0 36.0 41.0 27.0 41.0 45 35.67947431484897 40.0 36.0 41.0 27.0 41.0 46 35.579530773858245 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2582741275909868 1101 2 0.345178926428769 1101 3 0.5160645285311602 1101 4 0.5948382522540072 1101 5 0.4685889827381331 1101 6 0.3751266685239685 1101 7 0.5369399602905744 1101 8 0.6173065956553287 1101 9 0.5776793954900654 1101 10 0.6785672203434245 1101 11 0.7183320297180416 1101 12 0.6482963887113726 1101 13 0.7272996718551212 1101 14 0.9542189227394431 1101 15 0.9156990723141476 1101 16 0.656317899874189 1101 17 0.6247294775974126 1101 18 0.649027684585441 1101 19 0.6953060941262876 1101 20 0.5512230851068196 1101 21 0.5480338725764966 1101 22 0.6426346804611072 1101 23 0.7802357485407398 1101 24 0.5992628290798905 1101 25 0.634129905875092 1101 26 0.7607387297781614 1101 27 0.6566466694644149 1101 28 0.6154340629422208 1101 29 0.6131999233044212 1101 30 0.5254943929166558 1101 31 0.6352172039563371 1101 32 0.517011819451767 1101 33 0.523892447192722 1101 34 0.4377328612071949 1101 35 0.6082074701590017 1101 36 0.49208469317879633 1101 37 0.5451974842184697 1101 38 0.4223432419196982 1101 39 0.5116025170920437 1101 40 0.4517797395878347 1101 41 0.48448067913189874 1101 42 0.4448960125477228 1101 43 0.4238830295860936 1101 44 0.3694593364531187 1101 45 0.43523126511574617 1101 46 0.45836358877686934 1102 1 0.3857605344781625 1102 2 0.46312378838836565 1102 3 0.5477622614499325 1102 4 0.5486453003611302 1102 5 0.5861084740119793 1102 6 0.5496593076190095 1102 7 0.48432152834207187 1102 8 0.5691922494528043 1102 9 0.5601081488297552 1102 10 0.6370049107641833 1102 11 0.6945475977382927 1102 12 0.7117141191302636 1102 13 0.7862449556699431 1102 14 1.0127267270611142 1102 15 0.816996472436017 1102 16 0.9163361802672938 1102 17 0.8095360491643717 1102 18 0.8230025373267225 1102 19 0.8051767251904565 1102 20 0.8171158948188619 1102 21 0.7512956337439434 1102 22 0.806820538936492 1102 23 0.7712187883982438 1102 24 0.8128914871115427 1102 25 0.875652373571917 1102 26 0.8446900679966802 1102 27 0.889648442818789 1102 28 0.8569614132226775 1102 29 0.9862341951354239 1102 30 0.9326887822421597 1102 31 0.8567074075405401 1102 32 0.873968329302869 1102 33 0.917197253217438 1102 34 0.8741743170764522 1102 35 0.8583322689965556 1102 36 0.8263034016768316 1102 37 0.9835693183512433 1102 38 0.8390069139350729 1102 39 0.9383903239136018 1102 40 0.8416800176997938 1102 41 0.9276665454194131 1102 42 0.8175412401151831 1102 43 0.8106338459540794 1102 44 0.9328895662861001 1102 45 0.9162311791822759 1102 46 0.807461482625591 1103 1 0.34543034579891696 1103 2 0.3585709404555537 1103 3 0.41490309069225617 1103 4 0.4638217065834027 1103 5 0.43505269829777404 1103 6 0.40378703577894015 1103 7 0.4165322990287734 1103 8 0.39635925128636273 1103 9 0.6297989573705962 1103 10 0.5706721787596862 1103 11 0.603710962025275 1103 12 0.5765289887109049 1103 13 0.6319700991016575 1103 14 0.8133669944060031 1103 15 0.7144864174292138 1103 16 0.7537969953143175 1103 17 0.6689389592016326 1103 18 0.5920205476183114 1103 19 0.6506578868394755 1103 20 0.6561803355830449 1103 21 0.6218810421546621 1103 22 0.7574925978469977 1103 23 0.6721629239633984 1103 24 0.8197895175131507 1103 25 0.6904206648122297 1103 26 0.5896633039344579 1103 27 0.6555405289369602 1103 28 0.7060165383187922 1103 29 0.7631867887357089 1103 30 0.7129151677629508 1103 31 0.8172067569224524 1103 32 0.8696749892661941 1103 33 0.7743607062250675 1103 34 0.8026026307926628 1103 35 0.6973109464674252 1103 36 0.7866452719080996 1103 37 0.781876971985831 1103 38 0.7742097884840788 1103 39 0.6685435148995822 1103 40 0.5981810232004747 1103 41 0.711866876643164 1103 42 0.6882479000785082 1103 43 0.6680797263586413 1103 44 0.79405505986864 1103 45 0.8312109213009222 1103 46 0.6505848930473093 1104 1 0.33894845394125284 1104 2 0.4092820850789458 1104 3 0.43265580461123676 1104 4 0.394291562800376 1104 5 0.4339561944470631 1104 6 0.4643983726502512 1104 7 0.4943394640051153 1104 8 0.4017223350811321 1104 9 0.4222870426043457 1104 10 0.5738048413358143 1104 11 0.6115922633249653 1104 12 0.5650380024905957 1104 13 0.671549283371867 1104 14 0.8636061531198962 1104 15 0.8371509505660768 1104 16 0.7224516627944837 1104 17 0.6644076066742102 1104 18 0.590703879608597 1104 19 0.6585873940171183 1104 20 0.6576042077093973 1104 21 0.6382199568173093 1104 22 0.6403602288230914 1104 23 0.5970056432929454 1104 24 0.6336939633901792 1104 25 0.5902265462171385 1104 26 0.6952831020165888 1104 27 0.6646715856098098 1104 28 0.6588273007487189 1104 29 0.7662475022402617 1104 30 0.770049925496032 1104 31 0.7575955121459614 1104 32 0.7641818215500322 1104 33 0.9267725830022115 1104 34 0.7379211349426811 1104 35 0.6655152127595727 1104 36 0.7586685949191363 1104 37 0.5796900080064162 1104 38 0.6620339910005697 1104 39 0.6771313560820786 1104 40 0.6822232947190088 1104 41 0.7867385194994867 1104 42 0.7996171484697285 1104 43 0.6835394233667103 1104 44 0.6301500383990373 1104 45 0.7342854490272188 1104 46 0.6087227647362141 1105 1 0.19699062487850938 1105 2 0.16299278953135854 1105 3 0.44059183676128555 1105 4 0.3716318887501018 1105 5 0.39817669732606475 1105 6 0.4848536535153656 1105 7 0.3125517820271142 1105 8 0.4515591724269612 1105 9 0.5920193590662208 1105 10 0.49368937793654055 1105 11 0.4004418041004598 1105 12 0.49817002484077477 1105 13 0.6243025357907754 1105 14 0.7905025668639851 1105 15 0.5668831009039579 1105 16 0.6222077494332083 1105 17 0.4113577868103988 1105 18 0.34779267938440483 1105 19 0.39317307355092623 1105 20 0.5631015034996665 1105 21 0.3854115584548552 1105 22 0.3091846415386499 1105 23 0.5337028310303964 1105 24 0.4534517762738943 1105 25 0.4210720523463891 1105 26 0.4990462438691452 1105 27 0.39675782129786796 1105 28 0.5940467072093512 1105 29 0.45931355891887904 1105 30 0.6323753869596018 1105 31 0.5862472786940742 1105 32 0.5738163632067099 1105 33 0.6024001344500931 1105 34 0.5039735882329524 1105 35 0.6104306628689216 1105 36 0.6585803325183477 1105 37 0.7287967665083528 1105 38 0.6258012642846538 1105 39 0.5835385418838186 1105 40 0.3871126850294999 1105 41 0.4223668437850847 1105 42 0.6494921582204825 1105 43 0.5957784642389896 1105 44 0.5518346729406929 1105 45 0.5047732324130862 1105 46 0.4853740733599281 1106 1 0.12100686571506003 1106 2 0.09522998371837232 1106 3 0.27953049682909636 1106 4 0.2422665321980375 1106 5 0.26385010634046324 1106 6 0.16848114669947023 1106 7 0.2860863299035614 1106 8 0.2538873625880598 1106 9 0.1987431159373969 1106 10 0.3648974503434488 1106 11 0.3678523761666881 1106 12 0.22155471769890056 1106 13 0.2580135525036056 1106 14 0.44952098716376554 1106 15 0.3685172922148041 1106 16 0.4006237534618293 1106 17 0.44742190183551855 1106 18 0.3699889104923386 1106 19 0.2499717804929915 1106 20 0.2770875206411674 1106 21 0.25518578772356193 1106 22 0.31729820104882833 1106 23 0.38383626110843494 1106 24 0.3148343050107698 1106 25 0.2860848538726444 1106 26 0.2757887089277915 1106 27 0.4053690023503407 1106 28 0.46369711769748534 1106 29 0.3014848638262393 1106 30 0.45961804559789954 1106 31 0.4652151426666933 1106 32 0.3929835465370175 1106 33 0.555940656672945 1106 34 0.5860598444385516 1106 35 0.40509634764904945 1106 36 0.3435158646277472 1106 37 0.3097214545472937 1106 38 0.28848461219286037 1106 39 0.33841201118317343 1106 40 0.26720141580577206 1106 41 0.32644663650594197 1106 42 0.36407218061977176 1106 43 0.4078262481806334 1106 44 0.45303161790180013 1106 45 0.4561203933802034 1106 46 0.39060776325886337 1107 1 0.38261887806159933 1107 2 0.3199895898050009 1107 3 0.3304777610187202 1107 4 0.3492389226766619 1107 5 0.2770615214734846 1107 6 0.32707073259532393 1107 7 0.287449400138037 1107 8 0.3671784577631598 1107 9 0.5381646233600179 1107 10 0.44816850946913434 1107 11 0.4662131333591688 1107 12 0.42341443440927407 1107 13 0.3572608468633405 1107 14 0.6567092099893941 1107 15 0.5308696327193374 1107 16 0.6229125046512252 1107 17 0.5343953753438413 1107 18 0.6668473507748516 1107 19 0.5777538130302986 1107 20 0.555809855034326 1107 21 0.5367410183702077 1107 22 0.49371755014797714 1107 23 0.45371179005240236 1107 24 0.5884230786343565 1107 25 0.457882760530957 1107 26 0.4963585372675823 1107 27 0.5547837795169812 1107 28 0.6591545817060904 1107 29 0.6410982222239738 1107 30 0.624948717772547 1107 31 0.6686266862160863 1107 32 0.6313676662272698 1107 33 0.5647890093074182 1107 34 0.551613311293977 1107 35 0.553811797067695 1107 36 0.699767653560734 1107 37 0.5677486385927253 1107 38 0.5487335543049241 1107 39 0.5235806930586691 1107 40 0.48726840892853573 1107 41 0.6101137750641215 1107 42 0.5049836274700894 1107 43 0.3880594090028069 1107 44 0.4589697150186183 1107 45 0.3946785714148149 1107 46 0.33269352373910976 1108 1 -0.08806632636326128 1108 2 -0.12237448189397071 1108 3 -0.16440713928659534 1108 4 -0.2100827991813361 1108 5 -0.1934166905901904 1108 6 -0.2136385944008552 1108 7 -0.18412646853327175 1108 8 -0.08314984406354853 1108 9 -0.12196834932893097 1108 10 -0.01741005408349139 1108 11 -0.21248844734596162 1108 12 -0.24372891178146716 1108 13 -0.21351511575065274 1108 14 -0.046262225064765516 1108 15 -0.053962404921421125 1108 16 0.007143567835647957 1108 17 -0.023682899613667985 1108 18 0.11794590302250896 1108 19 -0.03932723642097358 1108 20 0.014059595298718364 1108 21 0.017584273404693818 1108 22 0.024964440286787237 1108 23 0.026621366235538346 1108 24 0.17403979645201417 1108 25 -0.007369889568359156 1108 26 -0.1188282335321631 1108 27 -0.10734547527071214 1108 28 -0.09842303047779666 1108 29 0.11277559676470617 1108 30 -0.19857586456065235 1108 31 0.024669967522690683 1108 32 -0.03439987737049677 1108 33 -0.009798896765822462 1108 34 0.009908331137381765 1108 35 -0.12118034120319976 1108 36 -0.09418538305700963 1108 37 -0.007284102025948869 1108 38 -0.12095291197721281 1108 39 0.015555260862598175 1108 40 0.03141749119605919 1108 41 0.06058139867239731 1108 42 -0.014370655878572336 1108 43 0.017810779332045 1108 44 -0.16581035559762114 1108 45 -0.13516514935248836 1108 46 -0.14976287849481906 1109 1 0.19137288064727898 1109 2 0.12269005031496505 1109 3 0.21331440994892503 1109 4 0.21594331818429424 1109 5 0.21604920035385078 1109 6 0.15675691750799103 1109 7 0.1210552828423559 1109 8 0.14838161715481846 1109 9 0.09257057234209043 1109 10 0.18874838450170728 1109 11 0.08433931859084964 1109 12 0.1791584388520988 1109 13 0.15421425109369125 1109 14 0.30145001627002443 1109 15 0.11094128496424815 1109 16 0.11207408939927177 1109 17 0.2733904111854244 1109 18 0.23314048889480432 1109 19 0.2945492183801477 1109 20 0.18207410894324028 1109 21 0.20256033237914295 1109 22 0.18188607534256107 1109 23 0.011768712423929628 1109 24 0.23995100717286988 1109 25 0.11105965752422264 1109 26 0.23331120254651694 1109 27 0.15496565271324414 1109 28 0.06055787323007422 1109 29 0.08901427343688795 1109 30 0.150635661819436 1109 31 0.2799577737230692 1109 32 0.18714148915009332 1109 33 0.07897207491735259 1109 34 0.30975955889380913 1109 35 0.30848301984185156 1109 36 0.19979589322383617 1109 37 0.23307428748239545 1109 38 0.395038658880388 1109 39 0.3130340659568276 1109 40 0.30799960949183003 1109 41 0.2839679649756164 1109 42 0.3861232923800344 1109 43 0.3207964378419348 1109 44 0.25908872851522347 1109 45 0.31102471383614727 1109 46 0.28574634525580933 1110 1 -0.004754838009024098 1110 2 0.01740898812196079 1110 3 0.03841440286050357 1110 4 0.057400040551144116 1110 5 0.13929238658860044 1110 6 0.12751412528309913 1110 7 0.09086689824679439 1110 8 0.08965271049149948 1110 9 0.11472040468448341 1110 10 0.1660982942064777 1110 11 0.03535910467697789 1110 12 0.14337145266294016 1110 13 0.17361634191217945 1110 14 0.1825862543333514 1110 15 0.2310157118308993 1110 16 0.16801206680175795 1110 17 0.16296155852242578 1110 18 0.16145839069684342 1110 19 0.3097973061100845 1110 20 0.2277887979219031 1110 21 0.34926312861298925 1110 22 0.21034475977837275 1110 23 0.15215999645011635 1110 24 0.18612453456101719 1110 25 0.22490362742863113 1110 26 0.15188479296372037 1110 27 0.19647720472872976 1110 28 0.16139748352020433 1110 29 0.2655732529419552 1110 30 0.15075740116486003 1110 31 0.1797245130004228 1110 32 0.08183789422478327 1110 33 0.07796529991935586 1110 34 0.10688281747741257 1110 35 0.09509898368808223 1110 36 0.07624563592037248 1110 37 0.16586462369423316 1110 38 0.1429027045104192 1110 39 0.1957034686813799 1110 40 0.21136845639333757 1110 41 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-0.8258259019179874 2215 36 -1.0209409761342627 2215 37 -1.0476578180805376 2215 38 -0.811843161027447 2215 39 -0.8846801408540159 2215 40 -0.6795666089868604 2215 41 -0.6494024200978288 2215 42 -0.7911311736779254 2215 43 -0.8586442649904527 2215 44 -0.865804586637438 2215 45 -0.9672853125718532 2215 46 -1.029294889188499 2216 1 -1.4464278507909576 2216 2 -1.4792714445900472 2216 3 -1.2697868251965474 2216 4 -1.1329984917654699 2216 5 -1.043851475191893 2216 6 -1.171060874074847 2216 7 -1.2839321104192791 2216 8 -1.3341204886540652 2216 9 -1.6311820240724657 2216 10 -1.6494303241679233 2216 11 -1.570644841452875 2216 12 -1.712709540144914 2216 13 -1.8735626051388863 2216 14 -3.760322017688324 2216 15 -2.557382007587428 2216 16 -2.0577211607505816 2216 17 -2.0146995667387273 2216 18 -2.324766584486312 2216 19 -2.177894487078767 2216 20 -1.924623628478983 2216 21 -1.9761770144928406 2216 22 -2.041046267745159 2216 23 -2.158642254876149 2216 24 -1.946095520800931 2216 25 -2.1457125282547906 2216 26 -2.0955866243936683 2216 27 -2.2273719244763157 2216 28 -2.2160360469707356 2216 29 -2.3388915741329157 2216 30 -2.149289580559447 2216 31 -2.229288245482806 2216 32 -2.121253659561056 2216 33 -1.9740855933196286 2216 34 -1.924698643751917 2216 35 -2.00819478971205 2216 36 -2.2322220452314383 2216 37 -2.0375196237216286 2216 38 -2.1983477798228606 2216 39 -2.1433645847510476 2216 40 -2.2844763269324204 2216 41 -2.3557197005510844 2216 42 -2.11075440313968 2216 43 -2.0450037920258026 2216 44 -2.1198749154956715 2216 45 -2.2449671699434504 2216 46 -2.2736719060625035 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 45834.0 3 1441.0 4 4495.0 5 3337.0 6 2689.0 7 2302.0 8 1943.0 9 1806.0 10 1754.0 11 1661.0 12 1684.0 13 1710.0 14 1802.0 15 1941.0 16 2014.0 17 2343.0 18 2503.0 19 3093.0 20 4112.0 21 5744.0 22 7576.0 23 8985.0 24 9564.0 25 10055.0 26 11077.0 27 12635.0 28 14463.0 29 16228.0 30 18055.0 31 21967.0 32 27724.0 33 34784.0 34 45118.0 35 61541.0 36 85335.0 37 125980.0 38 233206.0 39 783915.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.90322530840371 18.128240361901526 13.61237629112693 24.356158038567834 2 15.381209336745707 20.14714316548981 39.006629459467796 25.465018038296694 3 18.164397029354276 25.3143250822935 29.51291253914222 27.00836534921 4 12.709671371462614 16.722706489066066 38.07270704411933 32.494915095351985 5 14.030046591702286 37.52124251116696 34.59514055204631 13.85357034508444 6 31.830184120472182 37.0638603169594 17.75555714440686 13.350398418161557 7 28.1504250451179 32.00584806855949 22.07343862486563 17.770288261456987 8 26.602733207759293 34.164357353477776 20.358280490330472 18.874628948432463 9 26.76927495784065 15.843901933905977 19.274526385341368 38.112296722912006 10 14.709790830465183 28.7142138637686 33.75089989250599 22.825095413260225 11 34.093598682458754 23.351963984576088 24.15989487283163 18.39454246013353 12 23.509568446070553 24.789573081133323 30.247051311131052 21.453807161665072 13 27.648889064210415 22.147772211319413 26.180215185254585 24.02312353921559 14 22.399618840050888 21.232778769440145 27.0973042672163 29.27029812329267 15 23.513342763719518 29.02808467623882 23.71205887519531 23.746513684846356 16 23.60202315559018 28.225683178666877 25.165062474729044 23.007231191013897 17 22.423280892892805 27.989253038069243 26.584862537998976 23.00260353103897 18 22.61108179939941 27.767244737451986 27.148411496984732 22.473261966163875 19 23.477096740591627 27.331976915836986 27.12728155812553 22.063644785445856 20 23.77449318597871 26.79016759225587 27.048736740241246 22.38660248152417 21 23.78512083917101 26.604465215244677 27.044995478817007 22.5654184667673 22 23.727268468752776 26.305522134890186 26.885706255362372 23.081503140994663 23 23.572049780173113 26.417132998811034 26.814010982407073 23.19680623860879 24 23.20566318008395 26.66761176274801 26.856281704378056 23.270443352789986 25 23.404423181113003 26.49550944984828 26.9232689454619 23.176798423576823 26 23.229185350372255 26.684985173227616 27.00056644372576 23.085263032674373 27 23.315886168035313 26.869867574165866 26.707602771818205 23.106643485980616 28 23.274800959315385 26.655087163701836 26.840182518936373 23.22992935804641 29 23.36501479289941 26.773853550295858 26.748952169625245 23.11217948717949 30 23.52183038039442 26.70225191768959 26.75772546143202 23.018192240483977 31 23.598797331347786 26.404346175277 26.796359942603264 23.200496550771945 32 23.388099866740795 26.73930382235722 26.67729699544256 23.195299315459426 33 23.464500147005616 26.619850874051632 26.6732906513674 23.242358327575356 34 23.60363147964489 26.513388754346266 26.771344866673363 23.111634899335478 35 23.560089375144464 26.633238308036056 26.70831342938593 23.098358887433545 36 23.534862786926684 26.65299982120067 26.67013989506329 23.141997496809356 37 23.66582834036048 26.344157605345565 26.74612274083389 23.243891313460068 38 23.49309284133569 26.53150293115033 26.76457715613692 23.210827071377054 39 23.656321109007603 26.739823510911858 26.46078328595302 23.143072094127515 40 23.571497702769932 26.479760765048816 26.87768428852445 23.071057243656803 41 23.45801135978193 26.633446602241122 26.74969627075997 23.158845767216977 42 23.560758977660768 26.60300944127556 26.58327459131901 23.25295698974466 43 23.49987361516125 26.489090830625823 26.668988859638848 23.342046694574083 44 23.4900992235483 26.59775560205525 26.561010706574745 23.351134467821705 45 23.5586858628156 26.646370635613494 26.407466867900702 23.387476633670204 46 23.657416797725826 26.447235304163087 26.499453466342715 23.395894431768372 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 372.0 1 394.0 2 416.0 3 507.0 4 598.0 5 642.5 6 687.0 7 687.0 8 652.5 9 618.0 10 745.0 11 872.0 12 1171.5 13 1471.0 14 2561.0 15 3651.0 16 5163.5 17 6676.0 18 7148.0 19 7620.0 20 7620.0 21 8896.0 22 10172.0 23 12809.0 24 15446.0 25 20166.5 26 24887.0 27 29021.0 28 33155.0 29 38265.5 30 43376.0 31 43376.0 32 48401.0 33 53426.0 34 60097.5 35 66769.0 36 72495.5 37 78222.0 38 83812.0 39 89402.0 40 95038.5 41 100675.0 42 105008.0 43 109341.0 44 109341.0 45 113189.0 46 117037.0 47 120040.0 48 123043.0 49 124416.5 50 125790.0 51 123624.5 52 121459.0 53 117402.5 54 113346.0 55 106642.0 56 99938.0 57 99938.0 58 91549.5 59 83161.0 60 74200.0 61 65239.0 62 56136.0 63 47033.0 64 39451.5 65 31870.0 66 25852.0 67 19834.0 68 15946.0 69 12058.0 70 12058.0 71 9347.0 72 6636.0 73 5211.5 74 3787.0 75 2960.0 76 2133.0 77 1584.0 78 1035.0 79 800.5 80 566.0 81 566.0 82 423.5 83 281.0 84 217.0 85 153.0 86 112.0 87 71.0 88 47.0 89 23.0 90 21.5 91 20.0 92 15.5 93 11.0 94 11.0 95 13.0 96 15.0 97 13.5 98 12.0 99 12.5 100 13.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.19668198538475953 2 0.053377185629333045 3 0.06687557321919903 4 0.05855464936243232 5 0.041173163972741886 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 6.163647301308666E-5 16 0.0 17 2.4654589205234663E-4 18 4.930917841046933E-4 19 3.081823650654333E-4 20 0.001602548298340253 21 0.003266733069693593 22 0.0 23 1.8490941903925997E-4 24 3.6981883807851994E-4 25 5.547282571177799E-4 26 8.012741491701265E-4 27 0.023175313852920584 28 5.547282571177799E-4 29 9.861835682093865E-4 30 0.0012943659332748199 31 0.0014792753523140798 32 8.629106221832132E-4 33 0.001787457717379513 34 0.002896914231615073 35 0.0025270953935365528 36 0.029955325884360114 37 0.008382560329779784 38 0.011464383980434117 39 0.04844626778828611 40 0.01510093588820623 41 0.05584264454985651 42 0.05664391869902663 43 0.02379167858305145 44 0.02601059161152257 45 0.026133864557548743 46 0.023113677379907493 >>END_MODULE >>Sequence Length Distribution pass #Length Count 46 1622416.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.03832634942047 #Duplication Level Percentage of deduplicated Percentage of total 1 83.44166310007675 59.27556094441648 2 10.134127198730623 14.398228704199287 3 2.8923088818042957 6.163943467468228 4 1.2363751589070089 3.5132008811501074 5 0.6473481980298831 2.299326627667805 6 0.38926438581010897 1.6591614285231142 7 0.2614223116936537 1.2999702445179582 8 0.2001107289312152 1.1372425014268865 9 0.13319556856884182 0.8515791241460999 >10 0.6400388798391609 7.7270741398622445 >50 0.019703738849194716 0.9199419213192591 >100 0.004180636856651073 0.42280216669960463 >500 0.0 0.0 >1k 2.612119025264346E-4 0.33196784860288286 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2848 0.1755406751412708 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 6.163647301308666E-5 0.0 6 0.0 0.0 0.0 6.163647301308666E-5 0.0 7 0.0 0.0 0.0 6.163647301308666E-5 0.0 8 0.0 0.0 0.0 6.163647301308666E-5 0.0 9 0.0 0.0 0.0 6.163647301308666E-5 0.0 10 0.0 0.0 0.0 6.163647301308666E-5 0.0 11 0.0 0.0 0.0 6.163647301308666E-5 0.0 12 0.0 0.0 0.0 6.163647301308666E-5 0.0 13 0.0 0.0 0.0 6.163647301308666E-5 0.0 14 0.0 0.0 0.0 6.163647301308666E-5 0.0 15 0.0 0.0 0.0 6.163647301308666E-5 0.0 16 0.0 0.0 0.0 1.2327294602617331E-4 0.0 17 0.0 0.0 0.0 1.2327294602617331E-4 0.0 18 0.0 0.0 0.0 1.2327294602617331E-4 0.0 19 0.0 0.0 0.0 1.2327294602617331E-4 0.0 20 0.0 0.0 0.0 1.2327294602617331E-4 0.0 21 0.0 0.0 0.0 1.8490941903925997E-4 0.0 22 0.0 0.0 0.0 2.4654589205234663E-4 0.0 23 0.0 0.0 0.0 2.4654589205234663E-4 0.0 24 0.0 0.0 0.0 3.6981883807851994E-4 0.0 25 0.0 0.0 0.0 3.6981883807851994E-4 0.0 26 0.0 0.0 0.0 4.930917841046933E-4 0.0 27 0.0 0.0 0.0 6.163647301308666E-4 0.0 28 0.0 0.0 0.0 8.629106221832132E-4 0.0 29 0.0 0.0 0.0 0.0014792753523140798 0.0 30 0.0 0.0 0.0 0.0025270953935365528 0.0 31 0.0 0.0 0.0 0.005177463733099279 0.0 32 0.0 0.0 0.0 0.007827832072662005 0.0 33 0.0 0.0 0.0 0.011402747507421032 0.0 34 0.0 0.0 0.0 0.016148755929428705 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 2395 0.0 23.529896 1 GGTATCA 945 0.0 22.707596 1 ACCGTCG 50 0.0044925082 19.99365 8 TATCAAC 3080 0.0 17.744614 2 ATCAACG 3140 0.0 17.459602 3 TCAACGC 3100 0.0 17.424028 4 TAGACCG 70 0.0015385704 17.145342 5 CAACGCA 3190 0.0 16.804943 5 ATACCGT 85 2.972043E-4 16.46536 6 AACGCAG 3335 0.0 16.306665 6 TGTACCG 155 7.8381527E-7 14.195605 5 ACGCAGA 3800 0.0 14.100786 7 GTATAAT 465 0.0 13.80118 1 CGCAGAG 3855 0.0 13.795878 8 GAGTACT 1630 0.0 13.492648 12 GTATAAC 225 1.713488E-9 13.369893 1 ATAACAC 300 0.0 13.338966 3 TTAGTAC 155 1.2170798E-5 12.908678 3 AGAGTAC 3755 0.0 12.885389 11 GTATTAG 205 9.840551E-8 12.717704 1 >>END_MODULE