FastQCFastQC Report
Mon 6 Feb 2017
SRR2077320_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077320_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1622416
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13750.041.8637051
GTATCAA29400.036.681351
ACCGTCG450.004631732630.6551728
ATCAACG41400.025.3664233
TATCAAC41650.025.3012982
TCAACGC42050.024.9735414
CAACGCA42900.024.3156435
AACGCAG44900.023.3854646
ACGCAGA52250.019.8769957
GTACATG42000.019.319631
CGCAGAG54000.019.1594838
TACATGG44350.017.898892
AGAGTAC54000.017.88328611
ACATGGG45000.017.0984753
GAGTACT34300.016.9932212
CAGAGTA58500.016.68400210
GCAGAGT61250.016.3848789
TACGACA1704.772527E-516.3096474
GTGGTAT6900.016.0817411
AGTACTT36750.016.04803513
Produced by FastQC (version 0.11.5)