FastQCFastQC Report
Mon 6 Feb 2017
SRR2077319_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077319_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2080637
Sequences flagged as poor quality0
Sequence length46
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45120.2168566645695525No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT21460.10314148984181287No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21030.10107481506865446No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11550.024.6505781
GTATCAA28100.023.7606681
TATACCG902.2772358E-517.779215
TATCAAC37550.017.7015362
ATCAACG38200.017.1279743
TCAACGC38100.016.9083144
CGCACGA1208.572588E-716.67001335
CAACGCA38700.016.6421665
AACGCAG40150.015.985566
GCGATAC1301.9854306E-615.38030914
CGTGCCG1201.5869715E-514.99580110
GTAGGAC2351.4551915E-1114.4744693
ATTATAC4950.013.7434373
ACGCAGA46400.013.6599677
TTATACC3450.013.3376134
GCGCGTT1050.001469327313.33633236
CGCAGAG48000.013.079678
GTACATG52700.013.01176551
GTCCTAA3400.012.973721
GTATATA6100.012.819041
Produced by FastQC (version 0.11.5)