FastQCFastQC Report
Mon 6 Feb 2017
SRR2077319_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077319_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2080637
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA37800.037.8774871
GGTATCA16500.037.8191261
ATCAACG51250.027.5880763
TCAACGC51200.027.5473334
TATCAAC51750.027.455452
CAACGCA52300.027.0322445
AACGCAG54550.025.916636
ACGCAGA63050.022.0510087
CGCAGAG64950.021.1332478
GTACATG56350.020.6713241
TACATGG56750.020.4566272
GAGTACT42500.019.96316512
AGAGTAC65350.019.89660511
TGCGATA2453.8198777E-1119.70811713
AGTACTT45000.019.23731413
ACATGGG59400.018.9022563
GCACCGT3700.018.7301546
GTGCGAT3001.8189894E-1218.39424512
CAGAGTA71600.018.35161410
GCAGAGT73850.018.0726919
Produced by FastQC (version 0.11.5)