FastQCFastQC Report
Mon 6 Feb 2017
SRR2077318_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077318_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1884594
Sequences flagged as poor quality0
Sequence length46
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45210.2398925179640814No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21650.11487885454373728No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT21450.1138176180121554No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10900.024.1038721
GTATCAA26700.023.8867931
GTCGTAC552.9826406E-421.8791561
GCGATAC1052.1250708E-719.0410514
TATCAAC35900.017.1742732
ATCAACG36700.016.790983
TCAACGC36550.016.7486324
CAACGCA37500.016.37425
AACGCAG39100.015.6974926
GGACCGT904.5947844E-415.5497786
TGCGATA1301.9870822E-615.37890113
GGTGCGA1505.4443626E-714.6612211
GTATTAG2751.8189894E-1213.8567981
GTCTTAT2950.013.5972151
TCTATAC3100.013.5534233
GTGGTAT4650.013.3705951
ATTCGGT1802.9035618E-713.33191826
ACCGTAC900.008220063513.3283818
GTATAAG3650.013.1874361
ACGCAGA46150.013.0828967
Produced by FastQC (version 0.11.5)