FastQCFastQC Report
Mon 6 Feb 2017
SRR2077318_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077318_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1884594
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17000.039.3681951
GTATCAA37450.036.9452131
CAACGCA51100.026.9204525
TCAACGC51250.026.8438074
ATCAACG51750.026.518193
TATCAAC52450.026.1670682
AACGCAG53000.025.9539956
ACGCAGA62250.022.0040937
CGCAGAG64600.021.0895398
GTACATG48950.020.898081
TACATGG50050.019.8046572
AGAGTAC64300.019.74069211
GAGTACT41950.019.24019212
GCAGAGT70450.019.045079
CAGAGTA69050.018.88174410
GTACCGA2405.7480065E-1018.768166
CGAATCG1852.6608905E-718.63819937
ACATGGG52650.018.429723
AGTACTT45600.018.0783413
GTACTTT46550.017.4074614
Produced by FastQC (version 0.11.5)