FastQCFastQC Report
Mon 6 Feb 2017
SRR2077317_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077317_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1638672
Sequences flagged as poor quality0
Sequence length46
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25930.15823789019401077No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGCGT300.009164290526.6576358
GGTATCA8900.025.2354761
GTATCAA21100.024.139861
ATACCGC550.00779178518.1756616
TATCAAC28100.018.0942542
ATCAACG28450.017.4420283
TACGAGT700.001535703117.1501334
TCAACGC28650.017.1102264
CAACGCA29150.016.9498795
AACGCAG30650.016.0467666
ACTATAC1757.385097E-1016.007283
GTAATAG1655.846232E-915.7647463
GATACCG800.00375033315.0027025
GTATAAG3300.014.584141
GACCCGT1101.2715337E-414.5405286
TATAGGG1557.7732875E-714.206082
GTATTAT3600.013.9258261
TACACCG1151.8468912E-413.9155495
GGACCGT1151.8550413E-413.9083326
TAGGACA3050.013.7763374
Produced by FastQC (version 0.11.5)