FastQCFastQC Report
Mon 6 Feb 2017
SRR2077317_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077317_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1638672
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA28300.039.2245981
GGTATCA12500.038.5744481
ATCAACG40300.026.9298733
TCAACGC40650.026.78334
CAACGCA41500.026.4010165
TATCAAC41550.026.2031562
AACGCAG43400.025.3243246
ACGCAGA49400.021.7273857
CGCAGAG52550.020.4762738
GAGTACT34400.020.2523412
AGAGTAC53050.019.76380211
CAGAGTA56050.019.25976610
AGTACTT36350.019.16590113
GTACATG41200.018.8601761
TACCGTC1108.2190277E-418.8248967
GACCCGT1501.6239243E-518.4912836
GCAGAGT59150.018.2503789
GTACTTT37500.017.83747914
TACATGG41850.017.8130382
CGTGCCG1452.7298546E-416.64992710
Produced by FastQC (version 0.11.5)