FastQCFastQC Report
Mon 6 Feb 2017
SRR2077316_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077316_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1671488
Sequences flagged as poor quality0
Sequence length46
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57710.34526122831871964No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT27110.16219081441206876No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26500.1585413715204656No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15700.027.3176291
GTATCAA39600.023.3816781
TAGACGC450.002431108822.2276140
CGGATAA659.376624E-418.45938526
GTACCGA902.2835991E-517.7730456
TATCAAC53250.017.2956772
TCAACGC53250.017.2070774
GTCACGC700.001542838917.13829217
ATCAACG53550.017.1147793
CAACGCA54650.016.6900625
AACGCAG55750.016.3185236
ATATCGT1058.5498796E-515.23403811
TCGGATA1101.269157E-414.54375825
TATCGTA1101.2707734E-414.54158212
AAATTCG850.005620713314.11599921
TAGTACG850.00562629114.1138896
ACGCAGA65350.013.8295237
TAAGGCG1456.135837E-613.7947965
GTACATG74650.013.5846851
CGCAGAG66200.013.5613428
Produced by FastQC (version 0.11.5)