##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2077316_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1671488 Sequences flagged as poor quality 0 Sequence length 46 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.401819217367997 31.0 31.0 34.0 26.0 34.0 2 30.498789701152507 31.0 31.0 34.0 26.0 34.0 3 30.594169985067197 33.0 31.0 34.0 26.0 34.0 4 33.96497312574186 37.0 35.0 37.0 28.0 37.0 5 34.03777831489069 37.0 35.0 37.0 30.0 37.0 6 34.153157545851364 37.0 35.0 37.0 30.0 37.0 7 34.09050379063445 37.0 35.0 37.0 30.0 37.0 8 34.031341535206955 37.0 35.0 37.0 30.0 37.0 9 35.508652769269055 39.0 37.0 39.0 29.0 39.0 10 35.448319700769616 39.0 37.0 39.0 29.0 39.0 11 35.456792989240725 39.0 37.0 39.0 29.0 39.0 12 35.38153250277597 39.0 37.0 39.0 29.0 39.0 13 35.338828636520276 39.0 37.0 39.0 28.0 39.0 14 36.42053547497798 40.0 37.0 41.0 27.0 41.0 15 36.551977040816325 40.0 37.0 41.0 28.0 41.0 16 36.544516622314966 40.0 37.0 41.0 28.0 41.0 17 36.57822969713214 40.0 37.0 41.0 28.0 41.0 18 36.54566170980587 40.0 37.0 41.0 28.0 41.0 19 36.64974023145844 40.0 38.0 41.0 28.0 41.0 20 36.65191254738293 40.0 38.0 41.0 28.0 41.0 21 36.678020422521726 40.0 38.0 41.0 29.0 41.0 22 36.64125976375541 40.0 37.0 41.0 29.0 41.0 23 36.60904714840908 40.0 37.0 41.0 29.0 41.0 24 36.64828464218708 40.0 37.0 41.0 29.0 41.0 25 36.60923799632423 40.0 37.0 41.0 29.0 41.0 26 36.648751890531074 40.0 37.0 41.0 29.0 41.0 27 36.64077696040893 40.0 37.0 41.0 29.0 41.0 28 36.58486450396293 40.0 37.0 41.0 29.0 41.0 29 36.55891038404105 40.0 37.0 41.0 29.0 41.0 30 36.47145357908642 40.0 37.0 41.0 29.0 41.0 31 36.444819825209635 40.0 37.0 41.0 29.0 41.0 32 36.41879869912318 40.0 37.0 41.0 29.0 41.0 33 36.37318006470881 40.0 37.0 41.0 29.0 41.0 34 36.32371754987173 40.0 37.0 41.0 29.0 41.0 35 36.2916132212735 40.0 36.0 41.0 29.0 41.0 36 36.23183235536241 40.0 36.0 41.0 29.0 41.0 37 36.150342688670214 40.0 36.0 41.0 29.0 41.0 38 36.114927537619174 40.0 36.0 41.0 28.0 41.0 39 36.030768393192176 40.0 36.0 41.0 28.0 41.0 40 36.00493332886626 40.0 36.0 41.0 28.0 41.0 41 35.87221685109316 40.0 36.0 41.0 27.0 41.0 42 35.81756016196347 40.0 36.0 41.0 27.0 41.0 43 35.716234875751425 40.0 35.0 41.0 27.0 41.0 44 35.631849585519014 40.0 35.0 41.0 27.0 41.0 45 35.527945160240456 40.0 35.0 41.0 27.0 41.0 46 35.41335205517479 40.0 35.0 41.0 26.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.26548187596879913 1101 2 0.25852367395487263 1101 3 0.41418687136953736 1101 4 0.38712855204566665 1101 5 0.31721279123783575 1101 6 0.43404337885345257 1101 7 0.5438879132161958 1101 8 0.5191709429852764 1101 9 0.5104244316641982 1101 10 0.5416175797590483 1101 11 0.5085654903076957 1101 12 0.49948201734285647 1101 13 0.443645140376006 1101 14 0.6564282511370081 1101 15 0.5580452958489914 1101 16 0.48678915392892463 1101 17 0.5123861079797294 1101 18 0.4120123435764711 1101 19 0.4397613110898675 1101 20 0.3596215207139153 1101 21 0.39153466207446996 1101 22 0.4377537368732902 1101 23 0.47959357112033985 1101 24 0.37167933969592326 1101 25 0.4053525279896135 1101 26 0.4902775365805212 1101 27 0.4043365623982851 1101 28 0.34448880999359943 1101 29 0.4628861369804582 1101 30 0.4939915112401465 1101 31 0.595223802965613 1101 32 0.44598737682166956 1101 33 0.5852386871508344 1101 34 0.47513481351847986 1101 35 0.4668894125346199 1101 36 0.44556820786232265 1101 37 0.5628407225444718 1101 38 0.4240187210554467 1101 39 0.5695874765785831 1101 40 0.5135672121605381 1101 41 0.470474408451544 1101 42 0.43586632193588315 1101 43 0.3918891877418602 1101 44 0.2890023053880526 1101 45 0.30710633077035254 1101 46 0.4285488553072554 1102 1 0.5327069782223077 1102 2 0.5660832070001334 1102 3 0.6520579720799482 1102 4 0.644877713719211 1102 5 0.6337225617351265 1102 6 0.6161888852607262 1102 7 0.6951145302315496 1102 8 0.7512421282899595 1102 9 0.8725676425483755 1102 10 0.7839975412594384 1102 11 0.855595799005954 1102 12 0.7744422884979798 1102 13 0.8940420037279111 1102 14 1.1862078809201506 1102 15 1.0378551429345606 1102 16 1.0762279410425393 1102 17 1.0155350130364695 1102 18 1.045798830172103 1102 19 0.9406587968544358 1102 20 0.9866227527863387 1102 21 0.9354652352538366 1102 22 0.8974910411781778 1102 23 0.9030516916395541 1102 24 0.9366975515542819 1102 25 1.0128331347162032 1102 26 0.8940669563445454 1102 27 0.9559038237046238 1102 28 0.9024470484771996 1102 29 1.0292255144827749 1102 30 0.9422134727362916 1102 31 0.9119092801687358 1102 32 0.8869159159125175 1102 33 0.9793791164845089 1102 34 0.8892620577642631 1102 35 0.861048085608914 1102 36 0.8895893266747734 1102 37 0.868290712377572 1102 38 0.8825530012212042 1102 39 0.9496734949300176 1102 40 0.7914493157948996 1102 41 0.8630552636472544 1102 42 0.858779432844365 1102 43 0.7510719531811958 1102 44 0.8571984705891964 1102 45 0.8216968951785546 1102 46 0.7839250554088508 1103 1 0.286425350517856 1103 2 0.3105631955601744 1103 3 0.35631035484737694 1103 4 0.39412863820057 1103 5 0.42636714166900447 1103 6 0.41226185203186105 1103 7 0.4435115729199808 1103 8 0.32869615112769424 1103 9 0.562199614351762 1103 10 0.47618292825564623 1103 11 0.5074337281265642 1103 12 0.5176548284208096 1103 13 0.5865252205783875 1103 14 0.6710687110475675 1103 15 0.568295771659038 1103 16 0.6595747770247087 1103 17 0.4684940992389244 1103 18 0.48325070287376093 1103 19 0.4328434637093679 1103 20 0.5579540316595057 1103 21 0.6100313372783077 1103 22 0.47169877004292715 1103 23 0.5448441644143571 1103 24 0.5435348814144731 1103 25 0.5173049548166873 1103 26 0.6265138986504297 1103 27 0.5951403093534893 1103 28 0.6155458523739838 1103 29 0.5821499824655518 1103 30 0.6204610398161634 1103 31 0.5757488779595477 1103 32 0.5276034862227377 1103 33 0.5564257451174228 1103 34 0.6781392700768265 1103 35 0.597261366762865 1103 36 0.6327319100169788 1103 37 0.6092214332441301 1103 38 0.5798416178966974 1103 39 0.6389597989232527 1103 40 0.6346951365321942 1103 41 0.6291991591897386 1103 42 0.6042619609311615 1103 43 0.5864347989326006 1103 44 0.6615165405282895 1103 45 0.6208335486717758 1103 46 0.4585914628246641 1104 1 0.3534259576057117 1104 2 0.3464787152012754 1104 3 0.4248796230665377 1104 4 0.4338702427480854 1104 5 0.3639066423750279 1104 6 0.40900328059968416 1104 7 0.3335495222260363 1104 8 0.37072094489885643 1104 9 0.46383774115313514 1104 10 0.4400867136715547 1104 11 0.4581578972118976 1104 12 0.3826384892532033 1104 13 0.4864540708920799 1104 14 0.68744829504503 1104 15 0.5978083247371302 1104 16 0.6618793532546476 1104 17 0.5210167134720649 1104 18 0.5635760884924323 1104 19 0.5016592719066537 1104 20 0.6208746041913429 1104 21 0.4318374952205062 1104 22 0.549968308630902 1104 23 0.4572290092785707 1104 24 0.5408108167381513 1104 25 0.4086412806468829 1104 26 0.3636935250851039 1104 27 0.5518297691800171 1104 28 0.5113172033844364 1104 29 0.5869069915516576 1104 30 0.6336425085646127 1104 31 0.6348186105461835 1104 32 0.5237543302937837 1104 33 0.6103245374251784 1104 34 0.5779088124538347 1104 35 0.7112285132899601 1104 36 0.664770487461098 1104 37 0.5610664835414809 1104 38 0.6006799717034568 1104 39 0.6110125389439816 1104 40 0.5626229461238452 1104 41 0.6272138724396612 1104 42 0.65045738933226 1104 43 0.5776737044230771 1104 44 0.510258033088725 1104 45 0.5240197916062783 1104 46 0.44964714734779676 1105 1 0.28226791291903197 1105 2 0.2634598011477962 1105 3 0.4929247416695297 1105 4 0.5958522536569362 1105 5 0.6899468175229586 1105 6 0.6422266235520837 1105 7 0.6408619402731546 1105 8 0.7097964878354475 1105 9 0.7953615167597192 1105 10 0.6949298897721619 1105 11 0.691578293812924 1105 12 0.7814353047749663 1105 13 0.7120056866423567 1105 14 0.9011528954898296 1105 15 0.6877633968312153 1105 16 0.8347918925655478 1105 17 0.763086445037402 1105 18 0.6358662544722335 1105 19 0.6982001572789684 1105 20 0.7530979284000168 1105 21 0.6463925720412718 1105 22 0.690108006465671 1105 23 0.7639962292655795 1105 24 0.6636549414344799 1105 25 0.6914678677314754 1105 26 0.7898886212363649 1105 27 0.7807797046771441 1105 28 0.9250082757458244 1105 29 0.8185765607799951 1105 30 0.9589172875719569 1105 31 0.9229949841559915 1105 32 0.8439163087189314 1105 33 0.9677558966634336 1105 34 0.885928928550868 1105 35 1.0043261754578623 1105 36 1.025129943306169 1105 37 0.9735088975057238 1105 38 0.9069187814928981 1105 39 0.9216378705246413 1105 40 0.8248702215596992 1105 41 0.7969121065358067 1105 42 0.912918180301368 1105 43 0.8433962303933313 1105 44 0.9080571932066306 1105 45 0.7690595079402982 1105 46 0.8106274281288677 1106 1 0.3097999453502318 1106 2 0.28733394783695587 1106 3 0.3583390187038873 1106 4 0.39340407951240763 1106 5 0.37797810840979906 1106 6 0.4152709390728475 1106 7 0.49431005266959005 1106 8 0.5149562946562938 1106 9 0.29179722556867205 1106 10 0.41780396045120227 1106 11 0.4132097854053782 1106 12 0.4430202600221804 1106 13 0.4178708317236328 1106 14 0.6885029963207359 1106 15 0.6583262493403055 1106 16 0.6853315239018798 1106 17 0.5897467434930022 1106 18 0.6493469491172164 1106 19 0.6372761821474029 1106 20 0.5161525044766648 1106 21 0.5700717665479331 1106 22 0.5481078123076273 1106 23 0.44633097491772844 1106 24 0.48004414955326524 1106 25 0.5864578608127715 1106 26 0.4668465827778405 1106 27 0.5411061819192398 1106 28 0.5675811389011969 1106 29 0.5283611701406485 1106 30 0.6923107375092457 1106 31 0.6666156038702269 1106 32 0.5818147763775983 1106 33 0.6083925276096593 1106 34 0.5595087345581291 1106 35 0.4281049610495202 1106 36 0.3499440385969308 1106 37 0.41715711748322803 1106 38 0.4111075225627232 1106 39 0.44085068443711606 1106 40 0.4739156138634897 1106 41 0.5707614162294021 1106 42 0.5155526683780707 1106 43 0.4078365244439084 1106 44 0.5546931133204822 1106 45 0.5204675184345575 1106 46 0.49436811790207713 1107 1 0.3981107458946944 1107 2 0.30449501544859103 1107 3 0.4264200615625704 1107 4 0.4541720085817431 1107 5 0.42698980667523045 1107 6 0.3644468357118953 1107 7 0.3385768349089986 1107 8 0.4067649501660213 1107 9 0.4238592482032857 1107 10 0.3942128428314007 1107 11 0.4000712304711058 1107 12 0.46998300392294823 1107 13 0.3527817182527002 1107 14 0.7222312628591112 1107 15 0.537933904307657 1107 16 0.6252498606399541 1107 17 0.49609712664886274 1107 18 0.5623654035865968 1107 19 0.5350045712501696 1107 20 0.585133984934437 1107 21 0.38299997193046664 1107 22 0.41411745382890075 1107 23 0.44829500606045514 1107 24 0.5626809770562105 1107 25 0.41115414278414875 1107 26 0.35854088697696085 1107 27 0.43406056174817564 1107 28 0.48977028418200774 1107 29 0.5673571782690701 1107 30 0.48748605986464355 1107 31 0.4465537720317272 1107 32 0.5529503891347858 1107 33 0.48345740302517015 1107 34 0.3917127170794359 1107 35 0.410724193613234 1107 36 0.4934573025592357 1107 37 0.4798810225615284 1107 38 0.4301195335568764 1107 39 0.4326654049887324 1107 40 0.5052175477589529 1107 41 0.6501145921475242 1107 42 0.525466800652211 1107 43 0.46414459445597345 1107 44 0.6214213675392415 1107 45 0.6338547400862495 1107 46 0.5813965727410491 1108 1 0.11547831497835048 1108 2 0.07951750978056538 1108 3 0.09091250404438966 1108 4 0.11585512854938429 1108 5 0.09579073799071125 1108 6 0.025340185077659783 1108 7 0.08411329395929101 1108 8 0.19589574365301132 1108 9 0.0490501955299294 1108 10 0.25114159847321815 1108 11 0.16502209847427451 1108 12 0.0840320778796908 1108 13 0.03859112980219237 1108 14 0.23844435080746962 1108 15 0.15938825517182664 1108 16 0.15900913527865157 1108 17 0.11824370430452547 1108 18 0.20036604111592737 1108 19 0.17630209840821465 1108 20 0.19602063386088986 1108 21 0.1801735716526025 1108 22 0.3136868328516371 1108 23 0.17002302656835866 1108 24 0.2419654369901636 1108 25 0.2198397353810293 1108 26 0.11834290039060136 1108 27 0.16002915507223037 1108 28 0.249317346639522 1108 29 0.2772132662031481 1108 30 0.11657161854909504 1108 31 0.1752136325106548 1108 32 0.28450511288372127 1108 33 0.2011993458608572 1108 34 0.21628275702985889 1108 35 0.22969716364601567 1108 36 0.19382049371866827 1108 37 0.23626611580095158 1108 38 0.04359777870421766 1108 39 0.09310415765561686 1108 40 0.12558615396729778 1108 41 0.18539582371561636 1108 42 0.15087243552415686 1108 43 0.08903926385386995 1108 44 -0.037186083653146795 1108 45 -0.029898982759881676 1108 46 -0.030016691211592672 1109 1 0.2818264949668574 1109 2 0.2515353210994249 1109 3 0.3675196051753531 1109 4 0.3004107536165108 1109 5 0.3070977480784691 1109 6 0.26097873964778984 1109 7 0.31286362044927074 1109 8 0.2364321117200916 1109 9 0.33026002005858146 1109 10 0.28588955718154807 1109 11 0.3545671487066073 1109 12 0.2826976052346879 1109 13 0.3577972078863283 1109 14 0.42145765663934753 1109 15 0.3081312575153774 1109 16 0.3609594585373941 1109 17 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-0.7655026448097928 2215 22 -0.6734537545251627 2215 23 -0.7065035790226801 2215 24 -0.7617755831052477 2215 25 -1.1141162735729324 2215 26 -0.8912553510170582 2215 27 -0.8895569783886756 2215 28 -0.9271926014726333 2215 29 -0.7286918838839611 2215 30 -0.8640889276685613 2215 31 -0.8820232635500318 2215 32 -0.8543410808689273 2215 33 -0.7992299097189672 2215 34 -0.9576319263431472 2215 35 -1.033589390493411 2215 36 -1.1624205642956653 2215 37 -1.117296457192964 2215 38 -0.986036023870696 2215 39 -1.052477778281684 2215 40 -0.8379239003178327 2215 41 -0.8158073804799741 2215 42 -0.9455467165766152 2215 43 -0.781698565817571 2215 44 -0.8227003051267445 2215 45 -0.930825103686729 2215 46 -0.9335183456173652 2216 1 -1.559299233395837 2216 2 -1.5371711282516642 2216 3 -1.3122640821868004 2216 4 -1.2785694177250164 2216 5 -1.2998394616174522 2216 6 -1.604518427494824 2216 7 -1.591991324894984 2216 8 -1.631131469742357 2216 9 -2.044569860743806 2216 10 -2.079768259436271 2216 11 -1.8677674266602935 2216 12 -2.1394123832404546 2216 13 -2.123531723257372 2216 14 -3.9954806544994597 2216 15 -2.916509900450336 2216 16 -2.5282684299965794 2216 17 -2.4193116466356486 2216 18 -2.6342209445344054 2216 19 -2.421046059174472 2216 20 -2.1297342684577956 2216 21 -2.2277564012110744 2216 22 -2.27877556907157 2216 23 -2.3313865210378495 2216 24 -2.27079769546404 2216 25 -2.313833647087037 2216 26 -2.2380945745113365 2216 27 -2.5187881499809137 2216 28 -2.3541703541280583 2216 29 -2.5255298418865095 2216 30 -2.4548938647736236 2216 31 -2.391288350320579 2216 32 -2.2521939632372536 2216 33 -2.149897667520719 2216 34 -2.1718568970259184 2216 35 -2.2762187448395323 2216 36 -2.5907093663811978 2216 37 -2.4347593993768655 2216 38 -2.421888248183322 2216 39 -2.419093262838466 2216 40 -2.39125256539279 2216 41 -2.444861383230311 2216 42 -2.3843525985821614 2216 43 -2.4273456961041617 2216 44 -2.4013497723547275 2216 45 -2.681641345851233 2216 46 -2.792870751317139 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 47359.0 3 1447.0 4 4966.0 5 4077.0 6 3053.0 7 2473.0 8 2184.0 9 1956.0 10 1803.0 11 1760.0 12 1757.0 13 1722.0 14 1711.0 15 1878.0 16 2125.0 17 2208.0 18 2523.0 19 3456.0 20 5205.0 21 7731.0 22 10118.0 23 11613.0 24 11556.0 25 11463.0 26 12260.0 27 14827.0 28 16530.0 29 17151.0 30 18953.0 31 22681.0 32 28764.0 33 35749.0 34 46631.0 35 63447.0 36 88057.0 37 129898.0 38 231299.0 39 799097.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.110304831305584 18.833502853878883 13.88196455232491 23.174227762490627 2 15.07166880852065 21.10002507974341 39.56134990072851 24.266956211007436 3 17.81367502231439 27.15195393807765 29.915405876391173 25.118965163216796 4 12.054513423933637 17.477422255158594 38.97151914835795 31.496545172549823 5 13.12553790244679 39.11297417709155 34.2566203147874 13.504867605674264 6 29.880142723130525 38.37825937129073 18.44518177815216 13.29641612742658 7 27.03112436344144 33.46545114293372 22.376589003331162 17.12683549029368 8 26.295552226519124 34.30482300800245 20.778970593866063 18.62065417161236 9 27.211741873109467 16.006396695638855 19.563107841635716 37.21875358961596 10 14.610335222269018 29.531890148179347 33.75369730443772 22.10407732511391 11 32.99898054906766 24.638705153731287 24.35548445456982 18.006829842631237 12 22.99119108243673 26.260852605582567 30.286547076616767 20.461409235363938 13 27.04661953899759 23.50037810621434 26.62639516406938 22.82660719071869 14 22.262738350499674 22.34571830608416 27.58488245204273 27.806660891373436 15 22.96119917319031 29.926681962446565 24.517180830219836 22.59493803414329 16 22.64347694987939 29.513343799058084 25.609516789830376 22.233662461232147 17 21.455356929188504 29.033311795534495 26.965742934405434 22.545588340871564 18 21.498821697987346 28.62666641937979 28.19680605071096 21.677705831921905 19 22.690549692488094 28.098750807667454 28.276796611386317 20.933902888458135 20 23.196344737183754 27.41961634577819 28.10040126763959 21.283637649398457 21 23.48021179215651 27.27882975859284 27.78216518591642 21.45879326333423 22 23.15762960906689 27.03303882528621 27.727928648007044 22.08140291763985 23 22.811566508982153 27.326185580332652 27.665764672167974 22.19648323851722 24 22.337908328980895 27.317396530134726 27.86284226465575 22.48185287622863 25 22.534342504297115 27.264726698817988 27.890223641331495 22.310707155553402 26 22.256097925777908 27.45726815318253 27.95293962799213 22.333694293047436 27 22.33177147849607 27.611140034348836 27.625202712002345 22.431885775152743 28 22.434509899645462 27.364290440652983 27.797979507907396 22.403220151794166 29 22.49358049331486 27.56899922702678 27.765174086491633 22.172246193166725 30 22.4902899726468 27.556674731433684 27.700919191991712 22.252116103927804 31 22.75615867287599 27.069921936697412 27.79641081112127 22.377508579305328 32 22.384341466683257 27.441919457819214 27.658195889611076 22.515543185886454 33 22.585352282891254 27.2871240924823 27.62826399753508 22.499259627091366 34 22.796861398370872 27.27304817089405 27.687719308741837 22.242371121993244 35 22.667996450983548 27.52318497303836 27.51654402856444 22.29227454741365 36 22.711481804422906 27.36209763953314 27.453062059877333 22.47335849616663 37 22.918415493020948 27.07569109927909 27.65037313946316 22.355520268236805 38 22.648583349139383 27.164586151746455 27.70973032386147 22.477100175252694 39 22.7483173170447 27.490295954677546 27.466234112150932 22.29515261612682 40 22.723157682478575 27.171262724749308 27.77907088761652 22.326508705155597 41 22.49881629283868 27.42848523194375 27.594653175298202 22.47804529991937 42 22.730023542735783 27.337108870637067 27.459521519185863 22.473346067441287 43 22.527815460806426 27.14470622098099 27.508298411288834 22.81917990692375 44 22.55086475621002 27.381243757663597 27.477051839892187 22.590839646234194 45 22.806986047652817 27.473527044454222 27.106331666711547 22.613155241181417 46 22.84162200589307 27.090698670828356 27.297745937445395 22.76993338583318 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1053.0 1 1028.0 2 1003.0 3 1187.0 4 1371.0 5 1400.0 6 1429.0 7 1429.0 8 1358.0 9 1287.0 10 1411.5 11 1536.0 12 2100.0 13 2664.0 14 4698.5 15 6733.0 16 9356.5 17 11980.0 18 12395.0 19 12810.0 20 12810.0 21 14409.5 22 16009.0 23 19598.5 24 23188.0 25 28877.0 26 34566.0 27 39244.5 28 43923.0 29 49500.0 30 55077.0 31 55077.0 32 60122.0 33 65167.0 34 72063.5 35 78960.0 36 83891.5 37 88823.0 38 93244.5 39 97666.0 40 102258.0 41 106850.0 42 109351.5 43 111853.0 44 111853.0 45 112972.5 46 114092.0 47 116134.0 48 118176.0 49 118426.0 50 118676.0 51 115125.5 52 111575.0 53 106709.0 54 101843.0 55 96394.0 56 90945.0 57 90945.0 58 83111.5 59 75278.0 60 67744.0 61 60210.0 62 52020.0 63 43830.0 64 36820.0 65 29810.0 66 24127.0 67 18444.0 68 14846.0 69 11248.0 70 11248.0 71 8636.0 72 6024.0 73 4757.5 74 3491.0 75 2682.0 76 1873.0 77 1389.5 78 906.0 79 683.0 80 460.0 81 460.0 82 383.0 83 306.0 84 236.5 85 167.0 86 132.5 87 98.0 88 69.5 89 41.0 90 24.5 91 8.0 92 6.5 93 5.0 94 5.0 95 8.0 96 11.0 97 10.5 98 10.0 99 11.5 100 13.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.18289093310870314 2 0.04887860397442279 3 0.062279836887850826 4 0.05545956656583834 5 0.038648198491404065 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 1.7948079794769691E-4 16 0.0 17 4.7861546119385844E-4 18 8.974039897384845E-4 19 4.7861546119385844E-4 20 0.0020939426427231305 21 0.002273423440670827 22 0.0 23 4.187885285446261E-4 24 6.580962591415553E-4 25 0.0010170578550369493 26 0.0010768847876861814 27 0.023811119194394458 28 8.375770570892522E-4 29 0.001196538652984646 30 0.001196538652984646 31 0.0014358463835815753 32 9.572309223877169E-4 33 0.0019742887774246657 34 0.0025725581039169887 35 0.00233325037332006 36 0.03021260098786231 37 0.007717674311750968 38 0.01118763640540644 39 0.04744275759084121 40 0.014178983037868055 41 0.05318614312516751 42 0.05438268177815216 43 0.023033369069954436 44 0.02626402343301298 45 0.026503331163609912 46 0.022255618945514415 >>END_MODULE >>Sequence Length Distribution pass #Length Count 46 1671488.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.25022949243256 #Duplication Level Percentage of deduplicated Percentage of total 1 76.8898813052763 47.09522875594073 2 13.1111805919294 16.06125640344807 3 4.475644744555874 8.22402803191941 4 1.9769931780507142 4.843651434423194 5 1.0368309472481125 3.175306673190157 6 0.6237042674621062 2.292121771047813 7 0.4125379740591841 1.768763190982775 8 0.2639410617621397 1.29331604843259 9 0.19277634678813935 1.062683593333767 >10 0.9581137085117409 10.23973145457092 >50 0.04446867087127098 1.8624271799239855 >100 0.013534951532587545 1.3645638831557525 >500 9.806298810911895E-5 0.048291299432233654 >1k 1.961259762182379E-4 0.322002060020155 >5k 9.806298810911895E-5 0.34662822017838363 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5771 0.34526122831871964 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2711 0.16219081441206876 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2650 0.1585413715204656 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.98269326492323E-5 2 0.0 0.0 0.0 0.0 5.98269326492323E-5 3 0.0 0.0 0.0 0.0 5.98269326492323E-5 4 0.0 0.0 0.0 5.98269326492323E-5 5.98269326492323E-5 5 0.0 0.0 0.0 5.98269326492323E-5 5.98269326492323E-5 6 0.0 0.0 0.0 5.98269326492323E-5 5.98269326492323E-5 7 0.0 0.0 0.0 5.98269326492323E-5 5.98269326492323E-5 8 0.0 0.0 0.0 5.98269326492323E-5 5.98269326492323E-5 9 0.0 0.0 0.0 5.98269326492323E-5 5.98269326492323E-5 10 5.98269326492323E-5 0.0 0.0 1.196538652984646E-4 5.98269326492323E-5 11 5.98269326492323E-5 0.0 0.0 1.196538652984646E-4 5.98269326492323E-5 12 5.98269326492323E-5 0.0 0.0 1.196538652984646E-4 5.98269326492323E-5 13 5.98269326492323E-5 0.0 0.0 1.196538652984646E-4 5.98269326492323E-5 14 5.98269326492323E-5 0.0 0.0 1.196538652984646E-4 5.98269326492323E-5 15 1.7948079794769691E-4 0.0 0.0 1.196538652984646E-4 2.393077305969292E-4 16 1.7948079794769691E-4 0.0 0.0 1.196538652984646E-4 2.393077305969292E-4 17 1.7948079794769691E-4 0.0 0.0 1.196538652984646E-4 2.393077305969292E-4 18 1.7948079794769691E-4 0.0 0.0 1.196538652984646E-4 2.393077305969292E-4 19 1.7948079794769691E-4 0.0 0.0 1.196538652984646E-4 2.393077305969292E-4 20 1.7948079794769691E-4 0.0 0.0 1.196538652984646E-4 2.991346632461615E-4 21 1.7948079794769691E-4 0.0 0.0 1.196538652984646E-4 2.991346632461615E-4 22 1.7948079794769691E-4 0.0 0.0 1.196538652984646E-4 2.991346632461615E-4 23 1.7948079794769691E-4 0.0 0.0 1.7948079794769691E-4 2.991346632461615E-4 24 1.7948079794769691E-4 0.0 0.0 3.5896159589539383E-4 2.991346632461615E-4 25 2.991346632461615E-4 0.0 0.0 3.5896159589539383E-4 2.991346632461615E-4 26 2.991346632461615E-4 0.0 0.0 4.786154611938584E-4 2.991346632461615E-4 27 2.991346632461615E-4 0.0 0.0 8.375770570892522E-4 2.991346632461615E-4 28 2.991346632461615E-4 0.0 0.0 0.001615327181529272 2.991346632461615E-4 29 2.991346632461615E-4 0.0 0.0 0.0025127311712677567 2.991346632461615E-4 30 2.991346632461615E-4 0.0 0.0 0.003948577554849332 2.991346632461615E-4 31 2.991346632461615E-4 0.0 0.0 0.006401481793467856 2.991346632461615E-4 32 2.991346632461615E-4 0.0 0.0 0.009153520695332542 2.991346632461615E-4 33 3.5896159589539383E-4 0.0 0.0 0.01220469426044339 2.991346632461615E-4 34 3.5896159589539383E-4 0.0 0.0 0.01561482942144963 2.991346632461615E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1570 0.0 27.317629 1 GTATCAA 3960 0.0 23.381678 1 TAGACGC 45 0.0024311088 22.22761 40 CGGATAA 65 9.376624E-4 18.459385 26 GTACCGA 90 2.2835991E-5 17.773045 6 TATCAAC 5325 0.0 17.295677 2 TCAACGC 5325 0.0 17.207077 4 GTCACGC 70 0.0015428389 17.138292 17 ATCAACG 5355 0.0 17.114779 3 CAACGCA 5465 0.0 16.690062 5 AACGCAG 5575 0.0 16.318523 6 ATATCGT 105 8.5498796E-5 15.234038 11 TCGGATA 110 1.269157E-4 14.543758 25 TATCGTA 110 1.2707734E-4 14.541582 12 AAATTCG 85 0.0056207133 14.115999 21 TAGTACG 85 0.005626291 14.113889 6 ACGCAGA 6535 0.0 13.829523 7 TAAGGCG 145 6.135837E-6 13.794796 5 GTACATG 7465 0.0 13.584685 1 CGCAGAG 6620 0.0 13.561342 8 >>END_MODULE