FastQCFastQC Report
Mon 6 Feb 2017
SRR2077316_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077316_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1671488
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23060.1379609066891297No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24700.046.1615941
GTATCAA56850.039.8074721
TCGACGG400.002598703634.49449526
TATCAAC81100.027.807332
ATCAACG81500.027.5850123
TCAACGC81750.027.4582794
CAACGCA83400.026.914235
AACGCAG87000.025.9199856
GTATACG750.001941690623.1041831
ACGCAGA100950.022.15077
GAGTACT65700.021.62866212
CGCAGAG103700.021.5516768
GTGGTAT8050.021.0951231
AGAGTAC102950.020.90528311
AGTACTT68850.020.5890213
CGTATAC850.00402499820.3768543
CAGAGTA107700.020.30291410
GTACATG74400.020.1695791
GCAGAGT108800.020.1610459
CTCGTAT2402.910383E-1120.11576839
Produced by FastQC (version 0.11.5)