FastQCFastQC Report
Mon 6 Feb 2017
SRR2077315_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077315_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1962417
Sequences flagged as poor quality0
Sequence length46
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41090.20938465168208387No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11450.025.9091951
GTATCAA29300.024.2861351
TCAACGC38800.018.0961634
ATCAACG39000.018.0074923
CAACGCA39800.017.7392925
TATCAAC39650.017.6730922
TGCGATA1155.521488E-717.38568713
AACGCAG40800.017.1513216
GTCGAAA750.002406672316.035691
GCGATAC1402.4896508E-715.70921114
ACGCAGA46600.014.7162757
CGATACT1557.87757E-714.18968815
CGCAGAG48450.014.1130898
GTCTAAG2751.8189894E-1213.8490061
AGAGTAC47850.013.41259611
TATCGCA900.008143146513.3473912
GTAATAC1702.210647E-612.9465643
GAGTACT24200.012.80577912
CAGAGTA51550.012.68261410
GTATAAA5850.012.6777891
Produced by FastQC (version 0.11.5)