FastQCFastQC Report
Mon 6 Feb 2017
SRR2077315_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077315_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1962417
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18700.038.9476171
GTATCAA39200.038.1324231
ATCAACG53650.027.8511943
TATCAAC54400.027.470032
TCAACGC54350.027.4286984
TATACCG1155.3867552E-827.1298585
CAACGCA55550.026.7093055
AACGCAG58700.025.4518786
ACGCAGA68200.021.9577587
GCGTAAC800.002911439221.54807363
CGCAGAG69650.021.4390668
AGAGTAC70850.020.10411
CTAGTAC2603.6379788E-1220.0011143
GAGTACT48600.019.44404212
GTACATG50450.019.2486421
TACATGG51550.018.8325942
GTACTTT50550.018.75837714
CAGAGTA76650.018.71678710
GCAGAGT77950.018.713869
ATACCGT1501.6270185E-518.487556
Produced by FastQC (version 0.11.5)