FastQCFastQC Report
Mon 6 Feb 2017
SRR2077314_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077314_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2145480
Sequences flagged as poor quality0
Sequence length46
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58150.2710349199246789No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26110.12169770867125305No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT26010.1212316125062923No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13750.025.6543061
GTATCAA33100.023.1305371
TCAACGC41850.017.3504224
ATCAACG42450.017.1075783
TATCAAC43600.016.941932
CAACGCA43550.016.6711925
AACGCAG44450.016.2821946
CCCCTAT1801.0750227E-915.5885541
CCCCGTA956.910234E-414.73608725
GTATTAG3550.014.1143861
GTATAGG4550.014.0957721
GTATAGA5550.014.0838681
ACGCAGA52750.013.530757
GTCCTAT3300.013.3616181
CGTACAC1202.646928E-413.3373133
CGCGGGA900.00821581813.32954220
CGCAGAG53100.013.3286118
TAGCCCT8200.012.9288794
TAGGACT4700.012.7679824
CCCTATA2201.8428182E-812.7387952
Produced by FastQC (version 0.11.5)