FastQCFastQC Report
Mon 6 Feb 2017
SRR2077314_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077314_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2145480
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA43450.040.9507331
GGTATCA18200.038.305291
ATCAACG60750.028.9879443
TCAACGC61100.028.7084224
TATCAAC62600.028.189292
CAACGCA62250.028.1223775
AACGCAG64350.027.312376
ACGCAGA73950.023.6553347
CGCAGAG75900.022.9475738
AGAGTAC76550.021.53674111
GAGTACT55850.020.9350412
CAGAGTA82600.020.20935610
GCAGAGT84850.019.9986369
GTACATG50100.019.9383351
AGTACTT59100.019.72543113
TACATGG51450.019.0696072
GTACTTT62100.018.65664714
CCGTATA1501.625197E-518.4901082
CGTACAC1954.3723594E-717.7772833
ACATGGG52950.017.7420313
Produced by FastQC (version 0.11.5)