FastQCFastQC Report
Mon 6 Feb 2017
SRR2077313_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077313_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1856918
Sequences flagged as poor quality0
Sequence length46
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37370.20124744334429415No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12250.023.5549961
GTATCAA27550.022.692881
ATCAACG35750.017.2380123
TCAACGC35750.017.236624
CAACGCA35950.016.9696565
TATCAAC36350.016.959422
AACGCAG37250.016.3166966
GTCGCGT1453.7061363E-715.16775616
TCGCGTC1606.904884E-814.99539617
CCGATCA956.924438E-414.7319229
ACGCAGA42250.014.3857287
ACCGACC1151.8551761E-413.9083998
CGCAGAG44000.013.8135678
CCCTATA1508.651454E-613.3436132
GTGTAGC6400.013.1500071
AGAGTAC42900.012.86283711
GAGTACT21350.012.82943912
GTGTTAG3005.456968E-1212.69081
TGACCCG1905.585207E-712.6328055
GTGCGCC1601.700402E-512.49582711
Produced by FastQC (version 0.11.5)