FastQCFastQC Report
Mon 6 Feb 2017
SRR2077313_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077313_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1856918
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15400.039.8474431
GTATCAA35350.038.7396431
ATCAACG46600.028.6998063
TCAACGC46700.028.638354
CAACGCA47250.028.3042285
TATCAAC48400.027.6354482
AACGCAG50000.026.8160636
ACGCAGA56500.023.4518897
CGCAGAG59000.022.506348
GAGTACT39450.021.5958112
AGAGTAC58450.021.06702811
CGCTACG2151.4006218E-1020.85175328
AGTACTT42500.020.12715113
CAGAGTA63850.019.87901110
GCAGAGT66200.019.7459229
GTACTTT43700.019.41137514
CGCTATC2556.730261E-1118.93122533
ACGCTAT2701.5279511E-1017.87949232
GTACATG44500.017.7632641
TCGTATG2451.5981641E-816.88953840
Produced by FastQC (version 0.11.5)