FastQCFastQC Report
Mon 6 Feb 2017
SRR2077312_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077312_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2342020
Sequences flagged as poor quality0
Sequence length46
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52490.22412276581754215No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT24320.10384198256206181No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23430.10004184421994687No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14600.025.6696991
GTATCAA33750.023.634221
TATCAAC44800.017.3791182
ATCAACG45050.017.052883
TCAACGC45300.016.8694
CAACGCA45950.016.5850665
AACGCAG48700.015.7236316
ACCGTCG904.59422E-415.5502928
GCGATAC850.00562987414.1128714
GTATAGT3650.013.7271111
GTCCTAA3300.013.3610541
ACGCAGA56300.013.245967
GTATAAT5750.012.8963231
GTACATG60650.012.8213251
CGCAGAG58300.012.6886738
GTAGGAC2101.3918361E-712.3846943
TTATACT6300.012.3836364
CTTAGAC3900.012.3114133
TACATGG61800.012.24222
AGAGTAC56300.012.03855411
Produced by FastQC (version 0.11.5)