FastQCFastQC Report
Mon 6 Feb 2017
SRR2077312_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077312_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2342020
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA45700.037.8275381
GGTATCA21950.034.722571
ATCAACG63100.026.6753143
TCAACGC63250.026.5595724
CAACGCA63250.026.5025395
TATCAAC64900.026.2573532
AACGCAG67650.024.8811876
ACGCAGA77150.021.3337677
CGCAGAG79800.020.4859688
GTACATG63050.020.3850861
AGAGTAC79650.019.66021211
GAGTACT50800.019.5545112
TACATGG65200.019.336792
TATACCG1108.028217E-418.8912725
AGTACTT53900.018.74981513
CAGAGTA85900.018.67024810
ACATGGG66700.018.3814643
GTACTTT55550.018.31511714
GCAGAGT89050.018.2030859
CCTATAC4300.016.9142783
Produced by FastQC (version 0.11.5)