FastQCFastQC Report
Mon 6 Feb 2017
SRR2077311_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077311_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1099032
Sequences flagged as poor quality0
Sequence length46
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79360.7220899846410296No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT37970.3454858457260571No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36590.33292934145684566No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA32230.29325806709904717No Hit
GAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAG20230.1840710734537302No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGC18900.17196951499137422No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAAT17380.15813916246296741No Hit
GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATT16820.15304376942618594No Hit
TTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCT15140.13775759031584156No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGC14910.13566483960430634No Hit
CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAG14350.1305694465675249No Hit
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGA13230.12037866049396195No Hit
GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAAT12740.11592019158677819No Hit
CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCAT12490.11364546255250076No Hit
ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCC12330.11218963597056318No Hit
TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACC11410.10381863312442222No Hit
ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGA11340.10318170899482454No Hit
GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAG11310.10290874151071125No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGCG250.003761398831.9861728
CGCGGCA355.6061527E-428.58639137
ACGCGGC355.6061527E-428.58639136
GGTATCA16000.027.440571
CCGGTCC457.812492E-526.6551429
CAACGCG400.001223756225.00967634
CGCGTTA651.35558E-624.62827739
AGTTACG602.0322737E-523.34555238
GTATCAA42550.023.0397781
TCGCGTT702.5825902E-622.86911438
CTCGGGT450.002427258322.23284735
CCAACGC450.002430470222.22778733
CCCAACG450.002434329122.22171632
GCGGCAA553.0275996E-421.82960738
ATGCGTA2650.021.12294413
GGTCGGT2250.020.43560811
AACGCGG500.0044709720.00956335
TAGTCGG2400.020.0068340
CGACTAA1101.5697879E-820.00500733
CCGACTA1101.5746991E-819.99954432
Produced by FastQC (version 0.11.5)