##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2077311_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1099032
Sequences flagged as poor quality	0
Sequence length	46
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.05717030987269	31.0	30.0	34.0	26.0	34.0
2	30.040240866507983	31.0	30.0	34.0	25.0	34.0
3	30.21328314371192	31.0	31.0	34.0	26.0	34.0
4	33.462456052235055	37.0	35.0	37.0	27.0	37.0
5	33.645173206967584	37.0	35.0	37.0	28.0	37.0
6	33.63200889510041	37.0	35.0	37.0	28.0	37.0
7	33.516563666935994	37.0	35.0	37.0	27.0	37.0
8	33.320122616993864	37.0	35.0	37.0	26.0	37.0
9	34.62521018496277	39.0	35.0	39.0	24.0	39.0
10	34.641151486034985	39.0	35.0	39.0	25.0	39.0
11	34.606723917047	39.0	35.0	39.0	25.0	39.0
12	34.5777502383916	39.0	35.0	39.0	25.0	39.0
13	34.490337860954	39.0	35.0	39.0	23.0	39.0
14	35.50193897902882	40.0	36.0	41.0	21.0	41.0
15	35.65030863523537	40.0	36.0	41.0	25.0	41.0
16	35.649877346610474	40.0	36.0	41.0	24.0	41.0
17	35.705077741139476	40.0	36.0	41.0	25.0	41.0
18	35.72510172588241	40.0	36.0	41.0	25.0	41.0
19	35.86369914615771	40.0	36.0	41.0	25.0	41.0
20	35.881996156617824	40.0	36.0	41.0	25.0	41.0
21	35.92109419925898	40.0	36.0	41.0	26.0	41.0
22	35.888197067965265	40.0	36.0	41.0	26.0	41.0
23	35.88413258212682	40.0	36.0	41.0	26.0	41.0
24	35.96391278870861	40.0	36.0	41.0	27.0	41.0
25	35.94356761222603	40.0	36.0	41.0	27.0	41.0
26	35.99791361852976	40.0	36.0	41.0	27.0	41.0
27	36.011743971058166	40.0	36.0	41.0	27.0	41.0
28	35.96066993499734	40.0	36.0	41.0	27.0	41.0
29	35.946910554014806	40.0	36.0	41.0	27.0	41.0
30	35.8623843527759	40.0	36.0	41.0	27.0	41.0
31	35.81693071721296	40.0	36.0	41.0	27.0	41.0
32	35.767203320740435	40.0	35.0	41.0	27.0	41.0
33	35.720013611978544	40.0	35.0	41.0	27.0	41.0
34	35.68612560871749	40.0	35.0	41.0	27.0	41.0
35	35.6560973656818	40.0	35.0	41.0	27.0	41.0
36	35.58074378180071	40.0	35.0	41.0	27.0	41.0
37	35.511899562524114	40.0	35.0	41.0	26.0	41.0
38	35.45070025258591	40.0	35.0	41.0	26.0	41.0
39	35.39303587156698	40.0	35.0	41.0	25.0	41.0
40	35.36258725860576	40.0	35.0	41.0	25.0	41.0
41	35.24589365914732	40.0	35.0	41.0	25.0	41.0
42	35.16513986853886	40.0	35.0	41.0	25.0	41.0
43	35.061450440023584	40.0	35.0	41.0	24.0	41.0
44	34.965647952015956	40.0	35.0	41.0	24.0	41.0
45	34.84461871901819	40.0	35.0	41.0	23.0	41.0
46	34.71978977864157	40.0	35.0	41.0	22.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.16370137954690023
1101	2	0.25394019631059805
1101	3	0.2626135426207412
1101	4	0.31480507140316405
1101	5	0.23647272078289205
1101	6	0.17123369136020727
1101	7	0.28933947808641136
1101	8	0.33076554119681134
1101	9	0.32140742267122846
1101	10	0.3251691583841634
1101	11	0.42314391626192815
1101	12	0.24637344121906324
1101	13	0.4488375376676004
1101	14	0.42075105578823013
1101	15	0.32771109454581193
1101	16	0.23017719073396137
1101	17	0.44294226163976447
1101	18	0.35314404352930495
1101	19	0.31016037006313013
1101	20	0.4175962967314746
1101	21	0.2983146141712112
1101	22	0.5146159800472248
1101	23	0.48533064030900164
1101	24	0.35791547547157165
1101	25	0.3655852969999458
1101	26	0.38277510041929474
1101	27	0.3613375914131609
1101	28	0.2305254227806799
1101	29	0.4524522683492336
1101	30	0.380399424800693
1101	31	0.5495713519955174
1101	32	0.3728964133447761
1101	33	0.39799646513674247
1101	34	0.3722665983149085
1101	35	0.316950883020894
1101	36	0.21401061384524667
1101	37	0.17573953360278693
1101	38	0.27216330037323644
1101	39	0.331803904075862
1101	40	0.3004824719180448
1101	41	0.16317909373825756
1101	42	0.20016948916863697
1101	43	0.18411117975166746
1101	44	0.11164571164839288
1101	45	0.17991528962102166
1101	46	0.2238620117078014
1102	1	0.7881355062837265
1102	2	0.8065804481392611
1102	3	0.8997371156604075
1102	4	0.9091279000323382
1102	5	0.8302156377954972
1102	6	0.7997578285476905
1102	7	0.8416758888990685
1102	8	0.8485335022234892
1102	9	1.0604917095575885
1102	10	0.8534114666937711
1102	11	0.8706817218028675
1102	12	1.050148502487545
1102	13	1.019025016559027
1102	14	1.3541972132407665
1102	15	1.188139845256913
1102	16	1.1162020967127333
1102	17	1.039997617470668
1102	18	1.0625054097357278
1102	19	0.9463732368317039
1102	20	0.9941530020820579
1102	21	0.7442200910280405
1102	22	1.030806744832212
1102	23	1.0791225639370907
1102	24	1.0227780180409667
1102	25	0.9916060129130884
1102	26	1.0061866224463358
1102	27	0.9366046059995767
1102	28	0.9674717960665191
1102	29	0.9530491280869882
1102	30	1.0906633295089634
1102	31	1.0222560854954992
1102	32	0.9567730734949222
1102	33	1.1244537891550053
1102	34	1.1621425666549996
1102	35	0.9297406295300874
1102	36	0.9974491548529585
1102	37	1.0672139752234528
1102	38	1.0126426422470658
1102	39	1.02830879309834
1102	40	1.0372385361804177
1102	41	1.1686331216532437
1102	42	1.1212504607574587
1102	43	1.0340659915683048
1102	44	1.1213743415990294
1102	45	0.9088209024712128
1102	46	0.918046437487078
1103	1	0.8358545219838298
1103	2	0.6878628418316701
1103	3	0.8163786919130303
1103	4	0.8020624787947881
1103	5	0.8019372652601149
1103	6	0.8950199318682479
1103	7	0.8736164779472091
1103	8	0.9360205290035211
1103	9	1.1772481310308862
1103	10	1.0589072709861753
1103	11	0.9755722469147301
1103	12	1.045929377176769
1103	13	1.0055867875841003
1103	14	1.265344874361567
1103	15	1.2014190698824407
1103	16	1.2099800282471875
1103	17	1.1650057349575675
1103	18	1.066065690291822
1103	19	1.043517809962097
1103	20	1.0483892282318976
1103	21	0.9523111296767226
1103	22	0.9872183000403751
1103	23	0.9883149438504333
1103	24	1.0581065017416407
1103	25	1.1141432657823458
1103	26	1.0901814571664303
1103	27	1.0184639021062125
1103	28	1.0744962854913496
1103	29	1.0908200996389823
1103	30	1.248198856551312
1103	31	1.2562313792107744
1103	32	1.2208383660334121
1103	33	1.2344796824623003
1103	34	1.1927083978010415
1103	35	1.2915955648243695
1103	36	1.3073927122446705
1103	37	1.3703412050725063
1103	38	1.394011032103684
1103	39	1.4943961959000447
1103	40	1.3402327376381464
1103	41	1.4145947457173733
1103	42	1.4686792935199122
1103	43	1.3195543651624106
1103	44	1.2549202842134193
1103	45	1.2164667682990924
1103	46	1.1833865198466071
1104	1	0.6787520092567974
1104	2	0.6530978308091058
1104	3	0.6774577094119998
1104	4	0.5813991627398565
1104	5	0.5407459941560688
1104	6	0.5989995268977211
1104	7	0.7219841073449871
1104	8	0.6963872807681497
1104	9	0.8091517180859569
1104	10	0.7476445569906716
1104	11	0.7670126970725448
1104	12	0.7961403838242944
1104	13	0.9501780836419798
1104	14	0.9538113765492753
1104	15	0.9785663063616923
1104	16	0.9925780927416383
1104	17	1.0064361224642724
1104	18	0.8093112564482468
1104	19	0.744398262962946
1104	20	1.017730010631027
1104	21	0.9469456700260253
1104	22	0.9485993515854858
1104	23	0.9410100540200474
1104	24	1.032333753873047
1104	25	0.7790125614083578
1104	26	0.8084863272964284
1104	27	0.9055231010110276
1104	28	0.9386864197669027
1104	29	1.1024545329881192
1104	30	1.0923297428642158
1104	31	1.0311377926926397
1104	32	1.0900165622578086
1104	33	1.147180293290326
1104	34	1.1711754832748582
1104	35	0.9858358269400185
1104	36	1.0399440559079665
1104	37	1.0987774845414862
1104	38	0.9770800518469542
1104	39	1.0275807333329112
1104	40	0.9979940546441028
1104	41	1.1975879768945177
1104	42	1.0462758534452234
1104	43	1.0448675531068474
1104	44	1.101374271630661
1104	45	1.0884238786147193
1104	46	1.1520404925078722
1105	1	-0.031132344807915047
1105	2	-0.015441723521675499
1105	3	0.11534054307000119
1105	4	0.051828582609672935
1105	5	0.1620423309261554
1105	6	0.31247584181195975
1105	7	0.15359146231244125
1105	8	0.2715552381967683
1105	9	0.2886127339076836
1105	10	0.20819057304996846
1105	11	0.2977536102668239
1105	12	0.45304882683279857
1105	13	0.5034829726997998
1105	14	0.6411697649181676
1105	15	0.4321032474934441
1105	16	0.4879087336880872
1105	17	0.30756483439471793
1105	18	0.17948657834685378
1105	19	0.35794432653495534
1105	20	0.23144864036447643
1105	21	0.2309002478755957
1105	22	0.40255288415544754
1105	23	0.423907189003657
1105	24	0.34226410024070475
1105	25	0.32131274670792465
1105	26	0.4181914634203423
1105	27	0.3215983611431099
1105	28	0.44714919355889293
1105	29	0.375858248482011
1105	30	0.4239787189653228
1105	31	0.34536708902940916
1105	32	0.38019796578325327
1105	33	0.3400556674529156
1105	34	0.2520805053682693
1105	35	0.46634478308578764
1105	36	0.6041932125573695
1105	37	0.4507964896253469
1105	38	0.4466789495520942
1105	39	0.6172229626292491
1105	40	0.3121651954242637
1105	41	0.2702045018149306
1105	42	0.38189005024286615
1105	43	0.38765692926497053
1105	44	0.28490902717776834
1105	45	0.31924100219527674
1105	46	0.41884868369901795
1106	1	-0.1830698980296006
1106	2	-0.19752101200687022
1106	3	-0.25695011829814973
1106	4	-0.0948887984102953
1106	5	-0.008839304044045093
1106	6	-0.14999667214394918
1106	7	-0.16664507851450594
1106	8	-0.13227861552638842
1106	9	-0.26548245012107685
1106	10	0.0016124672689130648
1106	11	-0.1960687002639716
1106	12	-0.18537524356175084
1106	13	-0.2554550536035407
1106	14	0.012513884513211337
1106	15	-0.03599061190669062
1106	16	0.004405280192017358
1106	17	-0.16296331396369368
1106	18	-0.19625000978825824
1106	19	-0.1754370718944145
1106	20	-0.1714818166324008
1106	21	-0.21695517837097356
1106	22	-0.1911380989082332
1106	23	-0.12305770833762608
1106	24	-0.14566361288257923
1106	25	-0.1831989384089283
1106	26	-0.1528302037194038
1106	27	-0.15650477065744184
1106	28	-0.17367888856808378
1106	29	-0.003227379297342736
1106	30	0.022168519324864633
1106	31	-0.018230980797440566
1106	32	-0.124844706181932
1106	33	-0.06407868773678871
1106	34	-0.020983739120332245
1106	35	-0.25639568431888904
1106	36	-0.3910385028367074
1106	37	-0.33826472714066824
1106	38	-0.26792943345481746
1106	39	-0.08576691342335607
1106	40	-0.15261103695344502
1106	41	-0.010590275363604462
1106	42	-0.1664535962705216
1106	43	-0.11019300923441477
1106	44	-0.00986928675921206
1106	45	-0.0462066276656401
1106	46	-0.2024138766188912
1107	1	0.4175190676471665
1107	2	0.2796375650194669
1107	3	0.39343361498921325
1107	4	0.4680778661469489
1107	5	0.3315671455556384
1107	6	0.3505004972275074
1107	7	0.5282191291459029
1107	8	0.5241668016776373
1107	9	0.5037051234814456
1107	10	0.577122565953573
1107	11	0.6011036534820349
1107	12	0.4405606314090633
1107	13	0.457833516301676
1107	14	0.6329885339669801
1107	15	0.6485665145634556
1107	16	0.45854349691421703
1107	17	0.439321919414958
1107	18	0.6392454829549621
1107	19	0.5889566542427289
1107	20	0.6567088869186364
1107	21	0.5975159054291623
1107	22	0.44895055508298043
1107	23	0.5379906171071909
1107	24	0.5542981265337446
1107	25	0.46313651962336166
1107	26	0.4029571459932413
1107	27	0.5852792054318812
1107	28	0.6359212446893139
1107	29	0.6490970238993796
1107	30	0.628199071055576
1107	31	0.5459049592018133
1107	32	0.5705235810313951
1107	33	0.5700482981220745
1107	34	0.40219140924853747
1107	35	0.4925919740970244
1107	36	0.5484352177431973
1107	37	0.4857500446451368
1107	38	0.5579070803804598
1107	39	0.5740168488140256
1107	40	0.5156387886553944
1107	41	0.5737099378336765
1107	42	0.5344348348491508
1107	43	0.4915812344098569
1107	44	0.6276473249533225
1107	45	0.6111523882663334
1107	46	0.7308457723584354
1108	1	0.20961806388735482
1108	2	0.273434165666675
1108	3	0.07687105161946661
1108	4	0.2452544651705324
1108	5	0.17754465054794366
1108	6	0.17191542118720804
1108	7	0.05552403863904232
1108	8	0.210942858043893
1108	9	0.23277285408618553
1108	10	0.22143813192418094
1108	11	0.098564108212301
1108	12	-0.12305367835873682
1108	13	0.09403164057414415
1108	14	0.30311259176392014
1108	15	0.27874805816812653
1108	16	0.2102020222490424
1108	17	0.3327588431430044
1108	18	0.42995624653048026
1108	19	0.39110581639489084
1108	20	0.39564675815708483
1108	21	0.17995304688589187
1108	22	0.4165572141161249
1108	23	0.2565717054177412
1108	24	0.35951077544946486
1108	25	0.30524382080327683
1108	26	0.16326741446527393
1108	27	0.19644022921185922
1108	28	0.34714371483313755
1108	29	0.5432248370915644
1108	30	0.2132764700367673
1108	31	0.2762036551054976
1108	32	0.33819416602184305
1108	33	0.36176414622285336
1108	34	0.3736855952812306
1108	35	0.34031606395884495
1108	36	0.32238664692312113
1108	37	0.45736141694386845
1108	38	0.3515756932449321
1108	39	0.4000268330838921
1108	40	0.501577116071104
1108	41	0.4646974987662915
1108	42	0.4505946850681184
1108	43	0.4947999505171268
1108	44	0.3320621222159872
1108	45	0.1795108736219575
1108	46	0.17527655450712842
1109	1	0.24501901995635578
1109	2	0.08492552213589022
1109	3	0.21542561928411175
1109	4	0.3177080579730145
1109	5	0.2693333624752512
1109	6	0.20025570710795648
1109	7	0.22374566295051324
1109	8	0.3226169184519918
1109	9	0.29240110682508913
1109	10	0.3536652274278467
1109	11	0.20385180239727418
1109	12	0.3524688906399618
1109	13	0.3158743232846959
1109	14	0.568828042124828
1109	15	0.3765800287766794
1109	16	0.393666620962108
1109	17	0.391237999938852
1109	18	0.4161260678895715
1109	19	0.39661075687880043
1109	20	0.2773550565289611
1109	21	0.33306933685113904
1109	22	0.18486234888426623
1109	23	0.1770716696842669
1109	24	0.33643389818469416
1109	25	0.3931468480904172
1109	26	0.43868880478865435
1109	27	0.4092009843263753
1109	28	0.318245258783989
1109	29	0.41177494468902864
1109	30	0.5437277441337045
1109	31	0.37799310817155884
1109	32	0.35420044717282906
1109	33	0.24581993544299507
1109	34	0.49789085630764873
1109	35	0.43204985221021275
1109	36	0.4029308942305363
1109	37	0.5109907469808945
1109	38	0.5740933416116434
1109	39	0.5850623812751223
1109	40	0.5174629621736386
1109	41	0.45768943623535563
1109	42	0.4047291144045104
1109	43	0.4740947172834069
1109	44	0.4598175702476013
1109	45	0.4556575211601839
1109	46	0.5926998003243256
1110	1	0.26488498996998544
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2216	5	-1.5701025541289084
2216	6	-2.0902597220253973
2216	7	-2.01591232731111
2216	8	-2.0226066478214086
2216	9	-2.3364602529856597
2216	10	-2.275724505883076
2216	11	-2.0650533073664405
2216	12	-2.4459376477704424
2216	13	-2.320166958207615
2216	14	-4.254434941752407
2216	15	-3.0423499161094227
2216	16	-2.7502199878897358
2216	17	-2.7712043963745145
2216	18	-3.216218794124984
2216	19	-2.7465568250081134
2216	20	-2.566027512225446
2216	21	-2.665746291214056
2216	22	-2.5606513367733257
2216	23	-2.657401298915609
2216	24	-2.58399493413809
2216	25	-2.5906262843209262
2216	26	-2.558956562206724
2216	27	-2.847637767997213
2216	28	-2.6867048326544136
2216	29	-3.0364253242327024
2216	30	-2.932470675951137
2216	31	-2.8416867194375683
2216	32	-2.7816254084473044
2216	33	-2.792748821471335
2216	34	-2.6534839182320837
2216	35	-2.6171130285636437
2216	36	-2.7400174988868784
2216	37	-2.858332608821115
2216	38	-2.909926887724403
2216	39	-2.9538419006511845
2216	40	-2.9218819550300736
2216	41	-3.041896331483507
2216	42	-2.896082228974926
2216	43	-2.8643016989331755
2216	44	-2.9271879536856744
2216	45	-3.0189099965066184
2216	46	-3.312049352396624
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	35519.0
3	1074.0
4	4395.0
5	3298.0
6	2409.0
7	1966.0
8	1744.0
9	1561.0
10	1383.0
11	1266.0
12	1151.0
13	1231.0
14	1254.0
15	1408.0
16	1540.0
17	1691.0
18	1846.0
19	2572.0
20	4581.0
21	7502.0
22	10441.0
23	12343.0
24	11625.0
25	9941.0
26	9784.0
27	11643.0
28	13272.0
29	13369.0
30	13361.0
31	15534.0
32	19546.0
33	24429.0
34	31418.0
35	41893.0
36	59755.0
37	89031.0
38	152060.0
39	480196.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.16295610139467	21.11133701609102	14.663350422082688	22.06235646043163
2	14.015253248166179	23.489492338324585	40.57178285652897	21.923471556980267
3	16.304230050962385	31.62144517560034	29.888979031395994	22.18534574204128
4	10.696415044866566	19.434014829273977	42.285463524064795	27.584106601794662
5	10.973840252403605	41.68145878506187	35.179480737475856	12.165220225058668
6	25.740833751883475	42.559998253008104	19.609074167085218	12.090093828023205
7	25.391162404734345	34.84903078345308	24.461617132167216	15.298189679645361
8	24.7364953886693	35.63863472583146	22.082796497281244	17.542073388217997
9	27.397564402128417	17.104961456991244	20.902394106813997	34.59508003406634
10	13.925163234555498	30.821031598715958	35.035649553425195	20.21815561330334
11	29.219258401939165	28.590250329380762	25.806254958909296	16.38423630977078
12	21.117219516811158	30.54433355898645	30.37127217405863	17.967174750143762
13	25.789603942378385	25.901793578348947	27.686545978643025	20.622056500629647
14	21.405564169196165	25.17833875628735	29.54263388145204	23.873463193064442
15	22.032428596125676	33.037587690963846	24.534999044612064	20.394984668298406
16	21.113670939517686	31.160239192307408	27.791365492542532	19.934724375632374
17	18.390341283388594	32.35659146081811	27.881819207517918	21.371248048275383
18	18.59342343153874	31.307989004779703	30.23840265399359	19.86018490968797
19	20.820780563170878	29.86468940253515	30.633824282751927	18.680705751542046
20	20.438741917696536	30.35643117508003	30.51066145225777	18.69416545496567
21	22.17305653750438	29.445816898012296	29.068202601444032	19.312923963039292
22	20.94161043536494	29.294506438393057	29.812325755755975	19.95155737048603
23	21.006107214738844	30.28667149517574	29.12919416065833	19.578027129427095
24	20.140105875566185	30.237319226296737	29.739605796405183	19.8829691017319
25	20.744223965991644	29.684520083273885	30.21617362314199	19.35508232759248
26	19.633784157579818	30.010991570702732	30.784314082313035	19.570910189404415
27	20.03962692082261	30.687655404150902	29.167197573966003	20.105520101060478
28	20.205272855485543	28.308637201155566	30.949796410454724	20.536293532904164
29	20.032920208076096	29.58924351763979	30.81060325507884	19.567233019205275
30	20.2350459819166	30.27554573669791	29.563274156315767	19.926134125069723
31	21.232720906701996	28.824876070958023	30.625122837156148	19.31728018518384
32	19.775910262196277	30.229822096696708	30.31644440885731	19.677823232249704
33	20.346529112940427	29.772259290836413	29.879901329647602	20.001310266575555
34	20.7174483123841	28.619952939292414	31.047874169931738	19.614724578391744
35	20.14001679692014	30.715980094830712	29.666491054229667	19.477512054019478
36	20.28975086195284	29.692409353872147	29.28355062675895	20.73428915741607
37	21.27900427050603	28.666078823731745	29.810097612866297	20.244819292895926
38	19.533406982606273	30.018418384896922	30.94743662310197	19.500738009394833
39	20.221656579781076	30.4617572301485	29.127247700408574	20.189338489661854
40	20.041078824202835	29.41576884470585	31.234921914742785	19.308230416348536
41	20.324371273458603	29.620319545945765	30.27337757460822	19.781931605987413
42	20.432349361008256	29.52281150904385	29.35228624936383	20.692552880584064
43	20.032091246170623	29.15898363765106	30.10506561139922	20.7038595047791
44	19.447268424765067	30.414252884024666	29.996313909303968	20.1421647819063
45	20.954469953810097	29.371043709754492	28.99560854626954	20.678877790165874
46	20.167059986330134	28.99968783190613	29.539383690991812	21.293868490771928
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3582.0
1	2832.5
2	2083.0
3	2325.5
4	2568.0
5	2385.0
6	2202.0
7	2202.0
8	1972.0
9	1742.0
10	1756.5
11	1771.0
12	2275.5
13	2780.0
14	5227.5
15	7675.0
16	10086.0
17	12497.0
18	11645.5
19	10794.0
20	10794.0
21	10654.0
22	10514.0
23	11989.5
24	13465.0
25	21529.0
26	29593.0
27	33688.0
28	37783.0
29	43550.0
30	49317.0
31	49317.0
32	57932.5
33	66548.0
34	76256.5
35	85965.0
36	87507.5
37	89050.0
38	92760.0
39	96470.0
40	96375.5
41	96281.0
42	90592.5
43	84904.0
44	84904.0
45	77006.0
46	69108.0
47	66138.5
48	63169.0
49	62393.0
50	61617.0
51	56284.5
52	50952.0
53	45578.5
54	40205.0
55	36216.0
56	32227.0
57	32227.0
58	28551.0
59	24875.0
60	21673.5
61	18472.0
62	15149.0
63	11826.0
64	9764.0
65	7702.0
66	6202.0
67	4702.0
68	3852.0
69	3002.0
70	3002.0
71	2339.0
72	1676.0
73	1303.0
74	930.0
75	701.0
76	472.0
77	329.0
78	186.0
79	167.5
80	149.0
81	149.0
82	102.0
83	55.0
84	51.0
85	47.0
86	34.0
87	21.0
88	17.0
89	13.0
90	10.5
91	8.0
92	7.5
93	7.0
94	7.0
95	6.5
96	6.0
97	6.0
98	6.0
99	10.5
100	15.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.20099505746875432
2	0.05850603076161568
3	0.07015264341711615
4	0.06041680315040872
5	0.0431288624899002
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	1.8197832274219493E-4
16	0.0
17	3.6395664548438987E-4
18	8.189024523398773E-4
19	4.549458068554874E-4
20	0.0023657181956485344
21	0.002456707357019632
22	0.0
23	3.6395664548438987E-4
24	5.459349682265849E-4
25	7.279132909687797E-4
26	7.279132909687797E-4
27	0.025840921829391683
28	6.369241295976823E-4
29	0.001000880775082072
30	0.0011828590978242672
31	0.0014558265819375595
32	7.279132909687797E-4
33	0.001728794066050852
34	0.0023657181956485344
35	0.0019107723887930468
36	0.03312005473907948
37	0.00900792697573865
38	0.011828590978242673
39	0.05240975694975215
40	0.015377168271715473
41	0.06014383566629543
42	0.06014383566629543
43	0.02638685679761827
44	0.027933672540926924
45	0.027933672540926924
46	0.02411212776334083
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
46	1099032.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.71781709010263
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.21638867250758	29.509321958903172
2	11.617311892832893	8.995939138907556
3	4.5004554186706756	5.227434291667573
4	2.2395391131363596	3.4684026299417683
5	1.2816857436634643	2.481203709507708
6	0.7970662161358099	1.8516398379027896
7	0.5300871482981586	1.4366672074735545
8	0.3889406522195292	1.2047146425232758
9	0.2865820587711179	0.9986248559524666
>10	1.6321154618603217	12.490854117746059
>50	0.25171623239351476	6.816784700946234
>100	0.23891302170614528	17.9330730240142
>500	0.014221013188540169	3.727331293877051
>1k	0.0047403377295133905	3.1297396109431226
>5k	2.370168864756695E-4	0.7282689796934356
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7936	0.7220899846410296	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3797	0.3454858457260571	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3659	0.33292934145684566	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3223	0.29325806709904717	No Hit
GAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAG	2023	0.1840710734537302	No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGC	1890	0.17196951499137422	No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAAT	1738	0.15813916246296741	No Hit
GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATT	1682	0.15304376942618594	No Hit
TTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCT	1514	0.13775759031584156	No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGC	1491	0.13566483960430634	No Hit
CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAG	1435	0.1305694465675249	No Hit
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGA	1323	0.12037866049396195	No Hit
GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAAT	1274	0.11592019158677819	No Hit
CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCAT	1249	0.11364546255250076	No Hit
ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCC	1233	0.11218963597056318	No Hit
TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACC	1141	0.10381863312442222	No Hit
ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGA	1134	0.10318170899482454	No Hit
GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAG	1131	0.10290874151071125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	9.098916137109748E-5	0.0
17	0.0	0.0	0.0	9.098916137109748E-5	0.0
18	0.0	0.0	0.0	9.098916137109748E-5	0.0
19	0.0	0.0	0.0	9.098916137109748E-5	0.0
20	0.0	0.0	0.0	1.8197832274219496E-4	0.0
21	0.0	0.0	0.0	2.729674841132924E-4	0.0
22	0.0	0.0	0.0	7.279132909687798E-4	0.0
23	0.0	0.0	0.0	0.0011828590978242672	0.0
24	0.0	0.0	0.0	0.0012738482591953646	0.0
25	0.0	0.0	0.0	0.0014558265819375597	0.0
26	0.0	0.0	0.0	0.0026386856797618267	0.0
27	0.0	0.0	0.0	0.003093631486617314	0.0
28	0.0	0.0	0.0	0.004003523100328289	0.0
29	0.0	0.0	0.0	0.005823306327750239	0.0
30	0.0	0.0	0.0	0.009189905298480844	0.0
31	0.0	0.0	0.0	0.015013211626231083	0.0
32	0.0	0.0	0.0	0.021382452922207907	0.0
33	0.0	0.0	0.0	0.027296748411329244	0.0
34	0.0	0.0	0.0	0.03484884880513033	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTAGCG	25	0.0037613988	31.98617	28
CGCGGCA	35	5.6061527E-4	28.586391	37
ACGCGGC	35	5.6061527E-4	28.586391	36
GGTATCA	1600	0.0	27.44057	1
CCGGTCC	45	7.812492E-5	26.655142	9
CAACGCG	40	0.0012237562	25.009676	34
CGCGTTA	65	1.35558E-6	24.628277	39
AGTTACG	60	2.0322737E-5	23.345552	38
GTATCAA	4255	0.0	23.039778	1
TCGCGTT	70	2.5825902E-6	22.869114	38
CTCGGGT	45	0.0024272583	22.232847	35
CCAACGC	45	0.0024304702	22.227787	33
CCCAACG	45	0.0024343291	22.221716	32
GCGGCAA	55	3.0275996E-4	21.829607	38
ATGCGTA	265	0.0	21.122944	13
GGTCGGT	225	0.0	20.435608	11
AACGCGG	50	0.00447097	20.009563	35
TAGTCGG	240	0.0	20.00683	40
CGACTAA	110	1.5697879E-8	20.005007	33
CCGACTA	110	1.5746991E-8	19.999544	32
>>END_MODULE