FastQCFastQC Report
Mon 6 Feb 2017
SRR2077311_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077311_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1099032
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33840.3079073220797938No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22590.20554451553730918No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19890.18097744196711288No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATGACGAGACGTAATTCGTGAAGGAACC18030.16405345795208875No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACTGCAAATTCTAGAATTGTGAGTAGAA17010.15477256349223678No Hit
GAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAA16960.15431761768538133No Hit
GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTCTGAGCGTTCTATCATTCTAGCCTCG13670.12438218359429026No Hit
TTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTC13050.1187408555892822No Hit
CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGTACTGAAGTATTAAGTAGTGGGACTT13020.11846788810516891No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGTGAGCTTTTTAGTTTGTGTCGGAAGC12610.11473733248895393No Hit
ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTAGAAGTCCCACTACTTAATACTTCAG12310.112007657647821No Hit
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTCATCATAGCCTTATAGAAGGTAAACG12190.11091578771136783No Hit
CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCACTATGAGCTGGAGCCGTAATTACAG11890.10818611287023491No Hit
TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATGGCATTAGCAGTCCGGCTTACAGCTA11450.1041825897699066No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACATCGC200.007373835451.72355328
CCGAACG200.00737914451.71413867
GGTATCA25700.041.4512941
GTATCAA63850.040.3794861
ATGGGTA3250.032.0148935
GTGCGAA450.004622841330.66634223
AACGCGT450.004634178230.65099328
GTCGGAA4150.029.07622167
TATCAAC90250.028.3995462
TGTGTCG4300.028.06193564
ATCAACG92000.027.8970343
GAACGCG500.00773517127.59970927
TCAACGC93350.027.4192894
CAACGCA95100.026.9147285
CATGGGT5600.026.6314354
AACGCAG97300.026.3394096
GTGTCGG4750.025.40343565
GACATAT2500.024.9841961
GTTATCG700.00133920824.6257849
TGCGGTA851.4491528E-424.3382851
Produced by FastQC (version 0.11.5)