##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2077311_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1099032 Sequences flagged as poor quality 0 Sequence length 75 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.1422342570553 33.0 31.0 34.0 28.0 34.0 2 31.179980200758486 33.0 31.0 34.0 28.0 34.0 3 31.29625252039977 33.0 31.0 34.0 28.0 34.0 4 34.713311350351944 37.0 35.0 37.0 32.0 37.0 5 34.48926509874144 37.0 35.0 37.0 31.0 37.0 6 34.29473391129649 37.0 35.0 37.0 30.0 37.0 7 34.23803037582163 37.0 35.0 37.0 30.0 37.0 8 34.19322276330443 37.0 35.0 37.0 30.0 37.0 9 35.70706312464059 39.0 37.0 39.0 30.0 39.0 10 35.66189610493598 39.0 37.0 39.0 30.0 39.0 11 35.71572165323666 39.0 37.0 39.0 30.0 39.0 12 35.664645797392616 39.0 37.0 39.0 30.0 39.0 13 35.623831699167994 39.0 37.0 39.0 30.0 39.0 14 36.81422015009572 40.0 37.0 41.0 30.0 41.0 15 36.81599170906762 40.0 37.0 41.0 30.0 41.0 16 36.865558054724524 40.0 37.0 41.0 30.0 41.0 17 36.91616986584558 40.0 37.0 41.0 30.0 41.0 18 36.97164413775031 40.0 37.0 41.0 30.0 41.0 19 37.03816631362872 40.0 38.0 41.0 30.0 41.0 20 37.056926458920216 40.0 38.0 41.0 30.0 41.0 21 37.07605602020687 40.0 38.0 41.0 30.0 41.0 22 37.01968914462909 40.0 37.0 41.0 30.0 41.0 23 37.04109434484164 40.0 37.0 41.0 30.0 41.0 24 37.0245142998566 40.0 37.0 41.0 30.0 41.0 25 36.98265018670976 40.0 37.0 41.0 30.0 41.0 26 36.93310203888513 40.0 37.0 41.0 30.0 41.0 27 36.89703302542601 40.0 37.0 41.0 30.0 41.0 28 36.838190334767326 40.0 37.0 41.0 30.0 41.0 29 36.79688125550484 40.0 37.0 41.0 30.0 41.0 30 36.67398765459058 40.0 37.0 41.0 30.0 41.0 31 36.523730883177194 40.0 36.0 41.0 30.0 41.0 32 36.37707364298765 40.0 35.0 41.0 30.0 41.0 33 36.12685708878358 40.0 35.0 41.0 29.0 41.0 34 36.13560660654103 40.0 35.0 41.0 29.0 41.0 35 36.05101671288916 40.0 35.0 41.0 29.0 41.0 36 35.97540108022332 40.0 35.0 41.0 28.0 41.0 37 35.902033789734965 40.0 35.0 41.0 28.0 41.0 38 35.80560711608033 40.0 35.0 41.0 27.0 41.0 39 35.734247956383435 40.0 35.0 41.0 27.0 41.0 40 35.69593696998813 40.0 35.0 41.0 27.0 41.0 41 35.57346373899941 40.0 35.0 41.0 26.0 41.0 42 35.483391748374935 40.0 35.0 41.0 25.0 41.0 43 35.3892743796359 40.0 35.0 41.0 25.0 41.0 44 35.221767883009775 40.0 35.0 41.0 24.0 41.0 45 35.073180762707544 40.0 35.0 41.0 24.0 41.0 46 34.861491749102846 40.0 35.0 41.0 22.0 41.0 47 34.5978379155475 40.0 35.0 41.0 17.0 41.0 48 34.36339251268389 40.0 35.0 41.0 13.0 41.0 49 34.22338021094927 40.0 34.0 41.0 9.0 41.0 50 34.09227483822127 40.0 34.0 41.0 7.0 41.0 51 34.00337842756171 40.0 34.0 41.0 5.0 41.0 52 33.89700481878599 39.0 34.0 41.0 2.0 41.0 53 33.822220827055084 39.0 34.0 41.0 2.0 41.0 54 33.72511173469017 39.0 34.0 41.0 2.0 41.0 55 33.6985092335801 39.0 34.0 41.0 2.0 41.0 56 33.572770401589764 39.0 34.0 41.0 2.0 41.0 57 33.520211422415365 39.0 34.0 41.0 2.0 41.0 58 33.39539522052133 39.0 33.0 41.0 2.0 41.0 59 33.30175736466272 39.0 33.0 41.0 2.0 41.0 60 33.20795572831364 39.0 33.0 41.0 2.0 41.0 61 33.131246405928124 39.0 33.0 41.0 2.0 41.0 62 33.00732735716522 38.0 33.0 41.0 2.0 41.0 63 32.87085362391632 38.0 33.0 41.0 2.0 41.0 64 32.76541538371949 38.0 33.0 40.0 2.0 41.0 65 32.57010896861966 38.0 33.0 40.0 2.0 41.0 66 32.421617386936866 37.0 33.0 40.0 2.0 41.0 67 32.26351462013845 37.0 32.0 40.0 2.0 41.0 68 32.09780788912425 37.0 32.0 40.0 2.0 41.0 69 32.023314152818116 37.0 32.0 39.0 2.0 41.0 70 31.94253215556963 37.0 32.0 39.0 2.0 41.0 71 31.79524436049178 36.0 32.0 39.0 2.0 41.0 72 31.611486289753163 36.0 32.0 39.0 2.0 41.0 73 31.417653899067542 36.0 32.0 39.0 2.0 41.0 74 31.18196922382606 36.0 32.0 39.0 2.0 41.0 75 30.97387519198713 36.0 32.0 39.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -8.463615777003497E-4 1101 2 0.14417204349284418 1101 3 -0.04152285389447741 1101 4 0.09148021717601296 1101 5 0.06440006599410708 1101 6 -0.04251656408632698 1101 7 -0.018674684262400376 1101 8 0.11442639397994014 1101 9 -0.035462397368206666 1101 10 0.08754593825918278 1101 11 0.11249536143619565 1101 12 0.11107182521692494 1101 13 0.03808240012838837 1101 14 0.03370637636522389 1101 15 -0.3991266522331429 1101 16 0.033396733907835596 1101 17 -0.10793676979987765 1101 18 0.007034386089955547 1101 19 -0.030865016605211792 1101 20 0.00623512801354309 1101 21 0.03421007640148588 1101 22 0.025872294829838438 1101 23 -0.05074705692219794 1101 24 0.04537872536529619 1101 25 -0.10486399398569546 1101 26 -0.08583545532286507 1101 27 -0.09829154324032174 1101 28 -0.11361098100847755 1101 29 -0.24514424544666724 1101 30 -0.2124225056261153 1101 31 -0.0949812715143139 1101 32 -0.23206313215900565 1101 33 0.15016373474141886 1101 34 -0.05627705848855413 1101 35 -0.11647753391944349 1101 36 -0.21004871521503077 1101 37 -0.19048465474622134 1101 38 -0.16884156787669014 1101 39 -0.02600937765572553 1101 40 0.08324891498984499 1101 41 -0.11784895358658787 1101 42 -0.06070944225228203 1101 43 0.07105689874514098 1101 44 -0.05016076828960081 1101 45 -0.11003000756178949 1101 46 -0.051186613854177665 1101 47 0.01703754967719817 1101 48 0.04778002135950743 1101 49 0.07702907689483851 1101 50 0.15532254896771747 1101 51 0.09138185233410212 1101 52 -0.5794034054318473 1101 53 -0.18152065392808936 1101 54 0.04435382229750218 1101 55 -0.12710459594462264 1101 56 -0.024285367610268338 1101 57 -0.21717563580773458 1101 58 0.17895125534266754 1101 59 0.0879836480356957 1101 60 0.040043910778052805 1101 61 -0.0844022774549984 1101 62 -0.16888365898184787 1101 63 -0.1726532685586406 1101 64 -0.025917448445724744 1101 65 -0.14800810829005684 1101 66 -0.03233147461391184 1101 67 0.06715599562490837 1101 68 0.05477333076503754 1101 69 0.04846044866665977 1101 70 0.08707158926149106 1101 71 0.06416858277817283 1101 72 0.04708406099896578 1101 73 0.002354022200300676 1101 74 -0.003748756882401949 1101 75 -0.006921453887393625 1102 1 0.5500856013128548 1102 2 0.4139702056630874 1102 3 0.4471376278650574 1102 4 0.5327570192003108 1102 5 0.5096852007765946 1102 6 0.41752609617245184 1102 7 0.5521841852611615 1102 8 0.3801112675571616 1102 9 0.5839799971930404 1102 10 0.5948763069645366 1102 11 0.57418913624835 1102 12 0.5028846166786636 1102 13 0.6887734546649895 1102 14 0.7249083412037933 1102 15 0.8976839563922638 1102 16 0.853991273999128 1102 17 0.7875786756559648 1102 18 0.86760035532442 1102 19 0.8160342327359515 1102 20 0.7997065483763635 1102 21 0.7540935713409382 1102 22 0.808799941871122 1102 23 0.8592004284054227 1102 24 0.8829618171028173 1102 25 0.8702880184097097 1102 26 0.9929749417573177 1102 27 0.7744288834112751 1102 28 0.8256726811252122 1102 29 0.9565522819037184 1102 30 0.9345144379701296 1102 31 0.9273469592774362 1102 32 0.8692376259324988 1102 33 1.125866254784107 1102 34 0.9277843566019754 1102 35 0.9683990653808223 1102 36 0.8985887416029925 1102 37 0.8602486271573895 1102 38 0.9187393798214032 1102 39 1.0716881690322637 1102 40 1.053909062687616 1102 41 0.8911478442394838 1102 42 0.9434075218368889 1102 43 0.9087592399136852 1102 44 0.8098334570975396 1102 45 0.7621970858272604 1102 46 0.9572351730168691 1102 47 1.0328948652826568 1102 48 1.0290648032943608 1102 49 1.0294701985092445 1102 50 1.0064576034406159 1102 51 1.0874633650376353 1102 52 1.060697727886975 1102 53 1.023411126821216 1102 54 1.060088212975792 1102 55 1.019785791366914 1102 56 -2.0584666651339205 1102 57 -0.067616021506133 1102 58 0.7003497897998301 1102 59 0.9732910154860761 1102 60 0.9847309606573944 1102 61 1.0413535985402902 1102 62 0.8339762854201496 1102 63 0.9306494097754126 1102 64 0.8459343359464029 1102 65 0.9312927633334382 1102 66 0.9717029950058702 1102 67 0.8754245551448747 1102 68 0.280227856913946 1102 69 0.6942185789662645 1102 70 0.8831758900412296 1102 71 1.0191625406708695 1102 72 1.011399485545315 1102 73 1.0707995201054707 1102 74 1.0139536577960584 1102 75 1.046828185199299 1103 1 0.49749884850046655 1103 2 0.5552510801555215 1103 3 0.5254972539844331 1103 4 0.4248780781850243 1103 5 0.424525777269686 1103 6 0.4406037670421057 1103 7 0.4516387294992015 1103 8 0.47970742012419976 1103 9 0.5908541802786758 1103 10 0.5789022734768068 1103 11 0.6248288729308484 1103 12 0.6906821348726595 1103 13 0.6292460045713 1103 14 0.8009639785573839 1103 15 0.8861644061384268 1103 16 0.6554995325827093 1103 17 0.7536237691320196 1103 18 0.7258101946237616 1103 19 0.8474694713270949 1103 20 0.7135250102769106 1103 21 0.8613664066679263 1103 22 0.8779436129273321 1103 23 0.9016526218192595 1103 24 0.8490494166920826 1103 25 0.8891682106003174 1103 26 0.8700917363555831 1103 27 0.828898106033499 1103 28 0.9121116173546326 1103 29 0.9961101238683483 1103 30 0.9743088185832463 1103 31 0.99056456971757 1103 32 1.0200225524776059 1103 33 1.1516320437388927 1103 34 0.9778467185800963 1103 35 0.9549785472864016 1103 36 0.8935348434578714 1103 37 0.9241140622487833 1103 38 0.9096662925276249 1103 39 1.005755283823568 1103 40 0.9695056969905949 1103 41 0.8898793516203938 1103 42 0.9110623375630524 1103 43 0.9904638609972665 1103 44 1.0139781179951868 1103 45 1.007519937467933 1103 46 1.0113025554392507 1103 47 1.110578329341969 1103 48 1.1763130637907153 1103 49 1.1495598076930875 1103 50 1.0867941573991757 1103 51 1.1296983049839753 1103 52 1.1181549728375018 1103 53 1.1373625383980652 1103 54 1.0342708656799857 1103 55 1.108820355527726 1103 56 1.0862757025589147 1103 57 1.086786647562917 1103 58 1.1335209308252772 1103 59 1.2146474170444748 1103 60 1.0970879994139153 1103 61 1.1025624015563409 1103 62 0.9741429487414308 1103 63 0.9553509715995716 1103 64 1.1069990117526771 1103 65 1.0611398869486948 1103 66 1.1597394132990857 1103 67 1.1688816442396543 1103 68 1.0963141380265498 1103 69 1.1029262711165302 1103 70 1.1679888887255032 1103 71 1.2745624897422516 1103 72 1.1141733636495879 1103 73 1.190050420703745 1103 74 1.1819075119001212 1103 75 1.262174781227106 1104 1 0.4937183046846698 1104 2 0.5589512772753231 1104 3 0.5533087992638528 1104 4 0.4435073094718689 1104 5 0.5272826346477331 1104 6 0.503816143832573 1104 7 0.5001047835995678 1104 8 0.5794705905121162 1104 9 0.6587023296586381 1104 10 0.5203689328813681 1104 11 0.6031692578456003 1104 12 0.5645675658921263 1104 13 0.7026380759747894 1104 14 0.4962726972168596 1104 15 0.6683700079255246 1104 16 0.7544453298517695 1104 17 0.746268183626718 1104 18 0.7124629860340832 1104 19 0.7133750848185443 1104 20 0.6901248076332749 1104 21 0.7062787418558045 1104 22 0.9265776997813404 1104 23 0.7657125789960162 1104 24 0.7454214929704577 1104 25 0.8477854130596967 1104 26 0.8092376123489515 1104 27 0.8876400390127657 1104 28 0.8248373365251567 1104 29 0.779751150281939 1104 30 0.7627378127700908 1104 31 0.7557934554363683 1104 32 0.9021686281788703 1104 33 1.0175268413289587 1104 34 0.8383150239330845 1104 35 0.8149844728552296 1104 36 0.8619960613051916 1104 37 0.9722699317487553 1104 38 1.0252720544673153 1104 39 0.7985071620241584 1104 40 0.6765879105884238 1104 41 0.6687358868004623 1104 42 0.6591827849859868 1104 43 0.6480656434578123 1104 44 0.8402731966441692 1104 45 0.7639350224055974 1104 46 0.7962476705339654 1104 47 0.8374949746665408 1104 48 0.8964829043450138 1104 49 0.7805942598155298 1104 50 0.6966209446013352 1104 51 0.8395331654839566 1104 52 0.776956161505062 1104 53 0.8162616032475114 1104 54 0.736526869567534 1104 55 0.7357208156284472 1104 56 0.9059120487774592 1104 57 0.9402095520109981 1104 58 0.9862805887492172 1104 59 0.8168899396835059 1104 60 0.697502248436912 1104 61 0.5340148882672651 1104 62 0.6550877640811592 1104 63 0.7277423017275382 1104 64 0.7769579615440989 1104 65 0.7730942778376857 1104 66 0.7555267742230072 1104 67 0.824599142166285 1104 68 0.8903364983575344 1104 69 0.9175482881971533 1104 70 0.9389189133200944 1104 71 0.8253105058934835 1104 72 0.9131946018308064 1104 73 0.8237621947008513 1104 74 0.8278354602279236 1104 75 0.8936580932569775 1105 1 0.25377151891947847 1105 2 0.2983204985420116 1105 3 0.28668799819956803 1105 4 0.253991273932364 1105 5 0.0010010743303467962 1105 6 0.17164566823034733 1105 7 0.17435042430845726 1105 8 0.2972283707183223 1105 9 0.12131571874021319 1105 10 0.2593975830452848 1105 11 0.310252262549362 1105 12 0.20186162205214941 1105 13 0.2786418769915642 1105 14 0.28712783096513306 1105 15 0.3581694092653862 1105 16 0.3459310522825234 1105 17 0.38618912247786596 1105 18 0.2956613515968698 1105 19 0.4791442680750677 1105 20 0.31497091396671806 1105 21 0.4737891708955715 1105 22 0.30764217452833265 1105 23 0.2500753860469018 1105 24 0.2791382221955203 1105 25 0.26738459584109364 1105 26 0.2741042441729675 1105 27 0.08836527301286168 1105 28 0.23648935343967992 1105 29 0.2513896735869281 1105 30 0.28225128262327104 1105 31 0.35153517634671516 1105 32 0.3865166112643479 1105 33 0.43412360096215963 1105 34 0.295525267673284 1105 35 0.6141505297754577 1105 36 0.4846242743521785 1105 37 0.5154086213482216 1105 38 0.39749118308422027 1105 39 0.42717367031512055 1105 40 0.5015031954271194 1105 41 0.5440081821394642 1105 42 0.6344894795267777 1105 43 0.6767127665632415 1105 44 0.48179550386134906 1105 45 0.6308595247099618 1105 46 0.5303019300483882 1105 47 0.6369423068278977 1105 48 0.6368308874304915 1105 49 0.5176448608513056 1105 50 0.5919648891064924 1105 51 0.5414456470727842 1105 52 0.6493419647074177 1105 53 0.48270337642183136 1105 54 0.5851251496074426 1105 55 0.5364126006809471 1105 56 0.4824533135657916 1105 57 0.48598614725022315 1105 58 0.4530306243837501 1105 59 0.6754103939050111 1105 60 0.5376983809023486 1105 61 0.3689488931135614 1105 62 0.4820103658771373 1105 63 0.4767368377659267 1105 64 0.5304836896763732 1105 65 0.5883093678742739 1105 66 0.6113416647062095 1105 67 0.595828486015769 1105 68 0.5684242068195431 1105 69 0.5465785537932035 1105 70 0.5576974565804136 1105 71 0.5533067048767109 1105 72 0.6101733636495901 1105 73 0.711326218077101 1105 74 0.4762364674973725 1105 75 0.4416719669681939 1106 1 -0.22065179454442685 1106 2 0.023724226425446915 1106 3 -0.14480637212874825 1106 4 -0.14574493573742586 1106 5 0.032053775626593506 1106 6 -0.06787708699720696 1106 7 -0.0712826535607789 1106 8 -0.11451313746599112 1106 9 0.15524059337357698 1106 10 -0.1077111311187764 1106 11 0.12695658651567499 1106 12 0.12383569717751186 1106 13 0.3076035573775968 1106 14 0.1370042620371379 1106 15 0.06284194214455852 1106 16 -0.09701530025792948 1106 17 0.08278936848032004 1106 18 0.054394249695086216 1106 19 0.052614984320690894 1106 20 0.2046414599029447 1106 21 0.03986126853962446 1106 22 -0.06833738834421865 1106 23 0.11889287114482983 1106 24 0.00218940963047487 1106 25 -0.008924231862572185 1106 26 -0.08397088119367169 1106 27 -0.14349469481758348 1106 28 -0.31030599143684157 1106 29 -0.2671032774964317 1106 30 -0.24541453249641165 1106 31 -0.20437853355961977 1106 32 -0.1810152271309633 1106 33 0.17053915588693513 1106 34 0.040013109474777764 1106 35 0.06759967349766782 1106 36 -0.020599682502769667 1106 37 -0.01684419348762134 1106 38 -0.16784063638782243 1106 39 -0.1418288843258111 1106 40 -0.08351752554931835 1106 41 -0.01820319074349186 1106 42 -0.032277480450510154 1106 43 -0.05213310343391697 1106 44 -0.13734916450840728 1106 45 -0.19001473018643367 1106 46 -0.3143645473856225 1106 47 -0.2894306707480325 1106 48 -0.02495433349864129 1106 49 -0.04741408512276735 1106 50 -0.01673110862271443 1106 51 -0.14404058625109428 1106 52 -0.16468093244462523 1106 53 -0.214306747525562 1106 54 -0.20598999846711763 1106 55 -0.3013979774248341 1106 56 -0.221788147310356 1106 57 -0.20347548582764574 1106 58 -0.17912719565598678 1106 59 -0.16100364715847348 1106 60 -0.23338838227297742 1106 61 -0.14737355934173024 1106 62 -0.21635087927377583 1106 63 -0.06901734900671386 1106 64 -0.11855708996218794 1106 65 -0.1485875949415103 1106 66 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-2.3307491694309235 2216 28 -2.3432150140491146 2216 29 -2.366869528289044 2216 30 -2.2075355772234673 2216 31 -2.47596986950564 2216 32 -2.4986412966959577 2216 33 -2.6239717886781406 2216 34 -2.5096500599930636 2216 35 -2.7438455340409647 2216 36 -2.619763649101607 2216 37 -2.756737436187059 2216 38 -2.599058710136333 2216 39 -2.6572077207489 2216 40 -2.60119836648402 2216 41 -2.812555110854966 2216 42 -2.680465505607849 2216 43 -2.6966772924795066 2216 44 -2.477157638804364 2216 45 -2.6185722411289873 2216 46 -2.7939608730168146 2216 47 -2.6738262539526403 2216 48 -2.8396563618471795 2216 49 -2.7194805526573376 2216 50 -2.5783207773228867 2216 51 -2.754869247613371 2216 52 -2.8223297408325507 2216 53 -2.878077160406608 2216 54 -2.688628210804602 2216 55 -2.653222674223791 2216 56 -2.653900775458954 2216 57 -2.5601207996950492 2216 58 -2.8618231924580897 2216 59 -2.9402438919289153 2216 60 -2.990804780606801 2216 61 -2.911151940184876 2216 62 -2.8688342386201597 2216 63 -3.119353108216238 2216 64 -2.9421048953912496 2216 65 -2.9202512451653817 2216 66 -2.9016656632951268 2216 67 -3.1304003988717994 2216 68 -2.8983977855593324 2216 69 -2.731701250235105 2216 70 -2.87778413966765 2216 71 -2.6208287165880506 2216 72 -2.805302370197399 2216 73 -2.6476989483732147 2216 74 -2.7206986692221946 2216 75 -2.6033556281911387 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 18545.0 3 6535.0 4 3671.0 5 2359.0 6 1845.0 7 1689.0 8 1879.0 9 2067.0 10 2327.0 11 2583.0 12 2721.0 13 2968.0 14 3069.0 15 3567.0 16 4289.0 17 5154.0 18 5545.0 19 6187.0 20 7402.0 21 8882.0 22 12269.0 23 15798.0 24 14931.0 25 13299.0 26 12216.0 27 10649.0 28 11385.0 29 14245.0 30 16021.0 31 16704.0 32 19519.0 33 24500.0 34 31947.0 35 44427.0 36 60246.0 37 87070.0 38 158946.0 39 340853.0 40 100723.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.24889654469176 21.209467136697356 16.033424134716235 22.508212183894653 2 13.335741695019514 23.18351443060597 40.58577786594282 22.89496600843169 3 15.238639444888275 31.14496343112331 31.073718269515986 22.54267885447243 4 10.107245632907592 19.325419144401184 41.67062558132339 28.896709641367835 5 10.349134110603924 41.12717292190644 35.44585264895383 13.077840318535799 6 25.377765908604523 42.87809972910238 19.140352770527215 12.603781591765884 7 24.274677267683256 35.0133954052349 24.7713495813879 15.940577745693943 8 25.284705085930163 34.212652588823616 21.98853172610079 18.514110599145432 9 27.17227523857358 15.592266649196748 21.501921691088157 35.733536421141515 10 13.967938825721731 30.180414622428625 34.99031868275852 20.86132786909112 11 29.199058808114774 27.56916996047431 26.090141142387118 17.141630089023796 12 21.65405556890063 29.76228171700187 31.12775606169793 17.455906652399566 13 26.484976622818714 24.760243956382013 28.68471613812788 20.070063282671395 14 21.707916287534122 23.547042766151048 31.27761601455869 23.467424931756142 15 21.88349947450556 31.36178131241668 26.74935052526696 20.005368687810805 16 20.346211626952602 29.85929693052018 29.399507361819722 20.39498408070749 17 17.73165425007712 30.53552855360609 29.861602117690524 21.871215078626268 18 17.82915335543203 28.68975595286299 33.641034786794286 19.8400559049107 19 19.894867296577225 27.613485812453593 34.42299852956163 18.068648361407543 20 19.313644486989563 27.84022042578283 34.57438096243371 18.271754124793897 21 20.97063772206889 27.032550842776637 33.28983635855703 18.706975076597445 22 19.668188002529998 27.115119469610445 33.92764023061206 19.289052297247487 23 20.010520261514046 27.67666030840019 33.22009187937737 19.09272755070839 24 19.20300438518664 27.898850424095507 33.759658284854225 19.138486905863626 25 19.773908992136043 27.526848648422387 34.02412147000104 18.67512088944053 26 18.759543478221303 27.62280716122586 34.85419294251255 18.763456418040292 27 19.10572621057854 28.392737666640876 33.23159269761366 19.269943425166925 28 19.100466120281236 26.14496140538632 34.922164647297464 19.832407827034977 29 19.108702220054383 27.145251762684598 34.91653410890242 18.8295119083586 30 19.28252916659083 27.950603352565384 33.5368472780882 19.230020202755583 31 20.158130460814984 26.648777123223105 34.55493618546889 18.638156230493028 32 18.885235936918484 27.943899635928183 34.224024736136656 18.94683969101668 33 19.486332532576252 27.494449297517654 33.72297444856956 19.296243721336538 34 19.722161303326658 26.518526068080128 34.87379778708194 18.885514841511277 35 19.288795947208882 28.35352175863616 33.602512337371024 18.755169956783938 36 19.520280261152436 27.342933187743125 33.0607156668714 20.076070884233037 37 20.20440902551746 26.627951733118106 33.51711097542888 19.65052826593556 38 18.618992156363174 27.87384665780997 34.65222297039073 18.854938215436132 39 19.349200239850703 28.26134627580829 32.72897507499884 19.660478409342165 40 19.22526778597504 27.095882483539764 34.843295614606575 18.835554115878626 41 19.422585552991535 27.501801690313094 33.5973385940264 19.478274162668978 42 19.669547525064196 27.415107490777796 32.70875304146216 20.20659194269585 43 19.30437251303255 27.094694257124115 33.31546826645459 20.285464963388744 44 18.785863126381976 28.236712558122605 33.12046734669736 19.856956968798055 45 20.154413805340337 27.27555630371385 32.178945309123336 20.391084581822483 46 19.71918643956758 27.066816739625537 32.254160748520725 20.95983607228615 47 20.038889970682387 27.99914103574347 32.14646432477584 19.815504668798305 48 19.575161168511517 28.430079935942054 31.32005768854555 20.67470120700088 49 20.336709772991153 27.719228963373578 31.773595344080192 20.17046591955508 50 20.085184672946177 27.564366201284045 31.407321405174045 20.94312772059573 51 20.52224647697007 28.45994888000997 30.0920032429983 20.92580140002166 52 20.18982297019901 27.950345033411462 31.0421792426735 20.817652753716022 53 20.26407066633668 28.648418960823424 30.666482260623972 20.42102811221593 54 20.182057905842722 28.883494041352474 30.69915950176116 20.235288551043638 55 21.02792513261936 27.53860292445019 30.655863003976307 20.777608938954153 56 20.09010550224126 29.170268642156856 30.14002660760337 20.599599247998515 57 20.72673799613809 28.018141150820153 29.95528410155987 21.299836751481884 58 19.891666924472577 27.435037460714927 31.127697635525532 21.545597979286963 59 19.884220650471754 28.91635660840392 30.733302577737497 20.46612016338683 60 20.19723079806773 29.456232251184467 29.749585311237226 20.59695163951058 61 21.285329125822233 28.522266414434384 30.129109807486348 20.06329465225703 62 20.03628882189632 29.097938871352124 30.907830214175025 19.957942092576534 63 20.18019559101315 28.39782061967484 31.059536244754455 20.362447544557554 64 20.75353583881088 27.66625539565727 30.58900923721966 20.991199528312187 65 19.881131759584797 28.448216248480477 30.920573741255943 20.75007825067878 66 20.24863698236266 28.707626347549482 29.944078061421326 21.099658608666534 67 20.356732106071522 28.41928169516447 30.146073999665155 21.077912199098844 68 20.136447346392096 28.39835418804912 31.108284381164513 20.35691408439427 69 20.450723909767866 29.11007140829384 29.279493226766828 21.15971145517146 70 20.788232382422095 27.66520232644475 30.712655684721607 20.833909606411556 71 20.769367952889453 28.332205067732332 30.406121022863758 20.49230595651446 72 20.451451823058836 29.265844852561163 29.14846883439245 21.134234489987552 73 20.395493488815614 27.707382496597006 30.352346428493437 21.544777586093943 74 19.949191421138877 29.02057911225111 29.441314908550925 21.588914558059084 75 20.57774477904192 28.521280545061472 29.76128083622679 21.139693839669818 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1740.0 1 1122.5 2 769.5 3 1043.0 4 1052.0 5 774.0 6 601.0 7 1051.5 8 1397.0 9 2681.0 10 4657.5 11 4934.0 12 4518.0 13 3855.0 14 3206.5 15 3355.5 16 3490.0 17 3700.5 18 4236.5 19 5043.5 20 5525.0 21 5972.0 22 6811.0 23 7770.0 24 8337.0 25 9361.5 26 11446.5 27 15394.5 28 18282.0 29 20790.5 30 26331.0 31 32860.0 32 36357.0 33 45064.0 34 59107.5 35 67827.5 36 71211.0 37 71388.0 38 68326.5 39 62647.5 40 60207.0 41 60186.0 42 55893.0 43 50291.5 44 48962.0 45 46965.0 46 41401.5 47 39588.5 48 41342.0 49 38832.5 50 33429.0 51 29075.0 52 27615.0 53 26252.5 54 23321.0 55 20245.5 56 18739.0 57 17575.5 58 15046.5 59 12558.5 60 11436.0 61 10085.5 62 7557.0 63 5488.0 64 4597.0 65 3891.5 66 2652.5 67 1806.0 68 1493.0 69 1327.0 70 948.0 71 588.0 72 441.0 73 356.5 74 239.5 75 171.0 76 135.0 77 108.0 78 66.5 79 38.5 80 25.0 81 19.0 82 12.5 83 8.0 84 4.0 85 2.5 86 2.0 87 1.5 88 0.0 89 0.0 90 0.0 91 0.5 92 1.0 93 1.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06041680315040872 2 0.00755210039380109 3 9.098916137109747E-4 4 0.022383333697289978 5 0.11455535416621172 6 0.5122689785192788 7 0.04740535307434179 8 0.0 9 0.0 10 7.279132909687797E-4 11 0.0 12 0.0 13 0.028297629186411318 14 0.002911653163875119 15 0.0060962738118635305 16 0.004640447229925971 17 0.00900792697573865 18 0.0020927507115352417 19 0.003639566454843899 20 0.006187262973234629 21 0.009553861943965234 22 0.019744648017528153 23 0.018197832274219495 24 0.009553861943965234 25 0.008552981168883162 26 0.010372764396305112 27 0.04431172158772447 28 0.015832114078570964 29 0.012738482591953645 30 0.015650135755828767 31 0.006005284650492433 32 0.006642208780090116 33 0.006551219618719018 34 0.011009688525902795 35 0.01228353678509816 36 0.005186382198152556 37 0.005368360520894751 38 0.004731436391297069 39 0.0013648374205664621 40 0.0018197832274219495 41 0.005823306327750238 42 0.004913414714039264 43 0.004822425552668166 44 0.005641328005008044 45 0.0033665989707306063 46 0.001000880775082072 47 0.0030936314866173145 48 0.002456707357019632 49 0.004003523100328288 50 7.279132909687797E-4 51 0.004458468907183776 52 7.279132909687797E-4 53 7.279132909687797E-4 54 0.0037305556162149965 55 0.002001761550164144 56 0.008916937814367552 57 0.008552981168883162 58 0.0012738482591953646 59 0.004094512261699387 60 0.0013648374205664621 61 0.004094512261699387 62 0.006460230457347921 63 3.6395664548438987E-4 64 0.0 65 0.0 66 0.0 67 0.0 68 0.0 69 0.0 70 0.0014558265819375595 71 0.0 72 0.0 73 0.0 74 4.549458068554874E-4 75 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 75 1099032.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.124434336370975 #Duplication Level Percentage of deduplicated Percentage of total 1 80.8673006589269 36.49091198543316 2 9.532590867181213 8.603055412832166 3 3.6077799451638954 4.8839708770687285 4 1.7741030691901711 3.202215898065044 5 1.0374583114996505 2.3407359726994144 6 0.5881580829163433 1.5924180475178622 7 0.4179714533573282 1.320250778105021 8 0.30148733257305027 1.0883556273552197 9 0.21334442565446593 0.8664341873828154 >10 1.2593974084032977 11.277000829453003 >50 0.20122743553986588 6.3933478538074 >100 0.1841100539181729 15.987579831394024 >500 0.011405047538359081 3.4722084312378865 >1k 0.0036659081373297047 2.48151426764823 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3384 0.3079073220797938 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2259 0.20554451553730918 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1989 0.18097744196711288 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATGACGAGACGTAATTCGTGAAGGAACC 1803 0.16405345795208875 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACTGCAAATTCTAGAATTGTGAGTAGAA 1701 0.15477256349223678 No Hit GAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAA 1696 0.15431761768538133 No Hit GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTCTGAGCGTTCTATCATTCTAGCCTCG 1367 0.12438218359429026 No Hit TTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTC 1305 0.1187408555892822 No Hit CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGTACTGAAGTATTAAGTAGTGGGACTT 1302 0.11846788810516891 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGTGAGCTTTTTAGTTTGTGTCGGAAGC 1261 0.11473733248895393 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTAGAAGTCCCACTACTTAATACTTCAG 1231 0.112007657647821 No Hit ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTCATCATAGCCTTATAGAAGGTAAACG 1219 0.11091578771136783 No Hit CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCACTATGAGCTGGAGCCGTAATTACAG 1189 0.10818611287023491 No Hit TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATGGCATTAGCAGTCCGGCTTACAGCTA 1145 0.1041825897699066 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 14 0.0 0.0 0.0 0.0 0.0 15 0.0 0.0 0.0 0.0 0.0 16 0.0 0.0 0.0 9.098916137109748E-5 0.0 17 0.0 0.0 0.0 9.098916137109748E-5 0.0 18 0.0 0.0 0.0 9.098916137109748E-5 0.0 19 0.0 0.0 0.0 1.8197832274219496E-4 0.0 20 0.0 0.0 0.0 2.729674841132924E-4 0.0 21 0.0 0.0 0.0 4.549458068554874E-4 0.0 22 0.0 0.0 0.0 9.098916137109747E-4 0.0 23 0.0 0.0 0.0 0.0014558265819375597 0.0 24 0.0 0.0 0.0 0.001546815743308657 0.0 25 0.0 0.0 0.0 0.001728794066050852 0.0 26 0.0 0.0 0.0 0.002820664002504022 0.0 27 0.0 0.0 0.0 0.003275609809359509 0.0 28 0.0 0.0 0.0 0.0041855014230704835 0.0 29 0.0 0.0 0.0 0.0060962738118635305 0.0 30 0.0 0.0 0.0 0.00937188362122304 0.0 31 0.0 0.0 0.0 0.014831233303488888 0.0 32 0.0 0.0 0.0 0.02120047459946571 0.0 33 0.0 0.0 0.0 0.02702378092721595 0.0 34 0.0 0.0 0.0 0.034757859643759235 0.0 35 0.0 0.0 0.0 0.044766667394579955 0.0 36 0.0 0.0 0.0 0.055958334243224946 0.0 37 0.0 0.0 0.0 0.0716994591604248 0.0 38 0.0 0.0 0.0 0.09044322640287089 0.0 39 0.0 0.0 0.0 0.1115527118409655 0.0 40 0.0 0.0 0.0 0.13448198050648208 0.0 41 0.0 0.0 0.0 0.15631937923554545 0.0 42 0.0 0.0 0.0 0.17751985383501118 0.0 43 0.0 0.0 0.0 0.20044912250052774 0.0 44 0.0 0.0 0.0 0.22747290342774368 0.0 45 0.0 0.0 0.0 0.25467866267770184 0.0 46 0.0 0.0 0.0 0.2895275114828322 0.0 47 0.0 0.0 0.0 0.3210097613172319 0.0 48 0.0 0.0 0.0 0.35221904366751833 0.0 49 0.0 0.0 0.0 0.3878867949249885 0.0 50 0.0 0.0 0.0 0.4226446545687478 0.0 51 0.0 0.0 0.0 0.461133069828722 0.0 52 0.0 0.0 0.0 0.49543598366562575 0.0 53 0.0 0.0 0.0 0.5317406590526936 0.0 54 0.0 0.0 0.0 0.5695011610216991 0.0 55 0.0 0.0 0.0 0.6106282619614352 0.0 56 0.0 0.0 0.0 0.6578516367130347 0.0 57 0.0 0.0 0.0 0.7081686429512516 0.0 58 0.0 0.0 0.0 0.7614882915147148 0.0 59 0.0 0.0 0.0 0.817719593242053 0.0 60 0.0 0.0 0.0 0.874587819098989 0.0 61 0.0 0.0 0.0 0.936187481347222 0.0 62 0.0 0.0 0.0 0.9920548264290757 0.0 63 0.0 0.0 0.0 1.0542914128069065 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACATCGC 20 0.0073738354 51.723553 28 CCGAACG 20 0.007379144 51.714138 67 GGTATCA 2570 0.0 41.451294 1 GTATCAA 6385 0.0 40.379486 1 ATGGGTA 325 0.0 32.014893 5 GTGCGAA 45 0.0046228413 30.666342 23 AACGCGT 45 0.0046341782 30.650993 28 GTCGGAA 415 0.0 29.076221 67 TATCAAC 9025 0.0 28.399546 2 TGTGTCG 430 0.0 28.061935 64 ATCAACG 9200 0.0 27.897034 3 GAACGCG 50 0.007735171 27.599709 27 TCAACGC 9335 0.0 27.419289 4 CAACGCA 9510 0.0 26.914728 5 CATGGGT 560 0.0 26.631435 4 AACGCAG 9730 0.0 26.339409 6 GTGTCGG 475 0.0 25.403435 65 GACATAT 250 0.0 24.984196 1 GTTATCG 70 0.001339208 24.62578 49 TGCGGTA 85 1.4491528E-4 24.33828 51 >>END_MODULE