FastQCFastQC Report
Mon 6 Feb 2017
SRR2077310_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077310_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2313270
Sequences flagged as poor quality0
Sequence length46
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60390.26105902034781936No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT29730.1285193686858862No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28740.1242397126146105No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15450.025.2993621
GTATCAA38150.023.8541931
TACCGTT803.299847E-722.4929717
AATACCG605.4612523E-420.0041315
ATACCGT1701.8189894E-1218.817656
TATCAAC48600.018.5383782
ACCGTCG550.00779154518.176148
ATCAACG49950.017.7067153
TCAACGC50400.017.54714
CAACGCA51150.017.1687475
AACGCAG52900.016.7433516
CGCACGA1351.6350714E-716.30142435
AATCGGT956.924215E-414.7322419
ACGCAGA60750.014.448167
GTATAAT6300.014.3177691
CGCAGAG61300.014.28591258
CGATTCG850.005628874514.11323715
GTATTAT4750.013.9259161
TAACGCT1303.355166E-513.8520094
AGAGTAC61300.013.34004111
Produced by FastQC (version 0.11.5)