##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2077310_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2313270 Sequences flagged as poor quality 0 Sequence length 46 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.953828995318315 31.0 30.0 34.0 20.0 34.0 2 29.06742360381624 31.0 30.0 34.0 18.0 34.0 3 29.205410955054965 31.0 30.0 34.0 20.0 34.0 4 32.52935109174459 37.0 35.0 37.0 22.0 37.0 5 32.57090266160024 37.0 35.0 37.0 22.0 37.0 6 32.615217851785566 37.0 35.0 37.0 22.0 37.0 7 32.47757633134049 37.0 35.0 37.0 21.0 37.0 8 32.409107886238964 37.0 35.0 37.0 19.0 37.0 9 33.75721294963407 39.0 35.0 39.0 17.0 39.0 10 33.67749246737303 39.0 35.0 39.0 17.0 39.0 11 33.699568576084936 39.0 35.0 39.0 17.0 39.0 12 33.607709000678696 39.0 35.0 39.0 17.0 39.0 13 33.565850938282175 39.0 35.0 39.0 17.0 39.0 14 34.47875820807774 40.0 34.0 41.0 16.0 41.0 15 34.60367574904789 40.0 35.0 41.0 17.0 41.0 16 34.565282046626635 40.0 35.0 41.0 17.0 41.0 17 34.58172716544113 40.0 35.0 41.0 17.0 41.0 18 34.534257566129334 40.0 35.0 41.0 17.0 41.0 19 34.63756327622802 40.0 35.0 41.0 17.0 41.0 20 34.620796102486956 40.0 35.0 41.0 17.0 41.0 21 34.63239137670916 40.0 35.0 41.0 17.0 41.0 22 34.56401803507589 40.0 35.0 41.0 17.0 41.0 23 34.509701418338544 40.0 34.0 41.0 16.0 41.0 24 34.53105344382627 40.0 35.0 41.0 16.0 41.0 25 34.46016245401531 40.0 34.0 41.0 16.0 41.0 26 34.48283339169228 40.0 34.0 41.0 16.0 41.0 27 34.44537343241385 40.0 34.0 41.0 16.0 41.0 28 34.36996286641853 40.0 34.0 41.0 15.0 41.0 29 34.319638433905254 40.0 34.0 41.0 11.0 41.0 30 34.22553787495623 40.0 34.0 41.0 10.0 41.0 31 34.183680244848205 40.0 34.0 41.0 10.0 41.0 32 34.146298097498345 40.0 34.0 41.0 9.0 41.0 33 34.08073203733243 39.0 34.0 41.0 9.0 41.0 34 34.018085221353324 39.0 34.0 41.0 9.0 41.0 35 33.991515473766576 39.0 34.0 41.0 8.0 41.0 36 33.93295724234525 39.0 34.0 41.0 8.0 41.0 37 33.8438487509024 39.0 34.0 41.0 2.0 41.0 38 33.80051485559403 39.0 34.0 41.0 2.0 41.0 39 33.71520315397684 39.0 34.0 41.0 2.0 41.0 40 33.682141730105 39.0 33.0 41.0 2.0 41.0 41 33.529658448862435 39.0 33.0 41.0 2.0 41.0 42 33.473290190941825 39.0 33.0 41.0 2.0 41.0 43 33.36126824797797 39.0 33.0 41.0 2.0 41.0 44 33.257981990861424 39.0 33.0 41.0 2.0 41.0 45 33.134002083630534 39.0 33.0 41.0 2.0 41.0 46 33.00316608091576 39.0 33.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2704420486058048 1101 2 0.3240630094875172 1101 3 0.45188072051125516 1101 4 0.5656631036170907 1101 5 0.46941143142176855 1101 6 0.33632955027542977 1101 7 0.4846971830032061 1101 8 0.6001419237694137 1101 9 0.5678253660570007 1101 10 0.5477268652119491 1101 11 0.5854701013187054 1101 12 0.5263613642901532 1101 13 0.6055253998132883 1101 14 0.7559713961548127 1101 15 0.6362955337776626 1101 16 0.6505917250715925 1101 17 0.5676202070260175 1101 18 0.5015663231479834 1101 19 0.6252349236774819 1101 20 0.5961137441969484 1101 21 0.6005583985687437 1101 22 0.6430883367444267 1101 23 0.6563494425955838 1101 24 0.5853241141171708 1101 25 0.6256906642953766 1101 26 0.6355825704026898 1101 27 0.6500876939317521 1101 28 0.6456997970496658 1101 29 0.6353453254890411 1101 30 0.720061007000794 1101 31 0.6966179229598737 1101 32 0.7039755404198971 1101 33 0.5946440046119861 1101 34 0.6335266405588058 1101 35 0.7039826187122387 1101 36 0.567378702288579 1101 37 0.6089917025126894 1101 38 0.6601443336781969 1101 39 0.6948822565730381 1101 40 0.6207414114054757 1101 41 0.6615562345854045 1101 42 0.5500538673404876 1101 43 0.5942138725651418 1101 44 0.6414689842483341 1101 45 0.6024767565239983 1101 46 0.5922203373586044 1102 1 0.609632163759958 1102 2 0.6081993044732847 1102 3 0.7466541313630408 1102 4 0.8221913552642945 1102 5 0.7421523898599602 1102 6 0.7018938793540457 1102 7 0.70686934270649 1102 8 0.6102317565336577 1102 9 0.7638267000706591 1102 10 0.7071737380290202 1102 11 0.7240067096467016 1102 12 0.7646678064126533 1102 13 0.877263894056469 1102 14 1.136541158541398 1102 15 0.9856901429236515 1102 16 0.9957523951159288 1102 17 0.8953624066653347 1102 18 0.8961437618861581 1102 19 0.8608435851741802 1102 20 0.8804498747846239 1102 21 0.8524315464812844 1102 22 0.8334902771925314 1102 23 0.889246290566426 1102 24 0.8765913427215537 1102 25 0.9512518137675272 1102 26 0.8049713976385959 1102 27 0.8726265741684998 1102 28 0.8987708927837943 1102 29 0.8990142804953862 1102 30 0.9470762047203607 1102 31 0.8263786398101445 1102 32 0.8389978842605856 1102 33 0.9312594378711125 1102 34 0.9274320543717138 1102 35 0.9632063417738479 1102 36 0.8590162040593441 1102 37 0.9796225302184354 1102 38 0.9691245459568947 1102 39 0.9939834848514622 1102 40 0.9291897057198 1102 41 0.8514705761648429 1102 42 0.9268349243464797 1102 43 0.8733107577498203 1102 44 0.9055522650693106 1102 45 0.9894020903937815 1102 46 0.8741680328322374 1103 1 0.3400286622019095 1103 2 0.38666931985221353 1103 3 0.39837519037335056 1103 4 0.42891845691531927 1103 5 0.359711128702088 1103 6 0.4114217814298158 1103 7 0.4371586260896123 1103 8 0.46466647156889707 1103 9 0.5223305267905403 1103 10 0.49873814297257724 1103 11 0.44001220248858885 1103 12 0.3790207292238179 1103 13 0.3639266838266053 1103 14 0.5913316560278474 1103 15 0.5555844117212416 1103 16 0.5407416350126226 1103 17 0.5161919767501004 1103 18 0.5764210800687124 1103 19 0.40581592797760635 1103 20 0.44329067929211874 1103 21 0.42434771974425445 1103 22 0.39073771496705234 1103 23 0.4183714757268362 1103 24 0.4209493003518716 1103 25 0.4314644598266 1103 26 0.41227016998328025 1103 27 0.38122101905609895 1103 28 0.40471755457861036 1103 29 0.4383405925289452 1103 30 0.469981715660154 1103 31 0.39082706979571213 1103 32 0.4834078197514273 1103 33 0.5165045872474252 1103 34 0.5141264082551729 1103 35 0.41369768694544007 1103 36 0.476710720051976 1103 37 0.4637582181659994 1103 38 0.4851708662931884 1103 39 0.5375450490248213 1103 40 0.49117536291809216 1103 41 0.5618948819044505 1103 42 0.5895193664121834 1103 43 0.5942375933359543 1103 44 0.5391703615988916 1103 45 0.4569269063912955 1103 46 0.5033058264835617 1104 1 0.5648689212564477 1104 2 0.4166466266475055 1104 3 0.5436727485369097 1104 4 0.47937865538095537 1104 5 0.5829750220193688 1104 6 0.6705882971768915 1104 7 0.6075287319373857 1104 8 0.696094306922852 1104 9 0.7911236717252095 1104 10 0.7289367701569844 1104 11 0.7349551633863882 1104 12 0.7141521738091186 1104 13 0.6885837987712193 1104 14 0.9616085743433658 1104 15 0.9614315042447217 1104 16 0.897117286198764 1104 17 0.8282028803715775 1104 18 0.6578265113033765 1104 19 0.7722899277208342 1104 20 0.8403467695139781 1104 21 0.7907031094441379 1104 22 0.8821745799888205 1104 23 0.7438177088164011 1104 24 0.8753890145654353 1104 25 0.6965698952274906 1104 26 0.6373254575679965 1104 27 0.7947621197743047 1104 28 0.6917876994529166 1104 29 0.8198267976282452 1104 30 0.8817692582896228 1104 31 0.9785388142306033 1104 32 0.9152052593049476 1104 33 0.9710828924076793 1104 34 0.8922402619932583 1104 35 0.845550732927876 1104 36 0.8438428142291983 1104 37 0.7667989715565895 1104 38 0.7704411994131135 1104 39 0.7993316988905192 1104 40 0.8431023297404394 1104 41 0.8669546418105156 1104 42 0.8783180719897459 1104 43 0.8340287546345593 1104 44 0.7650107711478924 1104 45 0.6637441355416485 1104 46 0.6566664868906784 1105 1 -0.01548196719080508 1105 2 -0.11722063739581756 1105 3 0.1347272075279662 1105 4 0.11710505085066103 1105 5 0.20324781708628592 1105 6 0.2890185712760456 1105 7 0.07616776571327932 1105 8 0.23775056690662666 1105 9 0.31347207776182984 1105 10 0.38567880245026487 1105 11 0.3303963399190408 1105 12 0.35681055620755586 1105 13 0.2738319828595621 1105 14 0.5931523239819967 1105 15 0.38410340020799794 1105 16 0.49146674225878684 1105 17 0.1965279622147449 1105 18 0.21856238863632882 1105 19 0.2816479236616942 1105 20 0.27593572586752657 1105 21 0.2503601383759104 1105 22 0.21801308571436095 1105 23 0.3037850601787966 1105 24 0.2384658363539316 1105 25 0.20548171562959539 1105 26 0.25724467160353726 1105 27 0.25901958483718346 1105 28 0.4910228029844603 1105 29 0.2618404791630695 1105 30 0.3446377213347489 1105 31 0.3504709459173796 1105 32 0.23231789752672682 1105 33 0.40969893793008083 1105 34 0.18669678836560166 1105 35 0.4559487500380186 1105 36 0.5358356683303853 1105 37 0.5602733799793995 1105 38 0.48445711266970193 1105 39 0.5068475958987548 1105 40 0.3619152630913831 1105 41 0.4247983124019683 1105 42 0.49132679841011395 1105 43 0.4086811559511432 1105 44 0.47922706668389736 1105 45 0.4339751280290045 1105 46 0.507772493252439 1106 1 -0.2652394364995345 1106 2 -0.16753419159801197 1106 3 -0.11724915237111233 1106 4 0.03846993804227594 1106 5 0.031469121185907056 1106 6 0.02103841100802839 1106 7 0.0771893228086924 1106 8 0.048806978823975555 1106 9 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0.22315471776290963 1106 45 0.19972697656695004 1106 46 0.006788960216404405 1107 1 0.3002962472498112 1107 2 0.3049450275245249 1107 3 0.30013936600981594 1107 4 0.41490111102919514 1107 5 0.41092774714190483 1107 6 0.3618516808931034 1107 7 0.34465430145289133 1107 8 0.34115839726996455 1107 9 0.49635262632523336 1107 10 0.33772062548171533 1107 11 0.39180409130450045 1107 12 0.4434750754590624 1107 13 0.4119025368698317 1107 14 0.5611879025390465 1107 15 0.5277114623485843 1107 16 0.5733628086707014 1107 17 0.5089115963004218 1107 18 0.46810002535047346 1107 19 0.5413146369971145 1107 20 0.5349848125796939 1107 21 0.37242298518916783 1107 22 0.316102473665147 1107 23 0.40423676797760066 1107 24 0.5215767425141706 1107 25 0.37929612543799607 1107 26 0.2776472368834888 1107 27 0.4753555887325547 1107 28 0.44563295738622344 1107 29 0.5592992515661095 1107 30 0.5173027092318776 1107 31 0.638383674704393 1107 32 0.639134684177769 1107 33 0.53114326064415 1107 34 0.467217660555562 1107 35 0.4445081347154627 1107 36 0.4912966299220116 1107 37 0.6339304540968769 1107 38 0.5037658179578557 1107 39 0.5430413319083343 1107 40 0.659482630083005 1107 41 0.6051466733987709 1107 42 0.5782664348311854 1107 43 0.5634375010244383 1107 44 0.5128319925540197 1107 45 0.5420596239200322 1107 46 0.7188403949765387 1108 1 -0.11209957461792897 1108 2 -0.20410796867572145 1108 3 -0.1846155125701543 1108 4 -0.20716804848058956 1108 5 -0.18203527226705063 1108 6 -0.2703984538716995 1108 7 -0.24760079115146283 1108 8 -0.07978758109428696 1108 9 -0.21361352489545737 1108 10 -0.05078772335074433 1108 11 -0.17948907694312766 1108 12 -0.3741262897813087 1108 13 -0.2139764918451874 1108 14 -0.19466319481805527 1108 15 -0.12565982150607624 1108 16 -0.04400604612480663 1108 17 -0.01354873392744338 1108 18 0.05106152071766701 1108 19 -0.09803734421013388 1108 20 -0.0648742605959498 1108 21 -0.12789193640264074 1108 22 -0.0015506337337001241 1108 23 -0.13508303901473795 1108 24 -0.037586652636925066 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-0.6720608049757999 2215 17 -0.629842071569712 2215 18 -0.5628393888720709 2215 19 -0.6149957898956444 2215 20 -0.5333077178112333 2215 21 -0.43168317007160084 2215 22 -0.44435264647873396 2215 23 -0.449758337021521 2215 24 -0.5117772120245192 2215 25 -0.8930652054072752 2215 26 -0.6006377887408902 2215 27 -0.6314588745076151 2215 28 -0.767590306037846 2215 29 -0.5952017490694317 2215 30 -0.7332618385214218 2215 31 -0.7013143429554702 2215 32 -0.6973552148121342 2215 33 -0.4714335448945022 2215 34 -0.4715398949188625 2215 35 -0.5995874395005956 2215 36 -0.6822401096287862 2215 37 -0.6078590946705802 2215 38 -0.5110552508290738 2215 39 -0.7354877676549023 2215 40 -0.5490307162315489 2215 41 -0.46783211042338024 2215 42 -0.6364848261048124 2215 43 -0.5767292264916861 2215 44 -0.5106051905217157 2215 45 -0.6112032592625312 2215 46 -0.7361952357619046 2216 1 -1.9342011294989163 2216 2 -1.843766018513687 2216 3 -1.7479310754042388 2216 4 -1.6746913785889923 2216 5 -1.7413928113081845 2216 6 -2.0100697955002325 2216 7 -1.9008983292834962 2216 8 -1.983470570381364 2216 9 -2.27222127338462 2216 10 -2.285326430358964 2216 11 -2.234037551370861 2216 12 -2.579926939204835 2216 13 -2.4234762228856965 2216 14 -4.383033502810605 2216 15 -3.1959238455838204 2216 16 -2.8640250768667563 2216 17 -2.6352941032894996 2216 18 -2.8908466763101863 2216 19 -2.7004350798232792 2216 20 -2.716396472212029 2216 21 -2.858795435633464 2216 22 -2.749072102560575 2216 23 -2.8350636291228497 2216 24 -2.6632493302584415 2216 25 -2.6538069000191804 2216 26 -2.574975192325933 2216 27 -2.8545702870041687 2216 28 -2.8287300074804698 2216 29 -2.966771876920852 2216 30 -2.693098873631797 2216 31 -2.668821418288413 2216 32 -2.6771643898314075 2216 33 -2.4544571969429434 2216 34 -2.647194905090622 2216 35 -2.5482990167270003 2216 36 -2.940552271150743 2216 37 -2.731371455602357 2216 38 -2.963617040005829 2216 39 -2.877856587576069 2216 40 -2.7376465906776772 2216 41 -2.926469280537919 2216 42 -2.7651800232387593 2216 43 -2.792648415971268 2216 44 -2.836919700682639 2216 45 -2.85324699318047 2216 46 -2.99352137919454 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 136967.0 3 4128.0 4 11904.0 5 9010.0 6 7143.0 7 5821.0 8 5034.0 9 4502.0 10 4121.0 11 3852.0 12 3882.0 13 3963.0 14 4255.0 15 4350.0 16 4623.0 17 5121.0 18 5612.0 19 6799.0 20 9372.0 21 12830.0 22 15885.0 23 17885.0 24 19014.0 25 19612.0 26 20997.0 27 24082.0 28 26439.0 29 29360.0 30 33468.0 31 39695.0 32 48483.0 33 59586.0 34 75581.0 35 99855.0 36 134745.0 37 194695.0 38 347063.0 39 853536.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.253737646760705 18.126877486620707 13.610903554310937 24.00848131230765 2 15.306095966905458 20.294283284643686 39.08829969177161 25.311321056679247 3 18.109823386428005 25.52177939893332 29.561003211116528 26.807394003522152 4 12.482735740143097 16.958401838868063 38.40838771602223 32.15047470496661 5 13.850402813553634 37.862700866174556 34.47282197837937 13.814074341892438 6 31.378438314593627 37.403545630211774 17.85096421948151 13.367051835713081 7 27.849278294362527 32.396391255668384 21.989607784651167 17.764722665317926 8 26.48912578298253 34.28765340837862 20.367704591336075 18.855516217302778 9 26.643712147738913 15.91228001919361 19.296493708040998 38.147514125026476 10 14.49666489428385 29.060982937573222 33.78256753426967 22.65978463387326 11 33.926000855931214 23.688674473796834 24.05741655751382 18.32790811275813 12 23.324514648095555 25.196194132116005 30.2294155027299 21.249875717058536 13 27.61726906068034 22.57600712411435 26.014256874467744 23.79246694073757 14 22.315164248012554 21.566527037483734 27.13340855153095 28.98490016297276 15 23.399999567710577 29.189824771481003 23.84521444797721 23.564961212831214 16 23.24514648095553 28.672701414015656 25.256498376756713 22.8256537282721 17 22.19894097461633 28.259611223818865 26.62533680837295 22.916110993191857 18 22.27353526550501 27.96128567056248 27.43989846328891 22.325280600643595 19 23.248206641887762 27.51854743396543 27.481845950603002 21.751399973543805 20 23.553208468534365 26.99148633378033 27.36814711255556 22.087158085129747 21 23.64402713112947 26.762377821295946 27.267001266638136 22.32659378093645 22 23.331733865912753 26.522628141116257 27.26114980093115 22.88448819203984 23 23.250121797756503 26.664652194456224 27.050730548779406 23.034495459007868 24 22.83304449094934 26.79218089472981 27.253132704234766 23.121641910086083 25 23.14660190355478 26.702765934176618 27.187451772418992 22.96318038984961 26 22.955002997955685 26.915762848952745 27.258398706923572 22.870835446167998 27 23.044576182507665 27.025688922327873 27.03273705284368 22.896997842320783 28 23.082386578554097 26.842113681469343 27.11925587041279 22.95624386956377 29 23.18541231580481 27.034517401790303 27.03131842407983 22.748751858325058 30 23.174279159463556 26.992132669501657 27.05105443151739 22.782533739517394 31 23.26835354171134 26.62888979574898 27.070133907882997 23.032622754656686 32 23.07693305291917 26.80347304327903 27.0587853858973 23.0608085179045 33 23.20305063145581 26.666352531054034 26.93290668529577 23.19769015219438 34 23.38128267922644 26.619776457339494 27.11713834268904 22.881802520745026 35 23.37978533744421 26.72189146520077 26.96233635308826 22.935986844266758 36 23.289480341396903 26.822325112356804 26.90392514764331 22.984269398602983 37 23.366204368201704 26.578112300482477 27.008534075777753 23.04714925553807 38 23.199155381889653 26.717273426090294 27.049439731465398 23.034131460554658 39 23.368115836722055 26.846928439389263 26.873052080332794 22.911903643555895 40 23.433785129341 26.58928098154841 27.201062554341554 22.775871334769036 41 23.307382174109943 26.72395559163928 26.992997555697524 22.975664678553244 42 23.267865067837214 26.70929615919317 26.980074035689043 23.042764737280578 43 23.22053231610344 26.604779031827064 27.01036803093892 23.16432062113058 44 23.310058184988133 26.75143040978559 26.795363189173855 23.143148216052413 45 23.416941278415884 26.782850389962377 26.62822318378974 23.171985147831997 46 23.427369873888644 26.60955637479364 26.690629813059786 23.27244393825793 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1073.0 1 1136.5 2 1200.0 3 1237.5 4 1275.0 5 1251.0 6 1227.0 7 1227.0 8 1173.5 9 1120.0 10 1358.5 11 1597.0 12 2165.5 13 2734.0 14 5069.5 15 7405.0 16 10117.5 17 12830.0 18 13360.0 19 13890.0 20 13890.0 21 15560.5 22 17231.0 23 21545.5 24 25860.0 25 32179.5 26 38499.0 27 44383.5 28 50268.0 29 56947.0 30 63626.0 31 63626.0 32 70710.5 33 77795.0 34 86979.5 35 96164.0 36 104510.0 37 112856.0 38 120145.5 39 127435.0 40 134946.0 41 142457.0 42 148332.0 43 154207.0 44 154207.0 45 159005.0 46 163803.0 47 168093.5 48 172384.0 49 174435.5 50 176487.0 51 173568.0 52 170649.0 53 164390.0 54 158131.0 55 148496.0 56 138861.0 57 138861.0 58 127590.5 59 116320.0 60 103234.5 61 90149.0 62 77748.5 63 65348.0 64 54857.0 65 44366.0 66 36350.0 67 28334.0 68 22563.0 69 16792.0 70 16792.0 71 13068.0 72 9344.0 73 7377.5 74 5411.0 75 4208.0 76 3005.0 77 2269.0 78 1533.0 79 1138.5 80 744.0 81 744.0 82 594.0 83 444.0 84 324.5 85 205.0 86 155.0 87 105.0 88 74.5 89 44.0 90 29.0 91 14.0 92 16.0 93 18.0 94 18.0 95 14.0 96 10.0 97 7.0 98 4.0 99 10.0 100 16.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2016625815404168 2 0.058272488728077575 3 0.06972813376735099 4 0.06108236392638991 5 0.044698630077768704 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 2.161442460240266E-4 16 0.0 17 4.7551734125285855E-4 18 7.781192856864958E-4 19 5.187461904576639E-4 20 0.0020749847618306556 21 0.002593730952288319 22 0.0 23 3.890596428432479E-4 24 5.619750396624691E-4 25 8.21348134891301E-4 26 0.0011671789285297436 27 0.02593730952288319 28 7.348904364816904E-4 29 0.001340094325348965 30 0.0014265520237585756 31 0.001642696269782602 32 0.001080721230120133 33 0.0019020693650114342 34 0.0028098751983123456 35 0.0025072732538787084 36 0.0333294427369049 37 0.00821348134891301 38 0.011758246983707046 39 0.051355872855308723 40 0.015259783769296277 41 0.058920921466149655 42 0.06103913507718511 43 0.025245647935606307 44 0.027925836586304233 45 0.026412826864136048 46 0.023862324761052534 >>END_MODULE >>Sequence Length Distribution pass #Length Count 46 2313270.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.55618917020381 #Duplication Level Percentage of deduplicated Percentage of total 1 82.9092767887012 57.668533402796896 2 10.353776915954477 14.40338531584438 3 3.0675210092737335 6.400952148138549 4 1.2570374298632763 3.497389330623874 5 0.662265345323957 2.303232682011175 6 0.41771156607029786 1.743265482490064 7 0.28021838425147355 1.3643646060775048 8 0.1941171497774291 1.0801639352879717 9 0.13159544211668858 0.8237949719224495 >10 0.6936441347090918 8.23309293104837 >50 0.025641463646592054 1.175647852740643 >100 0.006944763074921655 0.75502927973272 >500 6.240180921992558E-5 0.035244308218912584 >1k 1.2480361843985116E-4 0.2537850617684506 >5k 6.240180921992558E-5 0.2621186912980457 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6039 0.26105902034781936 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2973 0.1285193686858862 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2874 0.1242397126146105 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 8.645769840961064E-5 0.0 3 0.0 0.0 0.0 8.645769840961064E-5 0.0 4 0.0 0.0 0.0 8.645769840961064E-5 0.0 5 0.0 0.0 0.0 8.645769840961064E-5 0.0 6 0.0 0.0 0.0 8.645769840961064E-5 0.0 7 0.0 0.0 0.0 8.645769840961064E-5 0.0 8 0.0 0.0 0.0 8.645769840961064E-5 0.0 9 0.0 0.0 0.0 8.645769840961064E-5 0.0 10 0.0 0.0 0.0 1.2968654761441595E-4 0.0 11 0.0 0.0 0.0 1.2968654761441595E-4 0.0 12 0.0 0.0 0.0 1.2968654761441595E-4 4.322884920480532E-5 13 0.0 0.0 0.0 1.2968654761441595E-4 4.322884920480532E-5 14 0.0 0.0 0.0 1.2968654761441595E-4 4.322884920480532E-5 15 0.0 0.0 0.0 1.2968654761441595E-4 4.322884920480532E-5 16 0.0 0.0 0.0 1.7291539681922127E-4 4.322884920480532E-5 17 0.0 0.0 0.0 1.7291539681922127E-4 4.322884920480532E-5 18 0.0 0.0 0.0 1.7291539681922127E-4 4.322884920480532E-5 19 0.0 0.0 0.0 1.7291539681922127E-4 4.322884920480532E-5 20 0.0 0.0 0.0 2.161442460240266E-4 4.322884920480532E-5 21 0.0 4.322884920480532E-5 0.0 2.161442460240266E-4 4.322884920480532E-5 22 0.0 4.322884920480532E-5 0.0 2.161442460240266E-4 4.322884920480532E-5 23 0.0 4.322884920480532E-5 0.0 2.161442460240266E-4 4.322884920480532E-5 24 0.0 4.322884920480532E-5 0.0 3.4583079363844255E-4 4.322884920480532E-5 25 0.0 4.322884920480532E-5 0.0 3.4583079363844255E-4 4.322884920480532E-5 26 0.0 4.322884920480532E-5 0.0 5.187461904576638E-4 4.322884920480532E-5 27 0.0 4.322884920480532E-5 0.0 5.619750396624691E-4 4.322884920480532E-5 28 0.0 4.322884920480532E-5 0.0 8.645769840961064E-4 4.322884920480532E-5 29 0.0 4.322884920480532E-5 0.0 0.0015562385713729914 4.322884920480532E-5 30 0.0 4.322884920480532E-5 0.0 0.0023775867062642924 4.322884920480532E-5 31 0.0 4.322884920480532E-5 0.0 0.004193198372866116 1.7291539681922127E-4 32 0.0 4.322884920480532E-5 0.0 0.006268183134696771 1.7291539681922127E-4 33 0.0 4.322884920480532E-5 0.0 0.008991600634599506 1.7291539681922127E-4 34 0.0 4.322884920480532E-5 0.0 0.012882197063031986 2.593730952288319E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1545 0.0 25.299362 1 GTATCAA 3815 0.0 23.854193 1 TACCGTT 80 3.299847E-7 22.492971 7 AATACCG 60 5.4612523E-4 20.004131 5 ATACCGT 170 1.8189894E-12 18.81765 6 TATCAAC 4860 0.0 18.538378 2 ACCGTCG 55 0.007791545 18.17614 8 ATCAACG 4995 0.0 17.706715 3 TCAACGC 5040 0.0 17.5471 4 CAACGCA 5115 0.0 17.168747 5 AACGCAG 5290 0.0 16.743351 6 CGCACGA 135 1.6350714E-7 16.301424 35 AATCGGT 95 6.924215E-4 14.73224 19 ACGCAGA 6075 0.0 14.44816 7 GTATAAT 630 0.0 14.317769 1 CGCAGAG 6130 0.0 14.2859125 8 CGATTCG 85 0.0056288745 14.113237 15 GTATTAT 475 0.0 13.925916 1 TAACGCT 130 3.355166E-5 13.852009 4 AGAGTAC 6130 0.0 13.340041 11 >>END_MODULE